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175,587 results

Showing: 8601 - 8650 of 175587 results

  6. pDule-tfmF A65V S158A

    Plasmid
    #85484
    Purpose
    Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.
    Insert
    tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32V F108W Q109M I159M with 65Val and 158Ala
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85484-plasmid-map-sequence-id-169864

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  9. hGli3 flag3x

    Plasmid
    #84921
    Purpose
    mammalian expression of Gli3
    Depositor
    Martin Fernandez-Zapico
    Insert
    Gli3 (GLI3 Human)
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84921-plasmid-map-sequence-id-421628

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  19. pKHR5

    Plasmid
    #74593
    Purpose
    an alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.
    Depositor
    David Grunwald
    Article
    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:
    Insert
    FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
    Use
    Cre/Lox; Zebrafish expression
    Promoter
    alpha-crystallin
    Available Since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    74593-plasmid-map-sequence-id-140738

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  20. pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73022
    Purpose
    Luminescent and fluorescent dual reporter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1
    Available Since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73022-plasmid-map-sequence-id-136024

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  23. pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)

    Plasmid
    #64217
    Purpose
    Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A
    Depositor
    Ralf Kuehn
    Article
    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103
    Inserts
    Cas9
    hU6 promoter; BbsI sites for sgRNA
    H1 promoter; BamHI site for shRNA
    Use
    CRISPR
    Tags
    3xFLAG, NLS, and T2A-mCherry
    Expression
    Mammalian
    Promoter
    CBh, H1, and U6
    Available Since
    Sept. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    64217-plasmid-map-sequence-id-123057

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  25. pT7-V5-SBP-C1-HshnRNPM

    Plasmid
    #64924
    Purpose
    Expresses V5- and SBP-tagged hnRNPM in mammalian cells
    Depositor
    Elisa Izaurralde
    Insert
    hnRNPM (HNRNPM Human)
    Tags
    V5 and SBP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    64924-plasmid-map-sequence-id-109670

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  26. GFP-ESR1

    Plasmid
    #65211
    Purpose
    Full length mammalian expression vector for hESR1
    Depositor
    Ratna Vadlamudi
    Insert
    esr1 (ESR1 Human)
    Tags
    AcGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    65211-plasmid-map-sequence-id-422716

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  29. pEGFP rbFOX1

    Plasmid
    #63085
    Purpose
    Mammalian expression of EGFP tagged FOX1
    Depositor
    Nicolas Charlet-Berguerand
    Insert
    RBFOX1 (RBFOX1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63085-plasmid-map-sequence-id-104484

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  35. pSBbi-RH

    Plasmid
    #60516
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60516-plasmid-map-sequence-id-97530

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  46. CAG-Flex-TC66T

    Plasmid
    #48331
    Purpose
    Cre-dependant TVA receptor-mCherry fusion with a mutation (66T) in TVA
    Depositor
    Liqun Luo
    Article
    Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 1
    Insert
    TVA receptor mCherry fusion with the 66T mutation
    Use
    AAV and Cre/Lox
    Tags
    mCherry fusion to TVA receptor
    Expression
    Mammalian
    Mutation
    66T
    Promoter
    CAG
    Available Since
    Dec. 6, 2013
    Availability
    Academic Institutions and Nonprofits only
    48331-plasmid-map-sequence-id-130617

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Showing: 8601 - 8650 of 175587 results