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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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159,258 results

Showing: 6751 - 6800 of 159258 results

  1. FLAG-NM23-H1

    Plasmid
    #25000
    Depositor
    Judy Lieberman
    Insert
    NM23-H1 (NME1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2010
    Availability
    Academic Institutions and Nonprofits only

  4. HCN3/pCDNA3.1

    Plasmid
    #197293
    Purpose
    Plasmid expressing an antigen targeting the HCN3 ion channel subunit
    Depositor
    Dane Chetkovich
    Insert
    HCN3 (Hcn3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197293-plasmid-map-sequence-id-388075

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  15. pTER_MDC1 (422-2)

    Plasmid
    #22191
    Depositor
    Eric Campeau
    Insert
    sh Mediator of DNA damage checkpoint 1
    Use
    RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 8, 2010
    Availability
    Academic Institutions and Nonprofits only
    22191-plasmid-map-sequence-id-10167

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  26. pcDNA3.1-FLT3-D835Y-V5/HIS

    Plasmid
    #236007
    Purpose
    expression of the D835Y kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia and Acute Lymphoblastic Leukemia
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    human FLT3-D835Y receptor tyrosine kinase, full length (FLT3 Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    D835Y substitution
    Promoter
    CMV
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    236007-plasmid-map-sequence-id-469607

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  27. pcDNA3.1-FLT3-D835N-V5/HIS

    Plasmid
    #236008
    Purpose
    expression of the D835N kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    human FLT3-D835N receptor tyrosine kinase, full length (FLT3 Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    D835N substitution
    Promoter
    CMV
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    236008-plasmid-map-sequence-id-469587

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  28. pRSET-iGluSnFR4f

    Plasmid
    #234444
    Purpose
    Bacterial expression of glutamate sensor with with improved sensitivity and fast deactivation kinetics (4f = fast)
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f
    Use
    Tags
    6xHis-Xpress epi
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234444-plasmid-map-sequence-id-464950

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  32. pHEE-R-bar5A9si

    Plasmid
    #222837
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, bialaphos and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222837-plasmid-map-sequence-id-446488

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  36. pOET5 rota-VLP

    Plasmid
    #218147
    Purpose
    Forms rotavirus-like particles in insect cells by expression of VP6 (GenBank ID: ACL93331.1).
    Depositor
    Minna Hankaniemi
    Insert
    Rotavirus VP6
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218147-plasmid-map-sequence-id-433323

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  37. pOET5 CVB3-VLP

    Plasmid
    #218146
    Purpose
    Forms coxsackievirus 3-like particle in insect cells by expression of VP1–VP4 polyprotein (GeneID M33854.1) and 3CD protease (GeneID M33854.1).
    Depositor
    Minna Hankaniemi
    Inserts
    VP1–VP4
    3CD
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    CMV and Polyhedrin
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218146-plasmid-map-sequence-id-433321

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  38. pOET5 GII.4 Sydney noro-VLP

    Plasmid
    #218092
    Purpose
    Forms Sydney GII.4 Sydney norovirus-like particle in insect cells by expression of VP1 from strain GII.4/Sydney/NSW0514/2012/AU (GenBank ID: AFV08795).
    Depositor
    Minna Hankaniemi
    Insert
    noro-VLP
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218092-plasmid-map-sequence-id-433318

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  39. CRHR1-DuET

    Plasmid
    #213215
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CRHR1 (CRHR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213215-plasmid-map-sequence-id-430777

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  40. CRHR2-DuET

    Plasmid
    #213216
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CRHR2 (CRHR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213216-plasmid-map-sequence-id-430778

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  42. pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON)(TRE-SEAP

    Plasmid
    #210513
    Purpose
    expresses PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON and SEAP reporter in mammalian cells
    Depositor
    Dongmin Lee
    Article
    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d
    Inserts
    secreted alkaline phosphatase
    CMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and TetO
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    210513-plasmid-map-sequence-id-415575

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  43. qTAG-N-Blast-3xFLAG-LMNB1

    Plasmid
    #207777
    Purpose
    Donor template for Blast-2A-3xFLAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Blast-3xFLAG Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207777-plasmid-map-sequence-id-416194

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Showing: 6751 - 6800 of 159258 results