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Plasmid#178213PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.C.S and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.C.HSV_mCherry-NLS
Plasmid#178210PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.C.HSV and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRvCAHS1-mEGFP-NLS
Plasmid#205013PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the cahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvSAHS1-mCherry-NLS
Plasmid#205009PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.DepositorInsertmCherry-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from R. varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc Dam_f_GFPoNLS
Plasmid#85820PurposeiDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc Dam_f_GFPoNLS
UseTagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A.PromoterAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-NLS-TurboID
Plasmid#163849PurposeIn vitro transcription of nls-TurboID. Parton lab clone JSTDepositorInsertTurboID
UseIn vitro transcription of rnaTagsnlsExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-mCherry-NLS
Plasmid#186346PurposeNucleus-targeted mCherry expression in mammalian cellsDepositorInsertmCherry-3xNLS
UseTagsExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN1-NLS-Q2m
Plasmid#129319PurposeQUEEN ATP sensor for 25degC, nucleusDepositorInsertQUEEN-2m
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-iRFP702-NLS
Plasmid#184454PurposeNucleus-targeted iRFP702 expression in mammalian cellsDepositorInsertiRFP702-3xNLS
UseTagsExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-Citrine-NLS
Plasmid#182583PurposeTransient expression of Citrine-NLS in plant cell (Nucleus)DepositorInsertNLS
UseTagsCitrineExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-HA-2xMCP-Ezh2
Plasmid#126590PurposeExpresses MCP (MS2 Coat Protein) fusion to Ezh2 in mammalian cells, lentiviral backboneDepositorInsert2XMCP-Ezh2 (Ezh2 Mouse, Synthetic)
UseLentiviralTagsNLS-HAExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS-foxc1a-2a-nlstdTomato
Plasmid#132970Purposefull-length of foxc1a in frame with viral 2a peptide and nuclear localized tdTomato in pCS expression vectorDepositorInsertsUseTagsNLS and mycExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-NLS
Plasmid#103819PurposeSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illuminationDepositorInsertPHR-mCherry-NLS (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry1-PCNA-19-SV40NLS-4
Plasmid#57192PurposeLocalization: Nucleus/Replication Origins, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertPCNA-19 SV40NLS (PCNA Human)
UseTags3x NLS and PA-mCherry1ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP-NLS
Plasmid#103820PurposeSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illuminationDepositorInsertPHR-EYFP-NLS (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-QUAS5x:GFPNLS-SV40pA
Plasmid#155115PurposeMiddle entry vector containing QUAS5x element upstream of GFP-NLS.DepositorInsertQUAS5x:GFPNLS
UseGateway middle entry vectorTagsExpressionMutationPromoterQUAS5x-E1bAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
RFP-eNOS-NLS
Plasmid#22496DepositorInserteNOS(G2A)-NLS
UseTagsRFPExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
MS2_adRNA-MCP_ADAR2 DD_NLS
Plasmid#124704PurposeExpresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD_NLSDepositorInsertMCP_ADAR2 DD_NLS
UseAAVTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
7xNLS-pMJ915v2-sfGFP
Plasmid#88922PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-NLS-tdMCP
Plasmid#86245PurposeMiddle entry vector for inserting promotors for tissue specific expression of NLS-tdMCPDepositorTypeEmpty backboneUseUnspecifiedTagsNLStdMCPExpressionMutationPromoterAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pST1374-Lb-Cpf1-NLS
Plasmid#107680PurposeExpresses LbCpf1 in mammalian cellsDepositorInsertLbCpf1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
RPL25NLS-GFP (101)
Plasmid#24038DepositorInsertRPL25 (RPL25 Budding Yeast)
UseTagsEGFPExpressionYeastMutationOnly bp 1-135 (aa 1-45) of RPL25PromoterAvailable sinceMarch 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
PS-CFP2-PCNA-19-SV40NLS-4
Plasmid#57245PurposeLocalization: Membrane Scaffold, Excitation: 400 / 490, Emission: 468 / 511DepositorInsertPCNA-19 SV40NLS (PCNA Human)
UseTagsPS-CFP2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV_hSyn_TALEBB(HD)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA
Plasmid#47450PurposeAAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.DepositorInsertTALE BB(N136_0.5HD_C63)-SID4X
UseAAV; TaleTags2A_phiLOV2.1ExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.HSV.VSVg_mCherry-NLS
Plasmid#178225PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.HSV.VSVg and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NLS-ZapCmR1.1
Plasmid#59011PurposeNuclear zinc-binding FRET biosensorDepositorInsertNLS-ZapCmR1.1 genetically encoded Zn(II) sensor
UseTagsSV40 NLSExpressionMammalianMutationSee commentPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
mPlum-PCNA-19-SV40NLS-4
Plasmid#55994PurposeLocalization: Nucleus, Excitation: 590, Emission: 649DepositorInsertPCNA-19 SV40NLS (PCNA Human)
UseTagsmPlumExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-3xNLS-OptoJNKi
Plasmid#89740PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, wild-type photosensor, inhibitor JIP11DepositorInsertNLS-OptoJNKi
UseFluorescent proteinTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-F-Gcn5-NLSm
Plasmid#63708Purposeretroviral expression of cytoplasm-localized mouse Gcn5 in mammalian cellsDepositorInsertGcn5 (Kat2a Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationmutated K425, K427 and K438 to R (arginine)PromoterAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB102-Citrine-NLS
Plasmid#79136PurposeBinary vectors for expression of Citrine fluorescent protein with a nuclear localization signal in plantsDepositorInsertCitrine-NLS
UseTagsNLS (Nuclear Localization Signal)ExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceAug. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS-nlstdTomato
Plasmid#67693PurposeTol1 expression plasmid with UAS-driven myc tagged nlsTdTomatoDepositorInsert6xMTnlsTomato
UseTags6xMyc-NLSExpressionMutationPromoterAvailable sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc-oDam_f_GFPoNLS
Plasmid#85818PurposeiDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc oDam_f_GFPoNLS
UseTagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A. Codon optimization compatible to work in M…PromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.HSV.Ollas_mCherry-NLS
Plasmid#178223PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.HSV.Ollas and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.HA.V5_mCherry-NLS
Plasmid#178221PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.HA.V5 and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.HA.Ollas_mCherry-NLS
Plasmid#178218PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.HA.Ollas and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.C.V5_mCherry-NLS
Plasmid#178215PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.C.V5 and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.C.Ollas_mCherry-NLS
Plasmid#178212PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.C.Ollas and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN1-NLS-Q7mu
Plasmid#129314PurposePositive control for ATP measurement, nucleusDepositorInsertQUEEN-7mu
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_TALEBB(NI)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA
Plasmid#47452PurposeAAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.DepositorInsertTALE BB(N136_0.5NI_C63)-SID4X
UseAAV; TaleTags2A_phiLOV2.1ExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_TALEBB(HD)-NLS-VP64_2A_GFP_WPRE_bGHpA
Plasmid#47446PurposeAAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.DepositorInsertTALE BB(N136_0.5HD_C63)-VP64
UseAAV; TaleTags2A_GFPExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only