169,365 results
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pCAG-BirA*-dCas9-eGFP
Plasmid#84779PurposeExpresses BirA*-dCas9-eGFP in mammalian cells.DepositorInsertBirA*-dCas9-eGFP
UseCRISPRTagscMycExpressionBacterial and MammalianPromoterCAGAvailable SinceJuly 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-EGFP
Plasmid#30130DepositorInsertEnhanced green fluorescent protein
UseLentiviralExpressionMammalianAvailable SinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-ECC-Biosensor
Plasmid#140832PurposeEncoding the CAG promoter based eccDNA biosensorDepositorInsertECC-Biosensor
UsePiggybac (pb) transposonExpressionMammalianAvailable SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-HyperdLbCas12a-StayGold-NLS-3xFlag
Plasmid#236368PurposeOverexpression of HyperdLbCas12a in human cellsDepositorInsertHyperdLbCas12a
UseCRISPR and LentiviralMutationD156R, D235R, E292R, D350RAvailable SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
Cas9-sgRNA-A+B
Plasmid#149370PurposeExpresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sitesDepositorInsertsCas9
sgRNA-A
sgRNA-B
Puromycin resistance
UseCRISPRTags3xFLAG, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianPromoterCBh, PGK, and U6Available SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hysn-flex-dsRed-shscramble
Plasmid#71383PurposeExpresses scramble shRNA Cre-dependently by flex switchDepositorInserthuman synapsin promoter-flex switch-dsRed-shscramble
UseAAV, Cre/Lox, and RNAiExpressionMammalianPromoterhuman synapsinAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9- AsiA_m2.1
Plasmid#158065PurposeExpressing evolved CasTA of dCas9 fused AsiA variant 2.1DepositorInsertdCas9-AsiA_m2.1
ExpressionBacterialPromoterTetOAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDonor_AcCAST_kanR
Plasmid#127925PurposeKanamycin resistance donor for AcCASTDepositorInsertKanR
Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINE GFP nanobody
Plasmid#49172PurposeBacterial expression of GFP nano bodyDepositorInsertGFP nanobody
TagsHHHHHHExpressionBacterial and MammalianPromoterT7Available SinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
GALT-SEP-mRuby3
Plasmid#200938Purposetrans-Golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3DepositorInsertsUseLentiviralAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-VSVgSS-15F11-TM
Plasmid#207317PurposeFunctional incorporation of 15F11 scFv (anti-HA) into envelope of virionsDepositorInsert15F11 ScFv
ExpressionMammalianPromoterCMV promoterAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Green cGull
Plasmid#86867PurposeGreen fluorescent probe for cGMPDepositorInsertGreen cGull
ExpressionMammalianPromoterCMVAvailable SinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
(133) pcDNA3.1-Nup62-Flag-EPEA
Plasmid#160744PurposeExpresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymesDepositorInsertNup62-Flag-EPEA
TagsEPEA and FlagExpressionMammalianPromoterT7/CMVAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
epB_UbC_CymRV5-nls-GFP_DEx2
Plasmid#119906PurposeePiggyBac mammalian expression plasmid for CymR-GFP fusionDepositorInsertCymRV5-nls-GFP
ExpressionMammalianAvailable SinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUW-TIE1-Cherry
Plasmid#119236PurposePlasmid encodes full-length human Tie1 gene fused in-frame with Cherry cloned in lentiviral vector.DepositorAvailable SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-Golgi
Plasmid#36205PurposeIn vivo visualization of golgi apparatus (can be used for colocalization studies)DepositorAvailable SinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a- mEGFP-kanMX6
Plasmid#105146PurposeYeast gene targetingDepositorInsertmEGFP-kanMX6
TagsmEGFPExpressionBacterial and YeastMutationA206KAvailable SinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Alpha-5-Integrin-12
Plasmid#53984PurposeLocalization: Focal Adhesions, Excitation: 487, Emission: 509DepositorAvailable SinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pdCas9-J23109
Plasmid#113151PurposePlasmid constitutively expressing dCas9 proteinDepositorInsertdCas9
ExpressionBacterialMutationD10A & H840AAvailable SinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
p11-LacY-wtx1
Plasmid#69056PurposeThe reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.DepositorAvailable SinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Halo-DNAPKcs
Plasmid#210787PurposePlasmid for expression of HaloTag-DNA-PKcs in mammalian cellsDepositorInsertDNA-PKcs
Tags3xFLAG-HaloTagExpressionMammalianPromoterCMVAvailable SinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.synP.DIO.EGFP.WPRE.hGH
Plasmid#100043PurposeAn AAV vector that expresses EGFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).DepositorHas ServiceAAV9InsertEGFP
UseAAVExpressionMammalianPromotersynapsin-1Available SinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-PPARA
Plasmid#169019PurposeExpression of PPARA driven by CMV promoterDepositorAvailable SinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUltra-H2B-mTagBFP2
Plasmid#161791Purpose3rd generation lentiviral multicistronic vector for mammalian expression of EGFP, H2B-mTagBFP2, and one gene of interestDepositorInsertH2B-mTagBFP2
UseLentiviral; MulticistronicTagsEGFP and mTagBFP2ExpressionMammalianMutationI174A in the mTagBFP2 protein according to PLoS O…PromoterUbCAvailable SinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
VEGFR2-mCh
Plasmid#108854PurposeEncodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linkerDepositorAvailable SinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-APOBEC1-YTH
Plasmid#200158PurposeFor bacterial expression of the APOBEC1-YTH fusion protein for protein purificationDepositorInsertAPOBEC1-YTH
Tags6xHis, HA, and Maltose Binding ProteinExpressionBacterialMutationCodon optimized for E coli expressionPromoterLacZAvailable SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG mito-GCaMP5G
Plasmid#105009Purposemitochondrial calcium sensorDepositorInsertGCaMP5G
ExpressionMammalianPromoterCAGAvailable SinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNMSB104
Plasmid#199314PurposeMosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR at ttTi5605DepositorInsertflp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR
ExpressionWormMutationmTagBFP2 from pJJR81, C. elegans codon optimized …Promoterflp-18Available SinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
Src-Y530F
Plasmid#124659PurposeHuman c-Src with Y530F mutationDepositorAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VSVG
Plasmid#35616DepositorInsertVSV G protein
ExpressionMammalianAvailable SinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 mCherry
Plasmid#128073Purposepuro exchanged to mCherryDepositorInsertmCherry
UseLentiviralExpressionMammalianMutationnoAvailable SinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
AviTag-SpyCatcher
Plasmid#72326PurposeBacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.DepositorInsertAviTag-SpyCatcher
TagsN-term His6 tag and TEV cleavage siteExpressionBacterialAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF1284
Plasmid#143946PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
plenti hSyn PKM1
Plasmid#220920PurposeNeuronal specific expression of PKM1DepositorAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
SuperClomeleon
Plasmid#203724PurposeAn improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chlorideDepositorInsertCerulean linked topaz fluorescent protein
ExpressionBacterial and MammalianMutationCerulean, modified Topaz Fluorescent Protein with…Available SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4-myc-His-mPGC-1a
Plasmid#24925DepositorAvailable SinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTP264
Plasmid#149334PurposeBacterial expression of Biotin ligase BirADepositorInsertBiotin ligase BirA
TagsHis14 and bdNEDD8ExpressionBacterialAvailable SinceJune 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHelper_AcCAST
Plasmid#127923PurposeExpresses AcCAST and a crRNA targeting PSP1DepositorInsertAcTnsB, AcTnsC, AcTniQ, AcCas12k
Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
P415 ymBaojin
Plasmid#224086PurposeExpressing ymBaojin in yeastDepositorInsertymBaoJin
ExpressionYeastAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
enOgeuIscB with ωRNA-v13
Plasmid#220940PurposeCMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v13 scaffold with BsmBI for guide cloingDepositorInsertsCMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
OgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning
UseCRISPRExpressionMammalianAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-cuOx144_Bxb1attB_loxP-PGKpuro-loxP
Plasmid#119903PurposeBxb1 integration plasmid for cuO array with Puro selection cassetteDepositorInsertcuOx144 array
UseMouse TargetingAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-N-Myc-SCAP
Plasmid#134285PurposeExpresses Myc-tagged SCAP in mammalian cellsDepositorAvailable SinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human Beta-catenin GFP
Plasmid#71367Purposemammalian expression of human Beta-catenin GFPDepositorAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB-EF1a-NLuc-IRES-Puro
Plasmid#130936PurposePiggyBac vector for constitutive NanoLuc expression in mammalian cellsDepositorInsertNanoLuc
ExpressionMammalianPromoterEF1aAvailable SinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSII415
Plasmid#35454DepositorTypeEmpty backboneUseYeast centromere vectorPromoterNoneAvailable SinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAL750
Plasmid#200988PurposeInducible promoter Pxyl for Haloferax volcaniiDepositorTypeEmpty backboneUseExpression in haloarchaeaPromoterPfdx, PxylAvailable SinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-Rab11-DN (S25N)
Plasmid#101046PurposeExpresses HA-tagged human Rab11-DN (S25N) in mammalian cellsDepositorAvailable SinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
DHFR-myc
Plasmid#20214DepositorAvailable SinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDEST-mCherry-LacR-BRCA1
Plasmid#71115Purposemammalian expression of mCherry-LacR-BRCA1 siRNA resistant constructDepositorAvailable SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only
