165,187 results

1. AAVS1_gRNA

   Plasmid

   #196139

   Purpose

   gRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFP

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   AAVS1 gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-PIK3CA-H1047R

   Plasmid

   #116500

   Purpose

   Lentiviral expression of PIK3CA H1047R

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Lentiviral

   Mutation

   H1047R

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-C-mScarlet-Puro

   Plasmid

   #207722

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. qTAG-N-Puro-mStayGold-ACTB

   Plasmid

   #227320

   Purpose

   Donor template for Puro-2A-mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Puro-2A-mStayGold Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-EML4-ALK variant 1

   Plasmid

   #183828

   Purpose

   Express EML4-ALK fusion variant 1 (E13;A20) in mammalian cells

   Depositor

   Aaron Hata

   Article

   Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

   Inserts

   ALK Receptor Tyrosine Kinase (ALK Human)
   EMAP Like 4 (EML4 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of EML4 exon 1-13 and ALK exon…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. NW9

   Plasmid

   #133442

   Purpose

   Covalently circularized nanodisc NW9 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW9
   

   Expression

   Bacterial

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1(+) miniTurboID-6xGGSG linker-FKBPF36V-2xHA

   Plasmid

   #200641

   Purpose

   Expresses miniTurboID-FKBPF36V-2xHA in mammalian cells.

   Depositor

   Justin English, Fleur Ferguson

   Article

   Tao et al Nat Commun. 2023 Dec 4;14(1):8016. doi:

   Insert

   miniTurboID-6xGGSG linker-FKBPF36V-2xHA
   

   Tags

   HA-tag

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. gag-PE

   Plasmid

   #211371

   Purpose

   Packaging plasmid for v3 PE-eVLP production

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   MMLVgag-PE
   

   Expression

   Mammalian

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV CMV FST-FLAG

   Plasmid

   #175167

   Purpose

   Lentiviral expression of human FST-FLAG

   Depositor

   Larry Gerace

   Insert

   FST (FST Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCH45 (multiAsCas12a-KRAB lenti)

    Plasmid

    #217330

    Purpose

    Lentiviral expression of multiAsCas12a-KRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    multiAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

    Mutation

    R1226A/E174R/S542R/K548R

    Promoter

    UCOE-SFFV

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAJE-Ma pylRS nitroY/haloY-F5

    Plasmid

    #225684

    Purpose

    Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.

    Depositor

    Ryan Mehl

    Inserts

    M. alvus PylRS 3-NY/HaloY F5
    M. alvus Pyl-tRNA(6)
    

    Use

    Methanomethylophilus alvus

    Mutation

    CTG125CTT, N166A, V168S, A223C, W239R

    Promoter

    GlnS and Lpp

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223_FYN_WT

    Plasmid

    #82211

    Purpose

    Gateway Donor vector containing FYN , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    FYN (FYN Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-HsBIN1_tv8

    Plasmid

    #176012

    Purpose

    Mammalian expression of Human EGFP-Bin1 (transcriptional variant 8, muscle specific). Parton lab clone HBH

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiGuideFE-Puro

    Plasmid

    #170069

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoter

    Depositor

    Neville Sanjana

    Article

    Morris et al Science. 2023 May 19;380(6646):eadh7699.

    Insert

    Cas9 guide RNA scaffold with the F+E scaffold modification
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS40P

    Plasmid

    #35481

    Depositor

    Steven Haase

    Article

    Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

    Type

    Empty backbone

    Use

    Yeast integrating vector

    Available Since

    Nov. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAB167

    Plasmid

    #101672

    Purpose

    Opto-T7RNAP*(563), equal expr.

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    Opto-T7RNAP*(563), equal expr.
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

    Plasmid

    #179116

    Purpose

    Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    Cargo EGFP with AttP Bxb1 site for in-frame fusion
    

    Use

    CRISPR

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP-Vimentin-7

    Plasmid

    #56439

    Purpose

    Localization: Intermediate Filaments, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Insert

    Vimentin (VIM Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pHJ12: CadC-VHH-Caffeine (No linker)

    Plasmid

    #108244

    Purpose

    Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression

    Depositor

    Jerome Bonnet

    Article

    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

    Insert

    Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
    

    Use

    Synthetic Biology

    Promoter

    pLacO1

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-GFP-CD63

    Plasmid

    #194989

    Purpose

    overexpress CD63

    Depositor

    Angelique Bordey

    Insert

    GFP-CD63
    

    Expression

    Bacterial

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCMV7.1_3xFLAG-LATS2

    Plasmid

    #172987

    Purpose

    constitutive expression of FLAG-tagged LATS2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-LATS2 (LATS2 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLentiCMV_HyPer7RgDAAO

    Plasmid

    #217653

    Purpose

    Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane

    Depositor

    Seth Parker

    Article

    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

    Insert

    HyPer7 D amino acid oxidase
    

    Use

    Lentiviral

    Tags

    Nuclear export signal

    Expression

    Mammalian

    Mutation

    Fused DAAO to the C-terminus of HyPer7 using a Gl…

    Promoter

    CMV

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pYX122-mtGFP

    Plasmid

    #45048

    Depositor

    Benedikt Westermann

    Article

    Westermann et al Yeast. 2000 Nov;16(15):1421-7.

    Insert

    mtGFP
    

    Expression

    Yeast

    Promoter

    TPI

    Available Since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

24. pLVX-Tight-Puro_MYRF

    Plasmid

    #64843

    Purpose

    A lentiviral vector for the expression of mouse MYRF under doxycyline control.

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    MYRF (Myrf Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPICZ-pre-pro-alphaf(I)-E2-Crimson

    Plasmid

    #117660

    Purpose

    Test intracellular localisation of E2-Crimson and study the secretion efficiency

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-pro-alphaf(I)-E2-Crimson
    

    Expression

    Yeast

    Mutation

    This plasmid encodes the fluorescent protein E2-C…

    Promoter

    pAOX1

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin

    Plasmid

    #134858

    Purpose

    Expresses EGFP-tagged early endosome marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Ras-related protein 5A
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEF-Bos TRIF Flag

    Plasmid

    #41550

    Depositor

    Kate Fitzgerald, Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    Trif (TICAM1 Human)
    

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

28. pLKO.1-sh-mSOX9-5

    Plasmid

    #40646

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-mSOX9-5 (Sox9 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

29. pET-45b(+)-Tn5

    Plasmid

    #112112

    Purpose

    Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

    Depositor

    Frank Pugh

    Article

    Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

    Insert

    Tn5
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E54K E110K P242A L372P

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. negMaLionG

    Plasmid

    #113907

    Purpose

    negative control for Green ATP sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

    Insert

    negMaLionG
    

    Expression

    Mammalian

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TFORF3476

    Plasmid

    #144952

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SNAI1 (SNAI1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMA7CR_2.0

    Plasmid

    #73950

    Purpose

    pMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recX

    Depositor

    Alex Nielsen

    Article

    Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

    Inserts

    cas9
    lambda beta
    dam
    recX
    

    Expression

    Bacterial

    Promoter

    pAra and pTet

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-dAPEX2

    Plasmid

    #117173

    Purpose

    Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    dAPEX2
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pVS1

    Plasmid

    #33143

    Depositor

    Michael Rosbash

    Article

    Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

    Insert

    LacO (256 copies)
    

    Expression

    Yeast

    Available Since

    Dec. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

35. pIntegrase_8

    Plasmid

    #60579

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_8
    

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. p(HA)HIF1alpha (P402A,P564A)

    Plasmid

    #52636

    Purpose

    mammalian expression of HA tagged HIF1alpha (full length) with P402A,P564A mutation

    Depositor

    Eric Huang

    Article

    Kageyama et al FASEB J. 2004 Jun;18(9):1028-30. Epub 20

    Insert

    HIF1alpha P402A, P564A (HIF1A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    P402A, P564A

    Promoter

    CMV

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDONR-A-HYG

    Plasmid

    #29635

    Depositor

    David_L Andrews, Scott Gold

    Article

    Garcia-Pedrajas et al Fungal Genet Biol. 2008 Apr . 45(4):379-

    Type

    Empty backbone

    Use

    Gateway donor

    Available Since

    Oct. 5, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pCINeo-myc-Itch/2859

    Plasmid

    #11427

    Depositor

    Allan Weissman

    Article

    Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

    Insert

    Itch (Itch Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    n/a

    Available Since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. HA-HIF2alpha-P405A/P531A-pBabe-puro

    Plasmid

    #19006

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: P405A and P531A

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pWZL Neo Myr Flag ADRBK1

    Plasmid

    #20418

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    ADRBK1 (GRK2 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only