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173,197 results

Showing: 7201 - 7250 of 173197 results

  26. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100901-plasmid-map-sequence-id-197197

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  29. AbVec_IGHC

    Plasmid
    #183701
    Purpose
    Encode human IgG1 heavy chain constant region. For cloning variable region and mAb expression.
    Depositor
    David Nemazee
    Insert
    IGHG1 (IGHG1 Human)
    Expression
    Mammalian
    Promoter
    SP6
    Available Since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    183701-plasmid-map-sequence-id-358532

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  35. pAAV.GfaABC1D.GluSnFr.SV40

    Plasmid
    #100889
    Purpose
    AAV expression of GluSnFR, a glutamate sensor
    Depositor
    Baljit Khakh
    Insert
    GluSnFr
    Use
    AAV
    Expression
    Mammalian
    Promoter
    GfaABC1D
    Available Since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    100889-plasmid-map-sequence-id-199083

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  41. pZA16mflon

    Plasmid
    #75439
    Purpose
    Plasmid for arabinose-induced expression of mf-lon. Note that this plasmid contains the original mf-lon cassette that is not codon optimized for expression in E. coli.
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    mf-lon
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75439-plasmid-map-sequence-id-147407

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  42. Gs(short)-CASE

    Plasmid
    #168124
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAS, GNB1, GNG1 (GNGT1, GNAS, GNB1 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG1
    Expression
    Mammalian
    Mutation
    NLuc is inserted at N136/V137 within GNAS
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168124-plasmid-map-sequence-id-332868

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  47. AbVec_IGKC

    Plasmid
    #183702
    Purpose
    Encode human Ig kappa chain constant region. For cloning variable region and mAb expression.
    Depositor
    David Nemazee
    Insert
    IGKC (IGKC )
    Expression
    Mammalian
    Promoter
    SP6
    Available Since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    183702-plasmid-map-sequence-id-358531

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Showing: 7201 - 7250 of 173197 results