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Addgene

We narrowed to 26,673 results for: Cat

Showing: 951 - 1000 of 26673 results
  1. IBMc460

    Plasmid
    #161940
    Purpose
    Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter
    Depositor
    Insert
    pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PPhlF
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pBP-ORF

    Plasmid
    #72949
    Purpose
    Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site
    Depositor
    Insert
    Level 0 - ORFs
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
  3. IBMc180

    Plasmid
    #161942
    Purpose
    Carries Golden Gate substitution insert for acVHH-assisted bisection mapping
    Depositor
    Insert
    pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pTU2-D-lacZ

    Plasmid
    #72953
    Purpose
    Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE
    Depositor
    Insert
    Level 2 - D site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
  5. pTU2-D-RFP

    Plasmid
    #72957
    Purpose
    Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE
    Depositor
    Insert
    Level 2 - D site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
  6. pRedCmOcr

    Plasmid
    #176226
    Purpose
    A helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.
    Depositor
    Insert
    Lambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaB and PL
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pMS_366

    Plasmid
    #182131
    Purpose
    expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation
    Depositor
    Inserts
    retron RNA
    ec.86 RT
    CspRecT
    Use
    Tags
    Expression
    Mutation
    incorporated rifampicin-resistance-conferring don…
    Promoter
    Available since
    Sept. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pBM019

    Plasmid
    #128179
    Purpose
    Construct for generating stable Cas9-expressing Toxoplasma gondii
    Depositor
    Inserts
    sgRNA#1
    SpCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…
    Available since
    July 10, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. pDEINO1

    Plasmid
    #179472
    Purpose
    Multi-host shuttle plasmid for D. radiodurans, E. coli, S. cerevisiae, S. meliloti, and P. tricornutum; contains an oriT for transfer via conjugation
    Depositor
    Inserts
    ori
    cat
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. pMS_375

    Plasmid
    #182132
    Purpose
    expresses retron RNA, ec.86 Reverse Transcriptase, CspRecT SSAP, and mutL* to create a specific rifampicin resistance mutation while inhibiting MMR
    Depositor
    Inserts
    retron RNA
    ec.86 RT
    CspRecT
    Use
    Tags
    Expression
    Mutation
    incorporated rifampicin-resistance-conferring don…
    Promoter
    Available since
    Sept. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. pCMV-MA-VN

    Plasmid
    #123283
    Purpose
    Mammalian expression plasmid for matrix domain of HIV-1 Gag fused to the N-terminal half of split fluorescent Venus (VN)
    Depositor
    Insert
    Matrix
    Use
    Tags
    VN: N-terminus of split Venus (a.a. V1–A154) I152…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
  12. pUDP123

    Plasmid
    #107269
    Purpose
    pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)
    Depositor
    Insert
    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. pAJC1

    Plasmid
    #240164
    Purpose
    Production of a thermostable enzyme, Pyrobaculum calidifontis manganese-dependent catalase, to be used in an educational laboratory exercise.
    Depositor
    Inserts
    katPc
    Pc- kat
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
  14. pUCsRNAP

    Plasmid
    #182746
    Purpose
    Swapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmids
    Depositor
    Insert
    small RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
  15. pCHC036 (∆pHG1)

    Plasmid
    #226206
    Purpose
    Conjugation knockout plasmid (pK18msB) to delete the C. necator megaplasmid replication region containing helD, repA, repB, and parAB in order to promote loss of the entire pHG1 megaplasmid.
    Insert
    Homology arms for the pHG1 megaplasmid replication region
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    ∆helD ∆repA ∆repB ∆parAB
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  16. pAAV Syn Barcode13

    Plasmid
    #226187
    Purpose
    Expression mapping
    Depositor
    Insert
    Syn Barcode13
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
  17. pAAV Syn Barcode19

    Plasmid
    #226196
    Purpose
    Expression mapping
    Depositor
    Insert
    Syn Barcode19
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
  18. A3Ai E72A-Cas9n-UGI-NLS

    Plasmid
    #109430
    Purpose
    Expresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Insert
    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (APOBEC3A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
  19. A3Bi-ctd E255A -Cas9n-UGI-NLS

    Plasmid
    #109429
    Purpose
    Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Insert
    Apolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (APOBEC3B Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
  20. pSJX022

    Plasmid
    #232325
    Purpose
    Genome editing in Caulobacter crescentus
    Depositor
    Inserts
    sgRNA
    SpCas9M
    Use
    CRISPR
    Tags
    H-arms and cat
    Expression
    Bacterial
    Mutation
    Promoter
    PJ23119 and Pvan
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  21. pSJX024

    Plasmid
    #232327
    Purpose
    Genome editing in Caulobacter crescentus
    Depositor
    Inserts
    sgRNA
    SpCas9M
    Use
    CRISPR
    Tags
    H-arms and cat
    Expression
    Bacterial
    Mutation
    Promoter
    PJ23119 and Pxyl
    Available since
    June 16, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 951 - 1000 of 26673 results