161,186 results

1. pET21a-SerRS-His

   Plasmid

   #124118

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Seryl-tRNA ligase (serS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pQE60-EFG-His

   Plasmid

   #124126

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Elongation factor G (fusA Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NTHL1-mCherry

   Plasmid

   #215145

   Purpose

   Expresses NTHL1-mCherry in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   NTHL1 (NTHL1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJBM4

   Plasmid

   #225291

   Purpose

   E. coli-Campylobacter shuttle vector; TetR

   Depositor

   Robert Moore

   Article

   McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

   Insert

   Tet(O) and its putative promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mVenus-proα1(I)

   Plasmid

   #119845

   Purpose

   Expresses mouse Type I procollagen α1 chain (Col1a1) with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Article

   Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

   Insert

   Type I procollagen α1 chain (Col1a1 Mouse)
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Col1a1 exons 2-3 replaced by fluorescent tag

   Promoter

   CMV

   Available since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJC005.em

   Plasmid

   #182738

   Purpose

   CRISPR-Cas12a genome editing of E. faecium

   Depositor

   James Collins

   Article

   Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. COMTD1

   Plasmid

   #25484

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   COMTD1 (COMTD1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 23, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

8. Lenti_gRNA-Puro

   Plasmid

   #84752

   Purpose

   Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABE YAP1

   Plasmid

   #15682

   Depositor

   Joan Brugge

   Article

   Overholtzer et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

   Insert

   YAP (YAP1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mCherry-DFCP1

    Plasmid

    #86746

    Purpose

    N-terminal mCherry-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

    Insert

    human DFCP1 (ZFYVE1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pScaf

    Plasmid

    #111401

    Purpose

    Phagemid for producing DNA origami scaffolds, derived from pUC18 and M13mp18

    Depositor

    Shawn Douglas

    Article

    Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mCerulean3-ER-5

    Plasmid

    #55418

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    ER (CALR Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    ER Signal Peptide

    Promoter

    CMV

    Available since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pMOS023E: Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)

    Plasmid

    #163077

    Purpose

    Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    Peredox
    

    Use

    Gateway entry vector

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CIP4-pmCherryC1

    Plasmid

    #27685

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    CIP4 (Trip10 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1-CHRNA7-mGFP

    Plasmid

    #62629

    Purpose

    mammalian expression of human Alpha7 (CHRNA7) with mEGFP fused into M3-M4 loop

    Depositor

    Henry Lester

    Article

    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

    Insert

    hAlpha7 (CHRNA7 Human)
    

    Use

    Tags

    mEGFP inserted in M3-M4 loop

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-TRE-tdTomato-WPRE

    Plasmid

    #104112

    Purpose

    AAV-mediated expression of tdTomato under the TRE promoter.

    Depositor

    Takeshi Imai

    Article

    Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV6-AN-HA_WT POLK

    Plasmid

    #201584

    Purpose

    Expresses human DNA polymerase kappa with an N-terminal HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    Polymerase kappa (POLK Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SETD2-GFP

    Plasmid

    #80653

    Purpose

    mammalian expression of SETD2-GFP

    Depositor

    Sérgio De Almeida

    Article

    Carvalho et al Elife. 2014 May 6;3:e02482. doi: 10.7554

    Insert

    SETD2 (SETD2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. OMMGFP 1-10

    Plasmid

    #164119

    Purpose

    Expresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMM

    Depositor

    Tito Calì, Marisa Brini

    Article

    Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

    Insert

    OMMGFP 1-10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-CAG-FLPX-rc [Jaws-KGC-tdTomato-ER2]

    Plasmid

    #195488

    Purpose

    AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in FLPX/reversed (Frt-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-tdTomato-ER2
    

    Use

    AAV

    Tags

    ER2, KGC, and tdTomato

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    CAG

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. hyABE

    Plasmid

    #196096

    Purpose

    Expresses hyABE

    Depositor

    Xiahui Zhang

    Article

    Xue et al Nat Commun. 2023 Mar 3;14(1):1224. doi:

    Insert

    hyABE
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pT/CAGGS-mVenus-P2A-NRASG12V

    Plasmid

    #236072

    Purpose

    Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and mutant NRAS (NRASG12V).

    Depositor

    Masashi Narita

    Article

    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

    Inserts

    mVenus
    NRAS G12V (NRAS Human)
    

    Use

    Sleeping beauty transposon plasmid

    Tags

    Expression

    Mutation

    G12V

    Promoter

    CAGGS

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-cytoFLARE1.0/1.1-TF

    Plasmid

    #234516

    Purpose

    Transcriptional reporter for detecting calcium transients in neuronal cultures (transcription factor)

    Depositor

    Wenjing Wang

    Article

    Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

    Insert

    ERT2-MK2-hLOV-TEVcs-FLAG-tTA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. A24M04 mIgM heavy chain-pFEEKW

    Plasmid

    #234809

    Purpose

    Express membrane form of IgM heavy chain containing the VH region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1

    Depositor

    Susan Pierce

    Article

    Torgbor et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

    Insert

    A24M04 membrane IgM heavy chain (IGHM Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EEK promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TFORF1727

    Plasmid

    #142837

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF692 (ZNF692 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLL5.0-MRLC-WT-GFP

    Plasmid

    #187258

    Purpose

    Lentiviral expression of myosin regulatory light chain-WT

    Depositor

    Alpha Yap

    Article

    Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

    Insert

    MRLC1 (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    5' LTR

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. DTX2

    Plasmid

    #228447

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Article

    Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

    Insert

    DTX2 (DTX2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. gRNA-10Xcapture-PuroR

    Plasmid

    #211496

    Purpose

    Plasmid for the expression of SAM-compatible gRNA for CRISPRa with capturer sequence for 10X Genomics sequencing

    Depositor

    Lesley Forrester, Antonella Fidanza

    Article

    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

    Inserts

    U6-gRNA
    Ef1a-PuroR
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and U6

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGL4_10xUAS-CMVmin-BC0396-luc2

    Plasmid

    #227115

    Purpose

    Barcoded assay, UAS sensor for split TEV assays; barcode BC0396

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    10x clustered UAS element linked to CMV minimal promoter driving barcode 0396 and a luciferase reporter gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPBbsr2-AREG-ScNeo

    Plasmid

    #209910

    Purpose

    To monitor the status of Amphiregulin, the plasmid encodes a recombinant AREG fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    AREG-ScNeo (AREG Human)
    

    Use

    Tags

    mNeonGreen and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. qTAG-C-Solo-mStayGold-CANX

    Plasmid

    #227280

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the CANX locus. For ER visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CANX Homology Arms flanking a mStayGold Tag (CANX Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGLU_66

    Plasmid

    #225769

    Purpose

    This is the native promoter from the catP chloramphenicol resistance cassette.

    Depositor

    Nili Ostrov

    Insert

    PcatP_[abr]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLenti-nSpCas9-evoCDA1

    Plasmid

    #209042

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    evoCDA1-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pROSA26-SAT

    Plasmid

    #208339

    Purpose

    ROSA26 targeting vector for the SAT sensor for both ERK1/2 and AKT signaling

    Depositor

    Philippe Soriano

    Article

    Dinsmore et al Dev Biol. 2023 Sep 1:S0012-1606(23)00146

    Insert

    SAT sensor
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCA_e-1

    Plasmid

    #202096

    Purpose

    Receiver cloning vector for even-1 level constructs. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pL0R_lacZ

    Plasmid

    #202095

    Purpose

    Receiver cloning vector for making level 0 parts. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pCA_o-1

    Plasmid

    #202091

    Purpose

    Receiver cloning vector for odd-1 level constructs. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. TFORF3072

    Plasmid

    #144548

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NELFE (NELFE Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAG-FIP200 (1-640)-TSF

    Plasmid

    #203545

    Purpose

    FIP200 NTD-TwinStrep-Flag mammalian expression

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Tags

    TwinStrep Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TFORF1835

    Plasmid

    #143323

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EZH1 (EZH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLX317-VRK2

    Plasmid

    #199641

    Purpose

    Expresses VRK2

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK2 (VRK2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. TFORF3342

    Plasmid

    #144818

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TP53BP2 (TP53BP2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. TFORF2103

    Plasmid

    #141924

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZBTB32 (ZBTB32 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCDH_CMV_dimer_mKate2

    Plasmid

    #167333

    Purpose

    Expresses mKate2 fused to TFAM no-dimerization mutant in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Lentiviral

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Changed K95A, Y99F, E106A, E112A and R116A

    Promoter

    CMV

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. pCDH_CMV_HMGA_mKate2

    Plasmid

    #167334

    Purpose

    Expresses mKate2 fused to TFAM HMGA mutant in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Lentiviral

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Truncation mutant containing MTS (1-42) and HMGA …

    Promoter

    CMV

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCDH_CMV_dC_mKate2

    Plasmid

    #167336

    Purpose

    Expresses mKatet2 fused to TFAM dC-tail mutant in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Lentiviral

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Truncation mutant containing MTS (1-42) as well a…

    Promoter

    CMV

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. GST-RBD

    Plasmid

    #15247

    Depositor

    Martin Schwartz

    Article

    Ren et al EMBO J. 1999 Feb 1. 18(3):578-85.

    Insert

    Rhotekin (Rtkn Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 7-89 (Rho binding domain)

    Promoter

    Available since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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48. pKRG3-mU6-PUb-3xFLAG-hSpCas9

    Plasmid

    #162161

    Purpose

    Expresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. pcDNA3.1 SIRT7_H187Y

    Plasmid

    #53151

    Purpose

    expression vector for catalytically inactive human Sirtuin7

    Depositor

    Katrin Chua

    Article

    Shin et al Cell Rep. 2013 Nov 14;5(3):654-65. doi:

    Insert

    Sirt7 (SIRT7 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H187Y, catalytic inactive

    Promoter

    CMV

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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50. pBS513 EF1alpha-cre

    Plasmid

    #11918

    Purpose

    Expresses Cre under the control of the elongation factor-1 alpha (EF1alpha) promoter; includes upstream Kozak modification to increase expression.

    Depositor

    Brian Sauer

    Article

    Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

    Insert

    cre (cre bacteriophage P1)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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