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19,186 results

Showing: 7801 - 7850 of 19186 results

  3. pDR121

    Plasmid
    #37148
    Depositor
    Dale Ramsden
    Insert
    XRCC6 (XRCC6 Human)
    Use
    Tags
    H6
    Expression
    Insect
    Mutation
    Promoter
    Available since
    July 18, 2012
    Availability
    Academic Institutions and Nonprofits only

  8. pcDNA3.3_SARS2_XBB.1.5

    Plasmid
    #196585
    Purpose
    expressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (XBB.1.5)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G252V, F486P on the basis of XBB
    Promoter
    CMV
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196585-plasmid-map-sequence-id-386779

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  14. pDF0114 pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site

    Plasmid
    #172508
    Purpose
    Encodes the mature DisCas7-11 DR along with a golden gate site for spacer cloning
    Depositor
    Jonathan Gootenberg, Omar Abudayyeh
    Article
    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-
    Insert
    pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    172508-plasmid-map-sequence-id-344931

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  15. AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

    Plasmid
    #65454
    Purpose
    Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinases
    Depositor
    Hongkui Zeng
    Article
    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10
    Insert
    ChR2(H134R)-EYFP
    Use
    AAV
    Tags
    EYFP
    Expression
    Mutation
    H134R variant
    Promoter
    hSyn1
    Available since
    July 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    65454-plasmid-map-sequence-id-436383

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  17. pSFVCs-lacZ

    Plasmid
    #92076
    Purpose
    This is a high level expression plasmid for production of recombinant genomic SFV RNA encoding a C-beta galactosidase fusion protein.
    Depositor
    Henrik Garoff
    Article
    Sjoberg et al Biotechnology (N Y). 1994 Nov;12(11):112
    Insert
    C-lacZ
    Use
    C-lacz expression plasmid, for run-off transcript…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92076-plasmid-map-sequence-id-181625

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  21. pmSTARRseq1

    Plasmid
    #96945
    Purpose
    CpG free plasmid for testing effects of methylation on regulatory activity
    Depositor
    Jenny Tung
    Type
    Empty backbone
    Use
    Mstarr-seq screening vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CpG -free EF1 promoter
    Available since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    96945-plasmid-map-sequence-id-191112

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  22. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid
    #91249
    Purpose
    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.
    Inserts
    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    91249-plasmid-map-sequence-id-181276

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  23. pOpen-ETSSB

    Plasmid
    #165549
    Purpose
    ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    Extreme Thermostable Single-Stranded DNA Binding Protein
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165549-plasmid-map-sequence-id-339430

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  24. pAAV-hSyn-hChR2(H134R)-mCherry

    Plasmid
    #26976
    Purpose
    AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation
    Depositor
    Karl Deisseroth
    Has Service
    AAV5, AAV8, and AAV9
    Insert
    hChR2(H134R)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    hSyn
    Available since
    Feb. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    26976-plasmid-map-sequence-id-313556

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  28. pGEX 6P1 CDK2 (552)

    Plasmid
    #61845
    Purpose
    bacterial expression of GST CDK2 WT
    Depositor
    Jonathon Pines
    Insert
    CDK2 (CDK2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  29. p2attNG-H11-short

    Plasmid
    #51546
    Purpose
    p2attNG landing pad plasmid with 400 bp homology arms to the human H11 locus (for use with TALEN-assisted recombination).
    Depositor
    Michele Calos
    Article
    Zhu et al Nucleic Acids Res. 2013 Dec 4.
    Insert
    GFP4m
    Use
    Human targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51546-plasmid-map-sequence-id-78774

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  31. pDR123

    Plasmid
    #37149
    Depositor
    Dale Ramsden
    Insert
    XRCC5 (XRCC5 Human)
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    July 18, 2012
    Availability
    Academic Institutions and Nonprofits only

  33. p406TEF1

    Plasmid
    #15976
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    TEF1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15976-plasmid-map-sequence-id-8461

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  34. pLV-EBFP2-nuc

    Plasmid
    #36085
    Depositor
    Pantelis Tsoulfas
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Replace EYFP of the vector pEYFP-Nuc from Clontec…
    Promoter
    Available since
    June 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    36085-plasmid-map-sequence-id-44618

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  43. hGli1 flag3x

    Plasmid
    #84922
    Purpose
    mammalian expression of Gli1
    Depositor
    Martin Fernandez-Zapico
    Insert
    Gli1 (GLI1 Human)
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84922-plasmid-map-sequence-id-422144

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  48. mCherry-Gamma-Tubulin-17

    Plasmid
    #55050
    Purpose
    Localization: Centrosomes, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Gamma-Tubulin (TUBG1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55050-plasmid-map-sequence-id-215600

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  49. CMV-GEM-GECO1

    Plasmid
    #32442
    Purpose
    Mammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    GEM-GECO1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32442-plasmid-map-sequence-id-117095

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  50. pEGFP-centrin-1

    Plasmid
    #72641
    Purpose
    The plasmid encodes centrin-1 with an N-terminal EGFP tag
    Depositor
    Michel Bornens
    Insert
    centrin-1 (CETN1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72641-plasmid-map-sequence-id-132790

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Showing: 7801 - 7850 of 19186 results