We narrowed to 171,035 results for: Gene
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Plasmid#158059PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyExpressionBacterialPromoteracpPAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSC12
Plasmid#104786PurposepNB184-Cas9 entry cassetteDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD81-(TEV)-tTA
Plasmid#162598Purposehuman CD81-TEV cleavage site-tTADepositorInserthuman CD81 (CD81 Human)
TagsTEV protease cleavage site and tTAExpressionMammalianPromoterCMV promoterAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMZ-178
Plasmid#195426PurposeTransient, constitutive expression of synZiFTR: ZF3-p65DepositorInsertpUb - 3XFLAG - NLS - ZF3-5 (ZF3) - p65 - RbGpA
ExpressionMammalianAvailable SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMZ-185
Plasmid#195433PurposeTransient, constitutive expression of synZiFTR: ZF10-p65DepositorInsertpUb - 3XFLAG - NLS - ZF10-1 (ZF10) - p65 - RbGpA
ExpressionMammalianAvailable SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXGFPC-5 Pxyl-dcas9 (Sth3)
Plasmid#133317Purposeexpresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus thermophilus #3)
UseUnspecifiedPromoterxyloseAvailable SinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
SIK1_pCSdest
Plasmid#53936DepositorAvailable SinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAGM55285
Plasmid#153212PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST markerDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
P628_SpCas9-RecA
Plasmid#87263PurposeHuman codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiencyDepositorInsertRecA
UseCRISPRTagsFLAGExpressionMammalianMutationnoPromoterCBhAvailable SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pReporter_12
Plasmid#60572PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_12
ExpressionBacterialPromoterBBa_J23119Available SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-2
Plasmid#160702Purposegpdh promoter from Trichoderma virens bordered by BsaI restriction sitesDepositorInsertgpdh
UseSynthetic BiologyMutationIncorporation of BsaI restriction sitesAvailable SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSC10
Plasmid#104784PurposepAH595-gRNA 2xplex entry cassetteDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNeoXTR f0
Plasmid#69157Purposemouse targeting vectorDepositorInsertsDTA
NeoR
GFP
AmpR
UseMouse TargetingPromoterAmp promoter, PGK, and noneAvailable SinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVP2A-PIF6
Plasmid#73369PurposeExpresses Adeno-associated virus VP2 with PIF6 insert.DepositorAvailable SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMN-285
Plasmid#195472PurposeLentiviral Integratable Inducible CD19CAR using synZiFTR ZF10DepositorInsert8X ZF10 BS - ybTATA - CD19 CAR - mCherry
UseLentiviralExpressionMammalianAvailable SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
GLI-3 bs-2
Plasmid#16420DepositorInsertGLI3 (GLI3 Human)
ExpressionBacterialAvailable SinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKAd5f11pABR-EPG
Plasmid#122699PurposeIt can be used to construct a recombinant virus carrying other transgene or an adenovector with modified fiber.DepositorInsertGFP
UseAdenoviralAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mFzd1
Plasmid#42253DepositorInsertFrizzled 1 (Fzd1 Mouse)
ExpressionMammalianAvailable SinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
ibpAB-GFP
Plasmid#109390PurposeGFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertpibpAB-GFP
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
htpG1-GFP
Plasmid#109387PurposeGFP cassette under the control of the htpG1 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertphtpG1-GFP
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
PROM5_MpEF1a
Plasmid#136113PurposeStrong promoter from MpEF1a, that drives expression everywhere in Mp gemmae but stronger in the notchesDepositorInsertPROM5_MpEF1a Mapoly0024s0116
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEE393
Plasmid#149283PurposeT-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbPDS3DepositorInsertp35S:TRV2_NbPDS3sgRNA_TrunFT:tNos
ExpressionPlantPromoter35SAvailable SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
IZ501: pMVP/Lenti-DEST
Plasmid#121848PurposepMVP destination vector, empty Lentivirus vector backboneDepositorTypeEmpty backboneUseLentiviral; Pmvp gateway destination vectorAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH3
Plasmid#52528Purposeserves as PCR-template to generate HR donors for C-terminal GFP-tagDepositorInserteGFP and Blasticigin resistance cassette
UseBlunt cloning kitAvailable SinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
3521 pET15b Bid p15
Plasmid#8782DepositorAvailable SinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pXEE401BG
Plasmid#91717PurposeCRISPR/Cas9-mediated genome editing in Arabidopsis, Bar and glyphosate-resistanceDepositorTypeEmpty backboneExpressionPlantAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pQdCas12a.sggfp(C)
Plasmid#236042PurposeThe plasmid pQdCas12a.sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene.DepositorInsertquorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
UseCRISPRPromoterquorum sensing promoterAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-I48V_AnaCNJKFGW_Bba-B0015
Plasmid#202023PurposeExpresses Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant) in E. coliDepositorInsertAnabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant)
ExpressionBacterialMutationGvpA T6A-I48VAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-memRFP-3xControlgRNA
Plasmid#224567PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.DepositorInsertmRFP1
UseCRISPRTagsMembrane localization signalMutationCodon optimizedAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLC-238
Plasmid#73192PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (chpB) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorAvailable SinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GBP6-10gly-VPminx4
Plasmid#50796PurposeExpression of a GFP-dependent transcription factor component in mammalian cellsDepositorInsertGBP6-VPminx4 activation domain fusion protein
ExpressionMammalianPromoterCAGAvailable SinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
P823_eSpCas9(1.1)-RecA
Plasmid#87264PurposeHuman codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiencyDepositorInsertRecA
TagsFLAGExpressionMammalianPromoterCBhAvailable SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mFzd9
Plasmid#42261DepositorInsertFrizzled 9 (Fzd9 Mouse)
ExpressionMammalianAvailable SinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-3xGFP11-mem-3xControlgRNA
Plasmid#224583PurposeUbiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.DepositorInsert3xGFP11
UseCRISPRTagsMembrane Localization SignalMutationCodon optimizedAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_hsNotch3 LNR
Plasmid#188338PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch3-ECD, a Notch3-TMD, a Notch3-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_Notch3-ECD_Notch3-TMD_Notch3-JMD_Gal4VP64
UseLentiviralAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_FAP site with scFv
Plasmid#188351PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a FAPsite, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_FAPsite_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNL(CMV)Fluc/CMV/WPREdU3
Plasmid#136881PurposeReporter vector expression firefly luciferaseDepositorInsertFirefly luciferase
UseLentiviralAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-OCT4
Plasmid#69537PurposeEpisomal plasmid encoding 5 gRNAS targeting human OCT4 promoterDepositorInsert5 concatenated gRNA transcriptional cassettes targeting OCT4
UseCRISPRAvailable SinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-iRFP670-Tdtomato-GFP
Plasmid#111817PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsiRFP670
Tdtomato
GFP
UseCloning intermediateAvailable SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-rTetRDBD-GBP1
Plasmid#50791PurposeExpression of a GFP-dependent transcription factor component in mammalian cellsDepositorInsertrTetR DNA binding domain-GBP1 fusion protein
ExpressionMammalianPromoterCAGAvailable SinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
EC71100
Plasmid#154068PurposeLevel 0 Golden Gate vector, pHvHSP17DepositorInsertpHvHSP17
UseStandard cloning vector used by geneart for synth…MutationAll BsaI, Esp3I and BpiI restriction sites were r…Available SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HemmarR2
Plasmid#112813Purposegateway cloning destination vector for expressing enhancer driven CD4-tdTomatoDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
PGK+ plasmid with onboard mCherry cassette
Plasmid#169745PurposePGK+ plasmid that also encodes separate mCherry cassette. mCherry is used to monitor plasmid dosage.DepositorInserteGFP
UseSynthetic BiologyExpressionMammalianPromoterPGK-tetO2Available SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRII-1.1-1.10a
Plasmid#69589PurposeOT-II TCR a chain cDNA in pCRIIDepositorInsertTCR alpha clone 1.1 (Tcra Mouse)
ExpressionBacterialAvailable SinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR-P2A-BlastR
Plasmid#98147PurposeExpresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica.DepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsP2A-BlastR fusion with Cas9-NluxPromoterRibi promoterAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-AMOT p130 Y287A
Plasmid#32824DepositorAvailable SinceJan. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pCAG/Luci
Plasmid#139981PurposeExpresses luciferase under the promoter of CAG. Transgene flanked by AAV2 ITRDepositorInsertLuciferase
UseAAVPromoterCAGAvailable SinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKB01-PssbB-mKate2
Plasmid#69105PurposeExpresses mKate2 (codon adapted for Streptococcus pneumoniae); under control of CSP inducible promoter PssbB. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.DepositorInsertmKate2
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only