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Plasmid#26871PurposeMammalian expression of cytoplasmic CRY2-mCherryDepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationNLS sequences deletedPromoterCMVAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
NLS-4LNDD-pcDNA3.1(+)
Plasmid#172313PurposeExpresses ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS) in mammalian cellsDepositorInsertLambda N peptide - human ADAR2 Deaminase Domain
UseTags6xHis, Flag, and Nuclear Localization Signal of S…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRvUBC-mEGFP-NLS
Plasmid#205010PurposeThe vector contains 1kbp of the upstream and downstream regions of the ubiquitin c gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the ubiquitin c gene from R. varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Myc:P4:gs:nls:ABI
Plasmid#135985PurposeExpresses the P4 coiled coil fused to the ABI abscisic acid-inducible domain in mammalian cellsDepositorInsertP4 coiled-coil fused to the ABI domain
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-UltraCas12a-6xNLS
Plasmid#209023PurposeLentiviral vector expressing human codon-optimized UltraCas12a with 6xNLSDepositorInsertUltraCas12a-6xNLS-Myc-NeoR
UseCRISPR and LentiviralTagsMYCExpressionMammalianMutationM537R/F870LPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
459 p3E-2A-nlsEGFPpA
Plasmid#195969PurposeGateway p3E 2A fluorophoreDepositorInsert2A-nlsEGFP (nuclear)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-NLS-ZapCV2-ALA
Plasmid#203665PurposeExpresses zinc dead sensor ZapCV2-Ala in the nucleus of mammalian cellsDepositorInsertZapCV2-Ala zinc dead sensor
UseTagsSV40 NLSExpressionMammalianMutationCys and His in Zap1 is mutated with AlaPromoterCMV promoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
NM9-SpCas9-NLS3
Plasmid#128177PurposeExpresses R. toruloides codon-optimized SpCas9 under PGK1 promoterDepositorInsertSpCas9-NLS3
UseCRISPRTagsExpressionYeastMutationPromoterpPGK1Available sinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQC NLS mCherry IX
Plasmid#37354DepositorInsertNLS-mCherry
UseTagsExpressionMutationPromoterAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti-opCas12a-6xNLS
Plasmid#209022PurposeLentiviral vector expressing human codon-optimized OpCas12a with 6xNLSDepositorInsertopCas12a-6xNLS-Flag-Myc-NeoR
UseCRISPR and LentiviralTagsFLAG and MYCExpressionMammalianMutationE174R/S542RPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-MCP-3XBFPnls
Plasmid#75383PurposeMCP-3XBFPnlsDepositorInsertMCP
UseLentiviralTags3XBFPExpressionMammalianMutationPromoterCMV-TOAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFUGW NLS-FlpO
Plasmid#183448PurposeLentiviral FlpO vectorDepositorInsertNLS-FlpO
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAd-PV-NLS-DsRed
Plasmid#17287DepositorInsertPV-DsRed-NLS-CD (Pvalb Rat)
UseAdenoviralTagsMycExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEF-APEX2-NLS
Plasmid#140281PurposeExpression of APEX2-NLSDepositorInsertAPEX2-NLS
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
H_NLS 41kDa (BFP)
Plasmid#201391PurposeNucleocytoplasmic transport probeDepositorInsert(NLS)SV40-BFP-2PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-PCNA-19-SV40NLS-4
Plasmid#55250PurposeLocalization: Nucleus, Excitation: 383, Emission: 448DepositorInsertPCNA-19 SV40NLS (PCNA Human)
UseTags3xSV40 NLS and EBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSL1180-HR-PUbEBFP2-NLS
Plasmid#176682PurposeExpression of nuclear localized EBFP2 under Ae. aegypti Polyubiquitin promoter (AAEL003888), EBFP2 version of pSL1180-HR-PUbECFP (Addgene #47917)DepositorInsertEBFP2
UseTagsExpressionInsectMutationPromoterAe. aegypti poly-ubiquitin (AAEL003888)Available sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-NLS-M-MLV-RT
Plasmid#181804PurposepET28a-NLS-M-MLV RTDepositorInsertNLS-M-MLV-RT
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-SpCas9mSA-His
Plasmid#182951PurposeExpress SpCas9mSA in bacteriaDepositorInsertNLS-SpCas9mSA-His
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICE-NLS-mCherry
Plasmid#60364PurposePlasmid for expression of NLS-mCherry (control plasmid). Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorInsertNLS-mCherry
UseTagsNLS from SV40 T antigen and mCherryExpressionMutationPromoterCMV-tetAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCIB1(deltaNLS)-pmGFP
Plasmid#28240PurposeExpresses CIB1 fused to plasma membrane-localized EGFPDepositorInsertCIB1(deltaNLS)-pmGFP (CIB1 Mustard Weed)
UseTagsEGFPExpressionMammalianMutationNLS sequences mutatedPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBCCP-GFP-NLS
Plasmid#173923PurposeFluorescent labeling of the nuclear envelopeDepositorInsertBiotin carboxyl carrier protein, AcGFP1
UseTags3xNLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
spCas9-NLS-MAV
Plasmid#124212PurposeSpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)DepositorInsertcas9
UseCRISPRTagsHis6, MBP, TEV Cleavage site, and two copies of S…ExpressionBacterialMutationPromoterT7Available sinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
NLS-dCas9-trCIB1
Plasmid#64119PurposePhotoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1DepositorInsertdCas9-trCIB1 fusion
UseTagsHA and NLSExpressionMammalianMutationdCas9 (D10A H840A, catalytically inactive), trCIB…PromoterCMVAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ICUE3-NLS
Plasmid#138218PurposeNuclear-targeted FRET-based cAMP indicator.DepositorInsertICUE3-NLS
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
dCas9-AsLOV2x3-NLSx3
Plasmid#231566PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertdCas9-AsLOV2x3
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRvTUB-mEGFP-NLS
Plasmid#205011PurposeThe vector contains 1kbp of the upstream and downstream regions of the tubulin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the tubulin gene from R. varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLSx3-CRY2PHR-p65
Plasmid#64124PurposePhotoactivatable transcription system. Expression of activator probe, which includes the photolyase homology region of CRY2 (CRY2PHR) and the transcriptional activator domain.DepositorInsertCRY2PHR-p65
UseTagsHis, NLSx3, and V5ExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
NLS-LucWT-GFP
Plasmid#127709PurposeThe expressed ORF encodes nucleus location signal with GFP and WT Luciferase.DepositorInsertLuciferase
UseTagsEGFP and FLAG-NLSExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-QUAS5x:GFPNLS-SV40pA
Plasmid#155122PurposeTol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertQUAS5x:GFPNLS
UseZebrafish expressionTagsExpressionMutationPromoterQUAS5x-E1bAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_N-DYK_MTHFD1-NLS
Plasmid#133023PurposeNLS sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)DepositorInsertMTHFD1 (MTHFD1 Human)
UseTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Prab-3::NLS::GFP
Plasmid#68120PurposeNuclear Localized GFP for pan-neuronal expression in C. ElegansDepositorInsertGFP
UseTagsExpressionWormMutationPromoterrab-3Available sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
GANES-DEVD-BNLS
Plasmid#50842PurposeExpresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS.DepositorInsertddGFP A and ddGFP B
UseTagsNES sequence LALKLAGLDIGS placed after ddGFP A an…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
epB_CAG_TetRFlag-nls-tdTom_DEx4
Plasmid#119909PurposeePiggyBac mammalian expression plasmid for TetR-tdTom fusionDepositorInsertTetRFlag-nls-tdTom
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Prab-3::NLS::GCaMP6s
Plasmid#68119PurposeNuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. ElegansDepositorInsertrab-3::NLS::GCaMP6s
UseTagsExpressionWormMutationPromoterrab-3Available sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPB-3xnls-Tq-Ca-FLITS
Plasmid#145030PurposepiggyBac vector for expressing a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsert3xnls-Tq-Ca-FLITS
UseTags3xnls-Tq-Ca-FLITSExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLBS.EF NLS-pHlu
Plasmid#129324PurposepH measurement, nucleus, lentivirusDepositorInsertpHluorin (from H. Imamura)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ EGFP-3NLS
Plasmid#165400PurposeExpresses nuclear localized EGFPDepositorInsertNuclear Localization Signal
UseAvian, xenopus, zebrafishTagsEGFPExpressionMammalianMutationPromoterSP6Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+_NLS-Tir1-HA
Plasmid#117716PurposemRNA synthesis of NLS-TIR1-HA for injectionDepositorInsertTRANSPORT INHIBITOR RESPONSE 1
UseTagsHA and NLSExpressionMammalianMutationcodon-optimized TIR1 for murine expressionPromoterSP6Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR-NLS
Plasmid#64931PurposeNuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.DepositorInsertTORCAR-NLS (EIF4EBP1 Human, Synthetic)
UseTagsCerulean, Nuclear localization signal (NLS), and …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only