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Showing: 6751 - 6800 of 157662 results

  2. pSaCas9_GFP

    Plasmid
    #64709
    Purpose
    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells
    Depositor
    Kiran Musunuru
    Insert
    SaCas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP and 3xFLAG-SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64709-plasmid-map-sequence-id-322473

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  5. pcDNA3.1(+)-HLA-cmyc-optiT1R3 a21-852

    Plasmid
    #113949
    Purpose
    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R3 (TAS1R3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    residues 21-852
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113949-plasmid-map-sequence-id-224194

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  7. IDG_GABRP_OE_1

    Plasmid
    #161679
    Purpose
    Overexpresses GABRP
    Depositor
    Mike McManus
    Insert
    GABRP (GABRP Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    161679-plasmid-map-sequence-id-319658

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  11. pENN.AAV.CamKII.GCaMP6f.WPRE.SV40

    Plasmid
    #100834
    Purpose
    AAV expression of ultrasensitive protein calcium sensor from CamKII promoter
    Depositor
    James M. Wilson
    Has Service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G
    Promoter
    CamKII
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    100834-plasmid-map-sequence-id-259370

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  22. pFastBac1-pGC-A

    Plasmid
    #186626
    Purpose
    pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)
    Depositor
    Debra Hansen
    Article
    Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1
    Insert
    NPR1 (NPR1 Human)
    Use
    Tags
    hemagglutinin (HA) signal peptide + His10-tag + T…
    Expression
    Insect
    Mutation
    deleted amino acids 1-32
    Promoter
    polyhedring
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    186626-plasmid-map-sequence-id-366906

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  23. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid
    #208942
    Purpose
    The CE1 construct, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208942-plasmid-map-sequence-id-415667

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  24. pAAV-anti-PDL1(KN035) PAGER(TF)

    Plasmid
    #230003
    Purpose
    Expresses anti-PDL1 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-KN035-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230003-plasmid-map-sequence-id-457206

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  28. PX551

    Plasmid
    #60957
    Purpose
    pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.
    Depositor
    Feng Zhang
    Article
    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    pMecp2
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    60957-plasmid-map-sequence-id-181490

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  29. mEmerald-Homer1-N-18

    Plasmid
    #54121
    Purpose
    Localization: Membrane Adaptor, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Homer1 (Homer1 Rat)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. pRPA70-IRES-RPA32-IRES-RPA14

    Plasmid
    #211432
    Purpose
    mammalian expression of human RPA70 and RPA32 and RPA14
    Depositor
    Helmut Pospiech
    Insert
    RPA70 - IRES - RPA32 - IRES - RPA14 (RPA1, RPA2, RPA3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211432-plasmid-map-sequence-id-420528

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  32. R777-E209 Hs.RAC2

    Plasmid
    #70493
    Purpose
    Gateway ORF clone of human RAC2 [NM_002872.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RAC2 (RAC2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70493-plasmid-map-sequence-id-125942

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  37. pBG-ttLbUV2

    Plasmid
    #231386
    Purpose
    LbCas12a variant with D156R and E795L mutations and harboring same NLS sequence as PEmax but codon-optimized for dicot species; for Agrobacterium-mediated Arabidopsis transformation.
    Depositor
    Qi-Jun Chen
    Article
    Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:
    Insert
    ttLbCas12a Ultra V2
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D156R and E795L
    Promoter
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    231386-plasmid-map-sequence-id-461803

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  40. pAAV-CAG-JNKKTRrsGreenF-E-2A-ERKKTRrsFastLime-IRES-PKAKTRClover-2A-P38KTRDronpa

    Plasmid
    #205766
    Purpose
    Expression of JNK KTR rsGreenF-E, ERK KTR rsFastLime, PKA KTR Clover, and P38 KTR Dronpa in mamalian cells
    Depositor
    Edward Boyden
    Article
    Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.
    Insert
    JNK KTR rsGreenF-E-2A-ERK KTR rsFastLime-IRES-PKA KTR Clover-2A-P38 KTR Dronpa
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    205766-plasmid-map-sequence-id-459162

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  43. pCHW110031

    Plasmid
    #222437
    Purpose
    Level 0, promoter module (GGAG-CCAT) containing CBS4 and CMV1 promoters.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Insert
    CBS4-CMV1, , CBS4 promoter + CMV1 (Cucumber mosaic virus)
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    no
    Promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222437-plasmid-map-sequence-id-454095

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  44. pLV_CAG_Gag-MCP

    Plasmid
    #212637
    Purpose
    Lentivirus vector to express an MLV Gag-MCP fusion protein
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    MLV Gag (UniProt ID: P03332) (gag MoMLV)
    Use
    Lentiviral
    Tags
    MS2 Capsid Protein (N55K)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212637-plasmid-map-sequence-id-422309

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  50. LT3GEPIR-CDK4shRNA1

    Plasmid
    #220574
    Purpose
    To inducibly knockdown CDK4 expression
    Depositor
    Sean Lee
    Insert
    CDK4 shRNA1 (CDK4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TRE3G (TetOP) promoter
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220574-plasmid-map-sequence-id-437920

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Showing: 6751 - 6800 of 157662 results