We narrowed to 10,547 results for: CHL

1. pRATIO1212

   Plasmid

   #141299

   Purpose

   Gateway cloning compatible dual fluorescent ratiometric binary vector

   Depositor

   David Nelson

   Article

   Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

   Type

   Empty backbone

   Use

   Tags

   mScarlet-I and mVenus

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 3918-37

   Plasmid

   #176495

   Purpose

   pTRE-EGFP-T2A-mCherry-polyA

   Depositor

   Albert La Spada

   Article

   Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

   Insert

   Tetracyline Responsive Element driving EGFP-T2A-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBP-TL5

   Plasmid

   #72989

   Purpose

   RBS_M2

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   M2 RBS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pBP-TL11

   Plasmid

   #72995

   Purpose

   RBS_L4

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   L4 RBS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pDGE503

   Plasmid

   #153276

   Purpose

   Intermediate sgRNA array cloning vector position 1

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDGE504

   Plasmid

   #153277

   Purpose

   Intermediate sgRNA array cloning vector position 2

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGBW-m4046865

   Plasmid

   #153930

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pSBDEST.H

   Plasmid

   #79464

   Purpose

   Promoterless, Sleeping Beauty-compatible Gateway destination vector, Hygromycin selection

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Type

   Empty backbone

   Use

   Promoterless gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR-P2rP4

   Plasmid

   #141015

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MTK8b_005

    Plasmid

    #123893

    Purpose

    Encodes the 5' AAV ITR cassette as a type 8b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    5' AAV ITR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MTK3b_021

    Plasmid

    #123802

    Purpose

    Encodes VPR activation domain as a Type 3b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    VPR AD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSB3C5-proD-B0032-E0051

    Plasmid

    #107241

    Purpose

    Expresses lacZa-GFP fusion from constitutive proD promoter

    Depositor

    Joseph Davis, Robert Sauer

    Article

    Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

    Insert

    ProA-RBS(B0032)-Gemini (E0051)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    proD

    Available since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCellFree_G10

    Plasmid

    #67144

    Purpose

    Gateway destination vector for cell-free expression with C-term sfGFP 8xHis tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    HIS and sfGFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-CaMKIIa-hChR2(C128A)-EYFP-WPRE

    Plasmid

    #20293

    Purpose

    Lentiviral expression of ChR2 (C128A) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

    Depositor

    Karl Deisseroth

    Article

    Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 128 to Alanine

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pReporter_3

    Plasmid

    #60564

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSB_init-Sb66

    Plasmid

    #159422

    Purpose

    Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb66

    Depositor

    Dianfan Li

    Article

    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

    Insert

    Sb66
    

    Use

    Tags

    c-Myc and poly-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTrc_AraC-I113-LOV

    Plasmid

    #206804

    Purpose

    Expresses AraC-I113-LOV from a Trc promoter. For use in E. coli

    Depositor

    Dominik Niopek

    Article

    Mathony et al Adv Sci (Weinh). 2023 Oct;10(28):e230349

    Insert

    AraC-AsLOV2 fusion
    

    Use

    Synthetic Biology

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTrc_AraC-S170-LOV

    Plasmid

    #206805

    Purpose

    Expresses AraC-S170-LOV from a Trc promoter. For use in E. coli

    Depositor

    Dominik Niopek

    Article

    Mathony et al Adv Sci (Weinh). 2023 Oct;10(28):e230349

    Insert

    AraC-AsLOV2 fusion
    

    Use

    Synthetic Biology

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CDS12_mTurquoise2

    Plasmid

    #136089

    Purpose

    FP for N-term fusion with a CTAG

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CDS12_mTurquoise2(noStop)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. TDGS50

    Plasmid

    #183189

    Purpose

    TEV protease expressed under J23106 promoter; DivIVAsfGFP-TEV protease recognition site-M0050 expressed under J23116 promoter

    Depositor

    Hsiao-Chun Huang

    Article

    Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

    Inserts

    DivIVA-GFP(tevR M0050)
    TEV-S219V
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CDS_LUC

    Plasmid

    #136083

    Purpose

    Firefly luciferase

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CDS_LUC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pASKt15C+SrtA

    Plasmid

    #64983

    Purpose

    Expresses the wild type of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    His6 tag and S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59

    Promoter

    tetracycline promoter/operator

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDEST-APIC-Cas9

    Plasmid

    #121657

    Purpose

    gateway cloning destination vector for expressing enhancer driven Cas9

    Depositor

    Chun Han

    Article

    Poe et al Genetics. 2018 Nov 30. pii: genetics.118

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGL2_174

    Plasmid

    #199106

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Charlie Gilbert, Cultivarium Tools, Nili Ostrov

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pNG2 ORI plus NGS barcode 22, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBTK150

    Plasmid

    #183126

    Purpose

    BTK Type 2 promoter part for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al Science. 2020 Jan 31;367(6477):573-576.

    Insert

    Reverse rnpB-T1 terminator and forward CP25 promoter for dsRNA expression
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pGL2_177

    Plasmid

    #199109

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Charlie Gilbert, Cultivarium Tools, Nili Ostrov

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pBC1 ORI plus NGS barcode 25, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pATD

    Plasmid

    #141012

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    HSV-TK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p15aC-4D1D

    Plasmid

    #107864

    Purpose

    Bacterial expression of PIS and PI4K

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Inserts

    PIS
    phosphatidylinositol 4-kinase β
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    Promoter

    proD

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBP-TL4

    Plasmid

    #72988

    Purpose

    RBS_H5

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    H5 RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pBP-TL8

    Plasmid

    #72992

    Purpose

    RBS_L1

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    L1 RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

31. pBP-L1U1H09

    Plasmid

    #73008

    Purpose

    L1U1H09 terminator

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    L1U1H09 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMINTC3GH (Apr)

    Plasmid

    #110078

    Purpose

    Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDEST-APIC-LexAGAD

    Plasmid

    #112807

    Purpose

    gateway cloning destination vector to express enhancer driven LexAGAD in Drosophila

    Depositor

    Chun Han

    Article

    Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pReporter_9

    Plasmid

    #60569

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pReporter_4

    Plasmid

    #60565

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGP4G-TPLRD1-ccdB

    Plasmid

    #83877

    Purpose

    Destination vector for integration at yeast Trp locus with N-terminal TPL repression domain (RD) for auxin response assays

    Depositor

    Jennifer Nemhauser

    Article

    Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. MTK2_006

    Plasmid

    #123701

    Purpose

    Encodes pSV40 as a Type 2 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pSV40
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBP-Bba_K822000

    Plasmid

    #74094

    Purpose

    L-Lactate inducible promoter from E. coli MG1655

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Bba_K822000 (L-Lactate promoter - E. coli MG1655)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    NA

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCG-A-sacB(SP)-B

    Plasmid

    #87370

    Purpose

    An ORF part-containing vector harbouring the signal peptide from SacB with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

    Depositor

    Tom Ellis

    Article

    Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

    Insert

    SacB signal peptide
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAK02

    Plasmid

    #19001

    Depositor

    Jay Keasling

    Article

    Khlebnikov et al J Bacteriol. 2000 Dec . 182(24):7029-34.

    Insert

    arabinose transporter araE under control of Ptac (araE E. coli)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

41. pAAV_TALEN-FokI(WT)-BB_V3

    Plasmid

    #42588

    Depositor

    Feng Zhang

    Article

    Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

    Insert

    TALEN-FokI(WT)-BB
    

    Use

    AAV; Adeno-associated virus ()

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG hybrid

    Available since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pMEXC3GH (Apr)

    Plasmid

    #110083

    Purpose

    Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLY185

    Plasmid

    #184146

    Purpose

    RFP generator controlled by Plux2 promoter

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    rfp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Plux2

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pReporter_11

    Plasmid

    #60571

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_11
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pJB057

    Plasmid

    #98911

    Purpose

    Express Dronpa tagged ZapA in E coli

    Depositor

    Jie Xiao

    Article

    Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

    Insert

    ZapA
    

    Use

    Tags

    Dronpa

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCFJ1656

    Plasmid

    #51483

    Purpose

    miniMos transposable element with HygroR and peel-1, R4-R3 Gateway cloning

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCellFree_G05

    Plasmid

    #67139

    Purpose

    Gateway destination vector for cell-free expression with N-term 8xHis mCherry tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    His and mCherry

    Expression

    Mutation

    Promoter

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

48. pCG-D-SpyTag-His6-E

    Plasmid

    #87375

    Purpose

    An ORF part-containing vector harbouring the SpyTag sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF assembly

    Depositor

    Tom Ellis

    Article

    Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

    Insert

    SpyTag
    

    Use

    Synthetic Biology

    Tags

    His6 tag

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. pLenti-CaMKIIa-hChR2(C128T)-EYFP-WPRE

    Plasmid

    #20295

    Purpose

    Lentiviral expression of ChR2 (C128T) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

    Depositor

    Karl Deisseroth

    Article

    Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 128 to Threonine

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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50. pT7_circRAJ31_C873U

    Plasmid

    #69932

    Purpose

    Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant is deficient in circularisation. Under control of a T7 promoter.

    Depositor

    Alfonso Jaramillo

    Article

    Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

    Insert

    pT7_circRAJ31_C873U
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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