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171,804 results

Showing: 6751 - 6800 of 171804 results

  2. pOpen-MMLV_RT (mut H)

    Plasmid
    #165546
    Purpose
    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
    Use
    Synthetic Biology
    Available Since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165546-plasmid-map-sequence-id-339423

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  4. TFORF2386

    Plasmid
    #143589
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MBD2 (MBD2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    143589-plasmid-map-sequence-id-391946

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  7. pCW57-mCherry-2A-BRD4 Iso CdelET

    Plasmid
    #137723
    Purpose
    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long short missing its extra-terminal domain (ET)
    Depositor
    Scott Floyd
    Article
    Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi
    Insert
    BRD4 short isoform with deleted extra-terminal domain (BRD4 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Deleted amino acids 600-682
    Promoter
    Tight TRE promoter
    Available Since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    137723-plasmid-map-sequence-id-288120

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  13. MADR pDonor-H3F3A-K27M-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131462
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A K27M, PDGFRA D842V, and TRP53
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    H3F3A-K27M-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    H3F3A K27M, Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131462-plasmid-map-sequence-id-305183

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  14. pEcTrpRSH6

    Plasmid
    #177598
    Purpose
    Expresses His tagged TrpRS in Escherichia coli cells
    Depositor
    Kambiz Hamadani
    Insert
    Tryptophan tRNA Synthetase (trpS Escherichia coli)
    Tags
    Hexahistidine
    Expression
    Bacterial
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    177598-plasmid-map-sequence-id-351508

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  15. pEcH6ArgRS

    Plasmid
    #177582
    Purpose
    Expresses His tagged ArgRS in Escherichia coli cells
    Depositor
    Kambiz Hamadani
    Insert
    Arginine tRNA Synthetase (argS Escherichia coli)
    Tags
    Hexahistidine
    Expression
    Bacterial
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    177582-plasmid-map-sequence-id-350694

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  16. pEcCysRSH6

    Plasmid
    #177585
    Purpose
    Expresses His tagged CysRS in Escherichia coli cells
    Depositor
    Kambiz Hamadani
    Insert
    Cysteine tRNA Synthetase (cysS Escherichia coli)
    Tags
    Hexahistidine
    Expression
    Bacterial
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    177585-plasmid-map-sequence-id-356947

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  17. pA-CBh.TP-DCP2-V5H6.MCh.Puro

    Plasmid
    #209948
    Purpose
    In mammalian cells expresses TP fused to a DCP2 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.
    Depositor
    Kevin Weeks
    Article
    Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38
    Inserts
    Decapping protein 2 (DCP2 Human)
    mCherry fluorescent protein fused to puromycin resistance marker
    Tags
    TP (MS2 coat protein) and V5 epitope with H6 tag
    Expression
    Mammalian
    Promoter
    CBh and CMV
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    209948-plasmid-map-sequence-id-418740

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  18. pET30a-SSDH

    Plasmid
    #239573
    Purpose
    Expresses E. coli succinate semialdehyde dehydrogenase (SSDH/gabD) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.
    Depositor
    William Plaxton
    Article
    Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b
    Insert
    Succinate-semialdehyde dehydrogenase (gabD E. coli)
    Tags
    His6
    Expression
    Bacterial
    Available Since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    239573-plasmid-map-sequence-id-476594

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  20. Anti-GAD65/GAD67 [L127/12-rat IgG2a] - Chimeric

    Plasmid
    #227046
    Purpose
    Mammalian expression plasmid of anti-GAD65/GAD67 (Homo sapiens) rat IgG2a R-mAb. Derived from hybridoma L127/12.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-GAD65/GAD67 (Homo sapiens) recombinant mouse monoclonal antibody (GAD1, GAD2 Chimera: M.musculus (mouse)/R.norvegicus (rat))
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Promoter
    Dual CMV
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    227046-plasmid-map-sequence-id-459983

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  23. pLV-SFFV-ZFYVE27-WPRE-UbC-Emerald

    Plasmid
    #214469
    Purpose
    Lentiviral vector plasmid expressing human zinc finger FYVE-type containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    ZFYVE27 (ZFYVE27 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    214469-plasmid-map-sequence-id-425107

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  25. TFORF2387

    Plasmid
    #144019
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MBD2 (MBD2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    144019-plasmid-map-sequence-id-427328

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  26. TFORF3309

    Plasmid
    #144785
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    DCP1A (DCP1A Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144785-plasmid-map-sequence-id-404368

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  27. pT7-EGFP-C1-HsDCP1b_N

    Plasmid
    #147022
    Purpose
    Mammalian Expression of HsDCP1b
    Depositor
    Elisa Izaurralde
    Insert
    HsDCP1b (DCP1B Human)
    Expression
    Mammalian
    Mutation
    inserted Q555
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    147022-plasmid-map-sequence-id-355296

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  36. pcDNA3-HA-SOX4

    Plasmid
    #110367
    Purpose
    Mammalian expression of HA-tagged SOX4
    Depositor
    Carlos Moreno
    Insert
    SOX4 (SOX4 Human)
    Tags
    HA
    Expression
    Mammalian
    Available Since
    Jan. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    110367-plasmid-map-sequence-id-211161

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  37. pENN.AAV.CamKII 0.4.Cre.SV40

    Plasmid
    #105558
    Purpose
    AAV expression of Cre from CamKII promoter
    Depositor
    James M. Wilson
    Has Service
    AAV Retrograde, AAV1, AAV5, and AAV9
    Insert
    Cre
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    CamKII
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105558-plasmid-map-sequence-id-202399

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  42. pSPIKE-P

    Plasmid
    #101172
    Purpose
    chimeric spike resembling V4 region of prokaryotic 16S rRNA to be used in metagenomics
    Depositor
    Philip Poole
    Insert
    V4 16S rRNA chimeric spike
    Use
    Synthetic Biology
    Available Since
    Oct. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    101172-plasmid-map-sequence-id-194323

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  45. FLAG-hPAX3 (#395)

    Plasmid
    #78335
    Purpose
    Overexpression of human PAX3 ORF with a N-terminal FLAG tag
    Depositor
    Matthew Alexander, Louis Kunkel
    Insert
    PAX3 (PAX3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 6751 - 6800 of 171804 results