19,210 results
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pBP Omomer
Plasmid#113169PurposeRetroviral vector for expressing Omomer (tamoxifen inducible Omomyc), by DNA transfection or retroviral infection.DepositorInsertOmomer: a fusion contruct of Omomyc and MER (a mutant estrogen receptor, reponsive to tamoxifen) (MYC Human)
UseRetroviralTagsExpressionMammalianMutationE57T, E64I, R70Q, R71NPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.2
Plasmid#111054PurposeExpresses dLight1.2 in mammalian cellsDepositorInsertdLight1.2
UseTagsFlag tagExpressionMammalianMutationPromoterCAGAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMPRA1
Plasmid#49349PurposeBasic backbone for transient transfection of Massively Parallel Reporter Assay (MPRA) librariesDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-aBEST
Plasmid#131464PurposeExpression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetesDepositorInsertsStreptomyces codon optimized spCas9n (D10A)
streptomyces codon optimized tadA
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromotertipAAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cry2FL-VP64
Plasmid#60554PurposeExpresses Cry2FL-VP64 in mammalian cellsDepositorInsertCry2FL-VP64
UseTagsFLAG, HA tag, and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(C128S)-EYFP-WPRE
Plasmid#20294PurposeLentiviral expression of ChR2 (C128S) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsEYFPExpressionMammalianMutationchanged Cysteine 128 to SerinePromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAV-P(Per2)-DIO-intron2-dLUC
Plasmid#110059PurposePer2 transcription luciferase reporterDepositorInsertPer2 promoter and luciferase (Per2 Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterPer2Available sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Myc-IRES-RFP
Plasmid#35395DepositorInsertsUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSin-EF2-LIN28-Pur
Plasmid#16580DepositorInsertHomo sapiens lin-28 homolog (C. elegans) (LIN28A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dendra2-Actin-C-18
Plasmid#57701PurposeLocalization: Actin, Excitation: 490 / 553, Emission: 507 / 573DepositorInsertActin (ACTB Human)
UseTagsDendra2ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFPN1-gamma-tubulin
Plasmid#87956PurposeExpresses human gamma-tubulin with a GFP-tag on the C-terminal of the proteinDepositorInsertgamma-tubulin 1 (TUBG1 Human)
UseTagsGFPExpressionMammalianMutationThe protein is a sh-gamma-tubulin resistant gene …PromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2-Gateway
Plasmid#37846DepositorTypeEmpty backboneUseZebrafish enhancer assay vectorTagsExpressionMutationPromoterE1bAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
rAAV2/cMG.QRP
Plasmid#184543PurposeAAV packaging plasmid, expressing Rep2 and AAV-cMG.QRP capsidDepositorInsertAAV-cMG.QRP capsid
UseAAV; Helper vector for packagingTagsExpressionMutationchanges on AAV9 capsid: K449R; insertion of QRPPR…PromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXPR_206
Plasmid#96920Purposefor SaCas9 knockout, lentiviral expression of S. aureus Cas9 (SauCas9) and gRNA scaffoldDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-MEK-DD
Plasmid#15268DepositorInsertMap2k1 (Mapk1 Mouse)
UseRetroviralTagsExpressionMammalianMutationS218D S222DPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
TDP43 NOTAG1
Plasmid#28206DepositorInsertTar DNA binding protein (TARDBP Human)
UseTagsExpressionMammalianMutationFull lengthPromoterAvailable sinceJuly 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CA-E542K
Plasmid#116479PurposeLentiviral expression of PIK3CA E542KDepositorInsertPIK3CA (PIK3CA Human)
UseLentiviralTagsExpressionMutationE542KPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-ABI-dCas9-P2A-mcherry
Plasmid#121513Purposeexpression of AB1-fused-dcas9 plasmidsDepositorInsertdCas9
UseLentiviralTagsAB1 and P2A-mcherryExpressionMammalianMutationPromoterPGKAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR iSH-YFP-PIF
Plasmid#50841PurposeOptogenetic PI3K activation system; cytoplasmic componentDepositorInsertsp85 iSH2
PIF6 1-100
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1mychisA XDH
Plasmid#10958DepositorInsertxanthine dehydrogenase (XDH Human)
UseTagshis and mycExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJY-RpABE
Plasmid#112872Purposebinary vector for ABE expression in ArabidopsisDepositorInsertsRPS5A promoter
ABE7.10
U6-sgRNA cassette
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGF1-4eCOL2A1
Plasmid#97210PurposeLentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoterDepositorInsert4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterCOL2A1 regulatory elementsAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-Sox9
Plasmid#36979DepositorInsertSox9 (Sox9 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-AK1-His
Plasmid#118977PurposeBacterial expression plasmid for Adenylate kinase isoenzyme 1DepositorInsertadenylate kinase isoenzyme 1 AK1 (AK1 Chicken)
UseTags6x-HisExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Neo-Bam E2F3a
Plasmid#37970DepositorInsertE2F3a (E2F3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-shRab27a
Plasmid#120930Purposeconditionally knockdown Rab27a in mouse cell linesDepositorInsertshRab27a (Rab27a Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-ChR2(H134R)-eYFP
Plasmid#127090PurposeCre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activationDepositorHas ServiceAAV PHP.eBInsertChannelrhodopsin-2
UseAAVTagseYFP (C terminal on insert)ExpressionMammalianMutationHumanized ChR2 gene with histidine 134 changed to…PromoterCAGAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDULE-ABK
Plasmid#49086PurposeA pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experimentsDepositorInsertstRNA synthetase
tRNA for a photocrosslinking amino acid
UseTagsExpressionBacterialMutationL274M, C313A, Y349FPromoterGlnRS and lppAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-DEST-MTHFD2
Plasmid#31213DepositorInsertMTHFD2 (MTHFD2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SpCas9-GFP-U6-gRNA
Plasmid#79144PurposeAll-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSCDepositorInsertSpCas9
UseCRISPRTags2A-GFPExpressionMammalianMutationPromoterCAGAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMB-SaCas9
Plasmid#105998PurposeSaCas9 expression vectorDepositorInsertCas9 nuclease
UseCRISPR and LentiviralTagsnoneExpressionMammalianMutationnonePromotershort EF1alphaAvailable sinceJuly 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSADdeltaG-F3
Plasmid#32634DepositorInsertB19N, B19P, B19M and B19L
UseTagsExpressionMammalianMutationIn frame insertion of Ser after aa10 in M (matrix…PromoterCMVAvailable sinceOct. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Rac1-wt
Plasmid#12980DepositorInsertRac1 (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-ChR2-Venus
Plasmid#15753PurposeExpresses ChR2-Venus for neuronal excitation.DepositorInsertChannelrhodopsin2-Venus
UseTagsVenusExpressionMammalianMutationPromoterCAGAvailable sinceOct. 31, 2007AvailabilityAcademic Institutions and Nonprofits only -
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single polypeptide FPX biosensor for calcium ion
Plasmid#60887PurposeFPX biosensorDepositorInsertsingle polypeptide FPX biosensor for calcium ion
UseTagsHindIII siteExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLXV-EF1alpha-2xStrep-SARS-CoV-2-nsp14-IRES-Puro
Plasmid#141380PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp14 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLNCX2-mCherry-VPS4A
Plasmid#115334Purposeexpresses mCherry-VPS4A in mammalian cellsDepositorInsertVPS4A (VPS4A Human)
UseRetroviralTagsmCherryExpressionMammalianMutationsilent mutation in position 4050 C to TPromoterCMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP DNAJB1
Plasmid#19495DepositorInsertDNAJB1 (DNAJB1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
RANLS
Plasmid#50843PurposeExpresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.DepositorInsertddRFP A
UseTagstriplicated NLS sequence DPKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS PKC theta
Plasmid#8426DepositorInsertMouse Protein Kinase C theta (Prkcq Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 22, 2005AvailabilityAcademic Institutions and Nonprofits only -
CASR-Tango
Plasmid#66235PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertCASR (CASR Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
ankG-mCherry
Plasmid#42566DepositorInsertankyrin G (Ank3 Rat)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NMHC II-C
Plasmid#10843DepositorInsertNMHC II-C (Myh14 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDiGc
Plasmid#59322PurposeInducible expression of dsRed and constitutive expression of GFP in bacteriaDepositorInsertsEGFP
dsRed
UseTagsExpressionBacterialMutationPromoterPbad and rpsMAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
p4xACO-Luc
Plasmid#16533DepositorInsert4xACO
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
FRP2370
Plasmid#127576PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Leu2MX selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorTagsExpressionYeastMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-MCP-3XBFPnls
Plasmid#75384PurposeMCP-3XBFPnlsDepositorInsertMCP
UseLentiviralTags3XBFPExpressionMammalianMutationPromoterEFSAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
TALED_Left-ND1-AD-E1347A
Plasmid#183894PurposeExpresses TALED_Left-ND1-AD-E1347A in mammalian cellsDepositorInsertTALED for ND1 mutation
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only
