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19,190 results

Showing: 8601 - 8650 of 19190 results

  2. pIVTRup

    Plasmid
    #101362
    Purpose
    Highly efficient in vitro transcription. Empty backbone.
    Depositor
    Ángel Raya
    Insert
    5'UTR-EcoRV site-3'UTR-pAtrack(55 pA)
    Use
    For cloning a gene for in vitro transcription pur…
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    101362-plasmid-map-sequence-id-352564

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  7. N-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58693
    Purpose
    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    3xFlag, GyrA Nsplit Intein, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58693-plasmid-map-sequence-id-90684

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  12. pN3-Nipbl

    Plasmid
    #107716
    Purpose
    Expression plasmid for 3xFLAG-tagged NIPBL
    Depositor
    Guntram Suske
    Insert
    NIPBL (Nipbl Mouse)
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    107716-plasmid-map-sequence-id-307684

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  16. mCherry-WASH1-C-18

    Plasmid
    #55162
    Purpose
    Localization: Cytoskeleton, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    WASH1
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pMXS-IRES-Hygro

    Plasmid
    #164142
    Purpose
    Retroviral expression vector with hygromycin resistance
    Depositor
    Richard Possemato
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    164142-plasmid-map-sequence-id-325860

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  23. pVITRO1-dV-IgG1/λ

    Plasmid
    #52214
    Purpose
    Human immunoglobulin gamma 1 antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin gamma 1 constant region
    Human immunoglobulin lambda constant region
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    52214-plasmid-map-sequence-id-432867

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  25. BAP1 C91A

    Plasmid
    #81025
    Purpose
    Retroviral vector containing human BAP1 C91A (catalytically inactive mutant) and Thy1.1 selection marker
    Depositor
    Anjana Rao
    Article
    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.
    Insert
    BAP1 (BAP1 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    BAP1 C91A (point mutation, catalytically inactive)
    Promoter
    5’LTR
    Available since
    Nov. 17, 2016
    Availability
    Academic Institutions and Nonprofits only

  26. CREBBP

    Plasmid
    #38977
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CREBBP (CREBBP Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  27. pSicoR-Ef1a-mCh

    Plasmid
    #31847
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.
    Depositor
    Bruce Conklin
    Article
    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for shRNA; Ef1alpha for mCherry
    Available since
    Jan. 3, 2012
    Availability
    Academic Institutions and Nonprofits only
    31847-plasmid-map-sequence-id-35883

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  29. pDestTol2pA2-U6:gRNA

    Plasmid
    #63157
    Purpose
    Destination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.
    Depositor
    Leonard Zon
    Article
    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15
    Type
    Empty backbone
    Use
    CRISPR; Tol2 destination vector for gateway
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    63157-plasmid-map-sequence-id-200862

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  30. pDONR207 SARS-CoV-2 NSP1

    Plasmid
    #141255
    Purpose
    Gateway-compatible Entry vector, with insert of NSP1 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    NSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    April 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141255-plasmid-map-sequence-id-288043

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  38. CAG-GFP/cre

    Plasmid
    #49054
    Purpose
    Retroviral vector contains CAG promoter , CMV enhancer and a large synthetic intron that used to allow the ubiquitous expression of GFP-fused Cre recombinase
    Depositor
    Fred Gage
    Article
    Tashiro et al Nat Protoc. 2006;1(6):3049-55.
    Insert
    GFP/Cre
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    49054-plasmid-map-sequence-id-72065

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  44. pMSP12::mCherry

    Plasmid
    #30169
    Depositor
    Lalita Ramakrishnan
    Insert
    Mycobacterium Strong Promoter (MSP)
    Use
    Mycobacteria expression
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    June 3, 2011
    Availability
    Academic Institutions and Nonprofits only
    30169-plasmid-map-sequence-id-214699

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  46. CoV2-N-P199L

    Plasmid
    #177949
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P199L). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (P199L) (N )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    P199L
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177949-plasmid-map-sequence-id-348194

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Showing: 8601 - 8650 of 19190 results