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pBK1290-AAV-dSaCas9
Plasmid#223139PurposeAAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA ScaffoldDepositorTypeEmpty backboneUseAAV and CRISPRTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG SMG1 D2335A/N2340K
Plasmid#199575PurposeExpresses FLAG-tagged kinase dead SMG1DepositorInsertSMG1 (SMG1 Human)
UseTagsFLAGExpressionMammalianMutationD2335A and N2340KPromoterCMVAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1371-sgGAL4
Plasmid#121514PurposesgGAL4 control. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.DepositorInsertsgGAL4
UseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromotermouse U6Available sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pHLmMBP-1
Plasmid#72343PurposeMammalian expression of secreted mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, HA-tag, Strep-tag II, and mMBPExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3_CRKL-Y207F-V5
Plasmid#196016PurposeExpresses CRKL Y207F, V5 taggedDepositorInsertCRKL
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-N2-PKCgamma
Plasmid#21204DepositorInsertPKC gamma (Prkcg Rat)
UseTagsGFPExpressionMammalianMutationPKC gamma (rat). (Lab plasmid WC 0013)PromoterAvailable sinceJuly 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
PKG I alpha CAT
Plasmid#16392DepositorInsertPKG1 alpha (PRKG1 Human)
UseTagsHAExpressionMammalianMutationCAT: catalytic domain, S65DPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCaRNA1&2
Plasmid#86087PurposeSimultaneous expression of cDNAs of complete genomic RNA1 and RNA 2 of PNRSV (Pch12 isolate) in transfected cellsDepositorInsertRNA1, RNA2
UseTagsExpressionPlantMutationPromoter35SAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCaRNA3
Plasmid#86088PurposeExpression of cDNA of full-length genomic RNA3 of PNRSV (Pch12 isolate) in transfected cellsDepositorInsertRNA3
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCold-TF-hPLEKHM1
Plasmid#64146PurposeFor protein expression in E.coliDepositorInsertPLEKHM1 (PLEKHM1 Human)
UseTags6xHis-tag and Trigger Factor (TF)ExpressionBacterialMutationPromotercspAAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMXs-ms-Sox7
Plasmid#50780Purposeretroviral expression of mouse Sox7DepositorInsertSox7 (Sox7 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
gRNA-his-HYB
Plasmid#64333Purposehis3 deletion gRNA cassette carried by pRS42HDepositorInsertgBlock product of his3 deletion gRNA cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
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Lenti-40XUAS-Luc
Plasmid#166455PurposeLuciferase reporterDepositorInsert40XUAS , Luciferase
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
B2375_CRY2olig-mEGFP
Plasmid#215109PurposeExpresses CRY2olig (E490G CRY2 1-498) fused to monomeric EGFP in mammalian cellsDepositorInsertCRY2olig-mEGFP (CRY2 Mustard Weed)
UseTagsmEGFPExpressionMammalianMutationE490G, 1-498PromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)
Plasmid#83947PurposegRNA cassette-carrying vector with natMX markerDepositorInsertgRNA targeting ADE2 gene
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IZ-ANXA4-mRuby3
Plasmid#184890PurposeStable expression of ANXA4-mRuby3 (Annexin A4-mRuby3) in mammalian cellsDepositorInsertANXA4 (ANXA4 Human)
UseTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAD-CMV-STAT5A-CA-CMV-GFP
Plasmid#83255PurposeAdenoviral expression of constitutively active STAT5A with co-expression of GFPDepositorInsertsUseAdenoviralTagsExpressionMutationconstitutively activePromoterCMVAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-p53P275S
Plasmid#22726DepositorInsertTrp53 (Trp53 Mouse)
UseRetroviralTagsExpressionMammalianMutationP275SPromoterAvailable sinceJan. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
p5E-hs_ICAM_full (JDW 1047)
Plasmid#224479PurposeGateway compatible 5' entry clone containing the Human ICAM2 promoterDepositorInsertICAM2 promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hL1
Plasmid#89411Purposemammalian expression of hL1DepositorInsertL1CAM (L1CAM Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTol2pA2-mnx1:MLS-KillerRed
Plasmid#115515PurposePlasmid driving expression of membrane bound KillerRed fluorophore in zebrafish motor neuronsDepositorInsertMLS-KillerRed
UseGateway cloningTagsMLSExpressionMutationPromoter-3mnx1Available sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Matr3 delRRM2
Plasmid#32882DepositorInsertMATR3 Matrin3 (MATR3 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationdeleted amino acids 497-566PromoterCMVAvailable sinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOneZeo-ORF18(WT)-3xFLAG
Plasmid#129746PurposeDox inducible expression of KSHV ORF18-3xFLAG in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterTRE3Gs and Tet inducible promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-Uricase
Plasmid#182401PurposeBacterial expression of Vesicle Nucleating peptide-uricase fusionDepositorInsertVNp-uricase
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMesh1
Plasmid#175594PurposeLac inducible, AMP Resistant, non labelled gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.DepositorInsertMesh1 (Mesh1 Fly)
UseTagsExpressionBacterialMutationCodon optimized for translation in E.coli. AGGAGG…PromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-FLPo-3xP3-RFP
Plasmid#127561PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.DepositorInsertFLPo
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG-SaCas9-WPRE
Plasmid#89996PurposeExpresses high levels of WT SaCas9 for genome editingDepositorInsertSaCas9 (NEWENTRY Staphylococcus aureus)
UseTags3xHA, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterCAGAvailable sinceSept. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Matr3 delRRM1
Plasmid#32881DepositorInsertMATR3 Matrin3 (MATR3 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationdeleted amino acids 399-468PromoterCMVAvailable sinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/pr-Membrane
Plasmid#120817PurposeEctopic expression of ZIKV proteinDepositorInsertpr-Membrane ZIKV
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
EC71022
Plasmid#159608PurposeLevel 0.5 Golden Gate Vector, loxP-mCHERRY-HDEL-t35s-loxPDepositorInsertloxP-mCherry-HDEL-t35S-loxP
UseCre/Lox and Synthetic BiologyTagsExpressionMutationAll BsaI, Esp3I and BPiI restriction sites were r…PromoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/EV
Plasmid#120833PurposeControl vector, 20nt random sequence between gateway recombination sitesDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tet-CA-Src-GFP
Plasmid#83469PurposeLentiviral vector for doxycycline-inducible expression of constitutively active c-Src in mammalian cellsDepositorInsertSrc (SRC Chicken)
UseLentiviralTagsEGFPExpressionMammalianMutationY527F (constitutively active)Promoterdoxycycline-inducible promoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
p8103 LentiCRISPR v2 sgNT-2
Plasmid#163313PurposeExpresses Cas9 and a non-targeting control guide RNADepositorInsertsgNT-2
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-MCPIP1 (1-599)
Plasmid#127461PurposeExpressing Flag-tagged human MCPIP1 full lengh protein in mammalian cellsDepositorInsertMCPIP1 (ZC3H12A Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a-Cas9-2XNES-2ERT2-GCN4-SV40-Zeo
Plasmid#120555PurposeExpress Cas9, 2xNES, 2 tandem ERT2 and GCN4 in mammalian cellsDepositorInsertCas9, NES, ERT2 and GCN4
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
3521 pET15b Bid p15
Plasmid#8782DepositorInsertBid p15 (Bid Mouse)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
SNRPA_Full-length_pGEX
Plasmid#135134PurposeRecombinant expression of SNRPA with dual-affinity tagDepositorInsertSNRPA (SNRPA Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceFeb. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS5
Plasmid#120825PurposeEctopic expression of ZIKV proteinDepositorInsertNS5 ZIKV
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-PKCA D463N
Plasmid#195203PurposeMammalian expression vector for GFP tagged PKCA. Kinase-inactive mutantDepositorInsertPRKCA (PRKCA Human)
UseTagsExpressionMammalianMutationD463NPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
RnBarr2-mCherry
Plasmid#137794PurposeVisualization of beta-arrestin2DepositorInsertRat beta-arrestin2 (Arrb2 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-SRT-tdTomato
Plasmid#154885PurposepiggyBac self-reporting transposon with tdTomato marker for mammalian calling cardsDepositorInsertpiggyBac SRT
UseTransposonTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-tdTomato-P2A-paCaMKII(K42M)
Plasmid#165433PurposeExpresses tdTomato and photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.DepositorInserttdTomato-P2A-paCaMKII(K42M)
UseTagstdTomatoExpressionMammalianMutationK42M: Kinase dead mutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pIRES-Ap1g1-FKBP
Plasmid#46945PurposeCo-expresses siRNA-resistant, FKBP-tagged AP-1 gamma subunit and G418 resistance gene in mammalian cellsDepositorInsertAp1g1-FKBP
UseTagsMainly mouse Ap1g1 cDNA, with a fragment of brain…ExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAXEF-Rap1-E63(GTP)
Plasmid#32698DepositorInsertRAP1 (RAP1A Human)
UseTagsExpressionMammalianMutationE63: Consitutively GTP-boundPromoterEF promoterAvailable sinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMK365 (Tet-OsTIR1-loxP-PURO-loxP)
Plasmid#121185PurposeOsTIR1 introductionDepositorInsertTet-OsTIR1-loxP-PURO-loxP
UseCRISPRTagsExpressionMutationOsTIR1 codon optimized for human cellsPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS2A
Plasmid#120818PurposeEctopic expression of ZIKV proteinDepositorInsertNS2A ZIKV
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only
