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169,365 results

Showing: 7201 - 7250 of 169365 results

  1. ppyCAG_RNaseH1_WKKD

    Plasmid
    #111905
    Purpose
    Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids
    Depositor
    Xiang-Dong Fu
    Article
    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.
    Insert
    RNASEH1 (RNASEH1 Human)
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…
    Available Since
    July 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    111905-plasmid-map-sequence-id-212872

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  5. pHR SFFVp MCP-mCherry IRES h2b-diRFP

    Plasmid
    #102351
    Purpose
    Lentiral expression vector bearing the MS2 Coat Protein (MCP) fused to mCherry as well as the nuclear marker histone 2b fused to two copies of irFP. Also bears the hygromycin selection marker.
    Depositor
    Jared Toettcher
    Article
    Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d
    Insert
    MS2 Coat Protein
    Use
    Lentiviral
    Tags
    mCherry
    Promoter
    SFFV
    Available Since
    Jan. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    102351-plasmid-map-sequence-id-197294

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  6. prhaBAD-pA-Hia5

    Plasmid
    #222303
    Purpose
    Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    pA-Hia5
    Tags
    6His and protein A
    Expression
    Bacterial
    Mutation
    codon optimized for bacterial expression
    Promoter
    rhaBAD
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222303-plasmid-map-sequence-id-447746

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  20. pCBC-DT2T3

    Plasmid
    #50591
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T2, gRNA scaffold, U6-29t plus, U6-1p, T3
    Use
    CRISPR; Pcr template
    Expression
    Plant
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50591-plasmid-map-sequence-id-244476

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  22. pT7TS-rich

    Plasmid
    #99050
    Purpose
    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS. More sites for cloning are incorporated.
    Depositor
    Richard Bélanger
    Type
    Empty backbone
    Use
    Unspecified; Xenopus oocyte system
    Promoter
    pT7
    Available Since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    99050-plasmid-map-sequence-id-191760

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  23. pFC331

    Plasmid
    #87843
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and argB selection marker
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    argB
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and native pro…
    Available Since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87843-plasmid-map-sequence-id-177947

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  31. pDS-Red1-N1 AMPA GluR-B(flip)

    Plasmid
    #109142
    Purpose
    The expression vector carries the red fluorescent protein tagged at the C-terminus of GluA2 subunit flip spliced form of the AMPA receptor.
    Depositor
    Stefano Vicini
    Insert
    AMPA receptor
    Tags
    RFP
    Expression
    Mammalian
    Mutation
    Glua2 subunit flip spliced form of the AMPA recep…
    Promoter
    CMV
    Available Since
    June 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    109142-plasmid-map-sequence-id-209556

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  41. pCAG-GFP-PQRv3-RFP

    Plasmid
    #73951
    Purpose
    Protein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.
    Depositor
    Brian Chen
    Article
    Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do
    Inserts
    sfGFP (superfolder GFP)
    RFP
    Tags
    A residual PQR peptide wii be left at the C-termi…
    Expression
    Mammalian
    Promoter
    Chicken beta actin and Chicken beta actin (shared…
    Available Since
    Jan. 6, 2017
    Availability
    Academic Institutions and Nonprofits only

  45. pLEW100v5-HYG

    Plasmid
    #24012
    Depositor
    George Cross
    Insert
    Luciferase
    Use
    Trypanosome expression
    Available Since
    May 11, 2010
    Availability
    Academic Institutions and Nonprofits only
    24012-plasmid-map-sequence-id-11283

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  47. FU-H2B-GFP-IRES-Puro

    Plasmid
    #69550
    Purpose
    Lentiviral, selectable fluorescent GFP tagging of nuclei.
    Depositor
    Charles Gersbach
    Insert
    Histone 2B - green fluorescent protein fusion
    Use
    Lentiviral
    Tags
    GFP fusion
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69550-plasmid-map-sequence-id-122943

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  49. TFORF2638

    Plasmid
    #142344
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TFDP1 (TFDP1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    142344-plasmid-map-sequence-id-396961

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Showing: 7201 - 7250 of 169365 results