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Showing: 7051 - 7100 of 60877 results

  12. pAM4891 (pCV0003)

    Plasmid
    #120080
    Purpose
    Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a Kanamycin/Neomycin resistance gene in various cyanobacteria.
    Depositor
    Susan Golden
    Article
    Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku
    Insert
    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aph1, CV-PconII-LTRBS-GFPmut2
    Use
    Other
    Mutation
    mobA Y25F
    Available Since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120080-plasmid-map-sequence-id-234472

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  17. PB-SAM

    Plasmid
    #102559
    Purpose
    Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.
    Depositor
    Ying Liu
    Article
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Inserts
    MS2-P65-HSF1_T2A_Hygro
    dCAS9(D10A, N863A)-VP64_T2A_Blast
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9 and N55K in MS2
    Promoter
    CAG and EF1A
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102559-plasmid-map-sequence-id-193885

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  26. pHJ12: CadC-VHH-Caffeine (No linker)

    Plasmid
    #108244
    Purpose
    Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression
    Depositor
    Jerome Bonnet
    Article
    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102
    Insert
    Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
    Use
    Synthetic Biology
    Promoter
    pLacO1
    Available Since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108244-plasmid-map-sequence-id-210116

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  27. pSL0284 (pQCascade_entry)

    Plasmid
    #130635
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    130635-plasmid-map-sequence-id-255022

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  32. pSC101-Donor

    Plasmid
    #140630
    Purpose
    Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    LE, RE, KanR, temperature sensitive pSC101 origin of replication
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Available Since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    140630-plasmid-map-sequence-id-306011

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  38. pCW57-RFP-P2A-MCS (Neo)

    Plasmid
    #89182
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Expression
    Mammalian
    Promoter
    TRE
    Available Since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89182-plasmid-map-sequence-id-176305

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  43. pCW57-GFP-P2A-MCS (Neo)

    Plasmid
    #89181
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Expression
    Mammalian
    Promoter
    TRE
    Available Since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89181-plasmid-map-sequence-id-176294

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  45. pAS134

    Plasmid
    #211505
    Purpose
    AAV genome to express Venus under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq
    Depositor
    Randall Platt
    Article
    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:
    Insert
    Venus
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Expression
    Mammalian
    Available Since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    211505-plasmid-map-sequence-id-417792

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  50. pPFO*

    Plasmid
    #120400
    Purpose
    Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.
    Depositor
    Arun Radhakrishnan
    Article
    Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11
    Insert
    Mutant form of Perfringolysin O
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Amino acids 1-27 are deleted, starts at amino aci…
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120400-plasmid-map-sequence-id-233255

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Showing: 7051 - 7100 of 60877 results