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162,408 results

Showing: 8501 - 8550 of 162408 results

  2. TIRAP-GFP

    Plasmid
    #52739
    Purpose
    Retroviral vector expresses WT hTIRAPfused to GFP. Also contains IRES-hCD2 as selectable marker
    Depositor
    Jonathan Kagan
    Article
    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10
    Insert
    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 20, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. Non tagged Avidin

    Plasmid
    #214835
    Purpose
    This plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
    Depositor
    Hironori Funabiki
    Article
    Arimura et al bioRxiv 2024.01.21.576499.
    Insert
    Non tagged Avidin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    214835-plasmid-map-sequence-id-423116

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  7. CHRM5-Tango

    Plasmid
    #66252
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    CHRM5 (CHRM5 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pAAV hPGRN

    Plasmid
    #213682
    Purpose
    Expresses human progranulin (PGRN) with an N-terminal twin-Strep-V5 tag
    Depositor
    Thomas Kukar
    Article
    Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi
    Insert
    Human Progranulin (hPGRN) (GRN Human)
    Use
    AAV
    Tags
    Twin-Strep tag and V5 tag (after signal peptide)
    Expression
    Mutation
    Promoter
    cytomegalovirus enhancer/chicken β-actin promoter
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    213682-plasmid-map-sequence-id-455751

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  11. pFC332

    Plasmid
    #87845
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    hph (hygromycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87845-plasmid-map-sequence-id-418925

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  13. TFORF3007

    Plasmid
    #144483
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SMAD1 (SMAD1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    144483-plasmid-map-sequence-id-407358

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  22. pPEE7

    Plasmid
    #128357
    Purpose
    Recycable marker for use in Cryptococcus neoformans. Contains the amdS1 counterselectable marker flanked by direct repeats.
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Type
    Empty backbone
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    ACT1
    Available since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    128357-plasmid-map-sequence-id-254608

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  23. AbVec2.0-IGHG4

    Plasmid
    #99578
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG4 isotype
    Depositor
    Hedda Wardemann
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV IE1 gene promoter
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    99578-plasmid-map-sequence-id-192595

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  27. pRPF215

    Plasmid
    #106377
    Purpose
    C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon
    Depositor
    Robert Fagan, Neil Fairweather
    Article
    Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1
    Insert
    Ptet-himar1-Ter(slpA)-transposon
    Use
    E. coli - c. difficile shuttle vector
    Tags
    Expression
    Mutation
    Codon optimised for C. difficile
    Promoter
    Ptet
    Available since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    106377-plasmid-map-sequence-id-205135

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  28. pBabe-CD44v4

    Plasmid
    #107969
    Purpose
    expression of CD44 variant
    Depositor
    Georg Weber
    Insert
    mCD44v4 (Cd44 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    splice variant; see Depositor Comments below
    Promoter
    Available since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    107969-plasmid-map-sequence-id-222222

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  29. pJYScr

    Plasmid
    #132719
    Purpose
    Plasmid for an easy-to-construct crRNA delivery vector using hybridized oligonucleotides. Constitutive transcription of crRNA, Spectinomycin resistance
    Depositor
    Jan Marienhagen
    Article
    Krumbach et al ACS Synth Biol. 2019 Dec 11. doi: 10.102
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    132719-plasmid-map-sequence-id-259037

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  31. TFORF1479

    Plasmid
    #143504
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TP63 (TP63 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143504-plasmid-map-sequence-id-400403

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  34. pIVM_02

    Plasmid
    #204500
    Purpose
    Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)
    Depositor
    Alessio Ciulli
    Article
    Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.
    Insert
    amino acids 54-213 VHL (VHL Human)
    Use
    Tags
    6xHis, TEV, and Thrombin
    Expression
    Bacterial
    Mutation
    0
    Promoter
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204500-plasmid-map-sequence-id-406419

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  38. TFORF2918

    Plasmid
    #142172
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFIB (NFIB Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142172-plasmid-map-sequence-id-397711

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  39. p189

    Plasmid
    #65207
    Purpose
    Mutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.
    Depositor
    LuZhe Sun
    Article
    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d
    Insert
    EGFP
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Premature stop codon added to pGEM5Z(+)-EGFP (TGG…
    Promoter
    CMV
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  44. pGEM5Z(+)-EGFP

    Plasmid
    #65206
    Purpose
    EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.
    Depositor
    LuZhe Sun
    Article
    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d
    Insert
    EGFP
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Restriction enzyme recognition sites added either…
    Promoter
    CMV
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  45. pRYG

    Plasmid
    #113642
    Purpose
    Positive control: shows the ratio of GFP signal to RFP signal with a tyrosine residue in the linker that connects the two genes.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    mRFP1-linker-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    113642-plasmid-map-sequence-id-214560

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  46. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q66

    Plasmid
    #111735
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q66 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    111735-plasmid-map-sequence-id-226804

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Showing: 8501 - 8550 of 162408 results