161,255 results

1. PTGS1-bio-His

   Plasmid

   #53414

   Purpose

   Expresses full-length Prostaglandin G/H synthase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PTGS1 (PTGS1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-IRES-puro-NLS-dEsCas13d-EGFP-NLS-3xFlag

   Plasmid

   #132410

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dEsCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   R295A; H300A; R849A; H854A

   Promoter

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. 560HTR

   Plasmid

   #24444

   Depositor

   Ron Vale

   Article

   Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

   Insert

   Kinesin (KIF5B Human)
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pRS428

   Plasmid

   #11257

   Depositor

   Fred Cross

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. 12x PRE TK luc

   Plasmid

   #206163

   Purpose

   Luciferase reporter construct containing 12 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

   Depositor

   Renee van Amerongen

   Article

   Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

   Insert

   12X PRE TK luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   min TK

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR223-GFP

   Plasmid

   #198589

   Purpose

   Gateway Entry Clone

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   GFP
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   None (wildtype)

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. JDW 762 (pCS2-EGFP-hsKRAS4B-G12V)

   Plasmid

   #156407

   Purpose

   pCS2 CMV expression vector driving EGFP fused human KRAS4B-G12V

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4B-G12V (KRAS Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   mutant KRAS4B-G12V

   Promoter

   CMV

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV2-CAG-3xNLS-AausFP1

   Plasmid

   #191096

   Purpose

   To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   3xNLS-AausFP1
   

   Use

   AAV and Synthetic Biology

   Tags

   Three repeats of the nuclear localizzation seque…

   Expression

   Mammalian

   Mutation

   Optimized to Human codon usage

   Promoter

   CMV enhancer and CAG

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRF2ΔBΔM/pENTR

   Plasmid

   #19797

   Depositor

   Mark Mattson

   Article

   van Steensel et al Cell. 1998 Feb 6. 92(3):401-13.

   Insert

   TRF2 (TERF2 Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   deleted amino acids 1-44 and 450-500

   Promoter

   Available since

   April 15, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-PTK7DN-VSV

    Plasmid

    #65251

    Purpose

    Expression of VSV tagged dominant negative PTK7 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gartner et al PLoS One. 2014 Jan 7;9(1):e84472. doi: 1

    Insert

    PTK7DN (PTK7 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    deleted AA 737-1071

    Promoter

    CMV

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

11. TFORF2215

    Plasmid

    #141974

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CREB3L4 (CREB3L4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCK007 pME KalTa4

    Plasmid

    #195965

    Purpose

    Gateway pME Gal4 variant

    Depositor

    Christian Mosimann, Alexa Burger

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    KalTA4 (Gal4)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. PRS_8-ChrimsonR-tdTomato

    Plasmid

    #192587

    Purpose

    AAV-vector for optogenetic activation of noradrenergic neurons

    Depositor

    Simon Wiegert

    Article

    Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

    Insert

    ChrimsonR
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    PRSX8

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX459-CDKN1A

    Plasmid

    #217457

    Purpose

    For CDKN1A (p21) knockout

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    CRISPR Cas9 guide for CDKN1A
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV Cag Flex H2B Gcamp6f reverse

    Plasmid

    #74155

    Purpose

    Calcium Sensor

    Depositor

    Loren Looger

    Insert

    Gcamp6f
    

    Use

    AAV

    Tags

    H2B

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCardinal-H1-10

    Plasmid

    #56161

    Purpose

    Localization: Nucleus/Histones, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    H1 (H1f0 Mouse)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pcDNA4/HisMax-Synectin

    Plasmid

    #35943

    Depositor

    Arie Horowitz

    Article

    Naccache et al Proc Natl Acad Sci U S A. 2006 Aug 22;10

    Insert

    Synectin (Gipc1 Mouse)
    

    Use

    Tags

    6xHIS and Xpress

    Expression

    Mammalian

    Mutation

    K111R

    Promoter

    CMV

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

18. LentiCRISPRv2puro-Ptgs2_B6

    Plasmid

    #170300

    Purpose

    A knock-out vector for the mouse Ptgs2 in C57BL6

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse tyrosinase gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. LV_EF1a_PTEN(C124S)-P2A-Hygro_Barcode

    Plasmid

    #170248

    Purpose

    Barcoded lentiviral vector to express PTEN (C124S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    C124S

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT3TS-NLS-TurboID

    Plasmid

    #163849

    Purpose

    In vitro transcription of nls-TurboID. Parton lab clone JST

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    TurboID
    

    Use

    In vitro transcription of rna

    Tags

    nls

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLKO_HA.V5.VSVg_NGFR

    Plasmid

    #158244

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HA.V5.VSVg

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. p7270 MSCV-N-HA-IRES-PURO Empty

    Plasmid

    #163302

    Purpose

    Empty vector control

    Depositor

    Peter Howley, Elizabeth White

    Article

    White et al J Virol. 2014 Aug;88(15):8201-12. doi: 1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. YFVdelNS1-mCherry

    Plasmid

    #179951

    Purpose

    pCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) but with mCherry

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    YFV-17D lacking nonstructural protein 1 (NS1) but with mCherry
    

    Use

    Transneuronal tracing

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. 1056 pSG5L HA PTEN 380-385E

    Plasmid

    #11171

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E4: S380E, T382E, T383E, S385E

    Promoter

    Available since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

25. pETM6-H10-mCherry

    Plasmid

    #66531

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter H10

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - H10

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pKDR-Lenti-CBh.6-SOD1

    Plasmid

    #188460

    Purpose

    For knockdown-replacement of SOD1 gene (SOD1 KDR lentiviral transfer plasmid, Fig 4)

    Depositor

    Erik Dent

    Article

    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CBh

    Available since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pMEH380 VSV-G HA

    Plasmid

    #168222

    Purpose

    Mammalian expression of vesicular stomatitis virus glycoprotein fused to a C-terminal HA tag

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    VSV-G
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pNIR-Fb59H10

    Plasmid

    #184682

    Purpose

    NIR-Fb to HIV p24

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-Fb59H10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBluescript II KS(+) EACMCV DNA-A 1.6mer

    Plasmid

    #159136

    Purpose

    Infectious clone containing a partial-tandem-dimer of East African cassava mosaic Cameroon virus 1998 DNA-A

    Depositor

    Vincent Fondong

    Article

    Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(

    Insert

    EACMCV DNA-A 1.6mer
    

    Use

    Infectious clone for bombarding plants

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pW267-lenti-spsgRNA-hsCDH1-sg1-pEF1s-NLS-mScarlet-I-P2A-BlastR

    Plasmid

    #170816

    Purpose

    Lentiviral vector to co-express a human CDH1 spsgRNA (sg1-Cdh1) with NLS-mScarlet-I

    Depositor

    Kenneth Yamada

    Article

    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

    Insert

    NLS-mScarlet-I-P2A-BlastR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge