We narrowed to 25,908 results for: Nes
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Plasmid#48185PurposeExpresses CicDepositorAvailable SinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only
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pETM11-6xHis-TEV-hORF1p_R261A
Plasmid#202586PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
Tags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Arginine 261 to AlanineAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
GW1-mCherryEA
Plasmid#207867PurposeMammalian expression (cytosol) of the mCherry(I158E/Q160A) mutant as a red fluorescent pH sensorDepositorInsertmCherryEA
ExpressionMammalianPromoterCMVAvailable SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mFzd7
Plasmid#42259DepositorInsertFrizzled 7 (Fzd7 Mouse)
ExpressionMammalianAvailable SinceMarch 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJIT163-2NLSCas9
Plasmid#53064PurposeExpression of rice codon-optimized Cas9 in plant cellsDepositorInsertCas9
UsePlant expressionMutationRice codon optimizedPromoter2x35SAvailable SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lenti-DAG1
Plasmid#205149PurposeOverexpress DAG1DepositorInsertDystroglycan 1 (DAG1 Human)
UseLentiviralExpressionMammalianMutationCarries common missense variant c.41C>G (p.Ser…PromoterEF-1aAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-SP-FRB (T2098L) -sfGFP-TM-V5
Plasmid#186227PurposemRNA synthesis of rapalog-induced cell adhesion moleculeDepositorInsertmRNA synthesis of rapalog-induced cell adhesion molecule
TagsV5ExpressionMammalianMutationFRB (T2098L)Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-Txnip(C247S)
Plasmid#206353PurposeAAV plasmid expressing mutant TXNIPDepositorAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
LgBit-NbALFA
Plasmid#201484PurposeNanoBit detection of ALFA-tagged proteinDepositorInsertLgBit-NbALFA
TagsLgBitExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-HF-tetra-com-vector
Plasmid#132555PurposeVector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.DepositorInsertsHigh fidelity Cas9
sgRNA scaffold with com replacing the Tetraloop
ExpressionMammalianAvailable SinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZLblasti-CTR1
Plasmid#53157PurposeExpresses human CTR1 from blasticidin resistance retroviral vectorDepositorAvailable SinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-mIFNa2
Plasmid#135098PurposeProtein expression of mouse IFNa2 in mammalian cellsDepositorAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ubiq-Cas9.874W
Plasmid#112686PurposeExpress hSpCas9 under Ubiquitin-63E promoterDepositorInserthSpCas9
Tags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoterUbiquitin-63E promoterAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRN119
Plasmid#176884PurposeEncodes STM and WUS2 for ectopic meristem induction in plantsDepositorInsertCmYLCV:Luc+:HSP, Nos::WUS2:pinII, and 35:STM:35S
ExpressionPlantAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#113343PurposeExpresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteinsDepositorInsertp300core-dCas9-VP64-T2A-MS2-p65-HSF1
UsePiggybacAvailable SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1111
Plasmid#91032PurposeModule A, Promoter: OsAct1, Gene: TaCas9, Terminator: HSPDepositorInsertTaCas9
UseCRISPRPromoterOsAct1Available SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-mMALAT1_3' WT Sense
Plasmid#46834PurposeExpress cGFP transcript ending in the wildtype MALAT1 triple helixDepositorInsertCoral Green Fluorescent Protein (cGFP)
ExpressionMammalianPromoterCMVAvailable SinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFYF1327 EGFP Site#2
Plasmid#47512Purposehuman gRNA expression vector targeting EGFPDepositorInsertgRNA-EGFP site 2
UseCRISPRExpressionMammalianPromoterU6Available SinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEF.DEST51-mVenus
Plasmid#154899PurposeExpresses a fluorescent protein, mVenusDepositorInsertmVenus
ExpressionMammalianPromoterEF-1αAvailable SinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpyC-GFP
Plasmid#220191PurposesgRNA expression vector - pBG42 promoter with GFP in sgRNA siteDepositorInsertpBG42 promoter with GFP in sgRNA site
UseCRISPRExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG42Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
8xGliBS-IVS2-mCherry-NLS-polyA-Tol2
Plasmid#84604PurposeTol2-based hedgehog signal reporter plasmid for zebrafish transgenesisDepositorInsertmCherry
UseZebrafish transgenesisPromoter8xGliBS-delta-crystallin minimal promoterAvailable SinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_CBF-tdTomato_L3-L2
Plasmid#113708PurposeSingle pathway reporter; notch promoter-driven expression of tdTomato; MultiSite Gateway entry vector.DepositorInsertCBF (notch dependent promoter)
UseGateway CloningExpressionMammalianAvailable SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mWnt3a
Plasmid#42277DepositorInsertWnt3a (Wnt3a Mouse)
ExpressionMammalianAvailable SinceMarch 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_BAP_his
Plasmid#210402PurposeFor expression of VHH with C-terminal biotin acceptor peptide and his tagDepositorTypeEmpty backboneTagsbiotin acceptor peptide-his6ExpressionBacterialPromoterlacAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTK-EdnrbE2-mCherry
Plasmid#127772PurposeEnhancer reporter construct containing the chicken EdnrB enhancer E2 driving mCherry activityDepositorInsertednrbE2
UseEnhancer reporter constructAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMU-TGNVHAr
Plasmid#61178PurposeFluorescent reporter for trans-Golgi network AtVHA-a1-mCherry expressed under AtUBQ10 promoterDepositorInsertAtVHA-a1
TagsmCherryExpressionPlantPromoterAtUBQ10Available SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDM8 hCD81
Plasmid#11588DepositorInsertCD81 (CD81 Human)
ExpressionMammalianAvailable SinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pMC_mClover3_BSDminus_F2
Plasmid#184167PurposeDonor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmClover3
bsd
UseCRISPRAvailable SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC_mClover3_BSDminus_F1
Plasmid#184166PurposeDonor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmClover3
bsd
UseCRISPRAvailable SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE
Plasmid#201531PurposeExpressing synthetic overlapping gene, ilvA-relE. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE
UseSynthetic BiologyPromoterPrhaBAD (rhamnose)Available SinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPM1003
Plasmid#167550PurposeEncodes genes that confer expression of Vibrio cholerae O antigens of the Ogawa serotype in E. coli K-12DepositorInsertVibrio cholerae O antigens genes of the Ogawa serotype
UseCosmidAvailable SinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0103
Plasmid#91000PurposeModule A, Promoter: CmYLCV, Gene: AtCas9, Terminator: HSPDepositorInsertAtCas9
UseCRISPRPromoterCmYLCVAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Stx3
Plasmid#186685PurposeExpresses Syntaxin 3 in E. ColiDepositorInsertSyntaxin 3
Tags6 his tagExpressionBacterialAvailable SinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-M2-TLR2
Plasmid#12291DepositorInsertTLR2 (Tlr2 Mouse)
TagsFLAG (FLAG-M2)ExpressionMammalianMutationM82I and H202Y; signal peptide (aa1-23) replaced …Available SinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pETCON-PTX-S1-220
Plasmid#67747PurposePertussis Toxin Subunit 1 Yeast Display PlasmidDepositorInsertPTxS1-220
ExpressionBacterial and YeastAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMU-TGN41r
Plasmid#61176PurposeFluorescent reporter for trans-Golgi network mCherry-MtSYP41 expressed under AtUBQ10 promoterDepositorInsertMtSYP41
TagsmCherryExpressionPlantPromoterAtUBQ10Available SinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTK-EdnrbE1-Citrine
Plasmid#127773PurposeEnhancer reporter construct containing the chicken EdnrB enhancer E1 driving Citrine activityDepositorInsertednrbE1
UseEnhancer reporter constructAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Pak3(60-113)/S74A/F84A-mCherry-C1
Plasmid#29676DepositorAvailable SinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL2399
Plasmid#137051PurposeExpression of rapamycin-inducible dimerisation of split Cre from ribosomal locus in Leishmania parasite; pRIB-DiCreDepositorInsertsSplit Cre Recombinase Rapamycin Inducible Dimerisation
Blasticidin-S Deaminase
UseCre/Lox; Leishmania ribosomal locus expressionPromoterFor integration into the 18S ribosomal locusAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)
Plasmid#174529PurposeDual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiencyDepositorInsertsPylS
PylT
TagsFlagExpressionMammalianPromoterU6Available SinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFW6000
Plasmid#224215PurposeBacteroides genomic editing tool; aTc inducible-Fncpf1DepositorInsertFncpf1
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOBCol2.3-GFPemd
Plasmid#110210PurposeLabels cells in the late osteoblast lineage.DepositorInserteGFPemerald
TagsNoneExpressionMammalianPromoter2.3 kb rat type 1 collagen + first intronAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pluc2-iRFP720
Plasmid#98887PurposeMammalian expressionDepositorInsertFirefly Luciferase
TagsiRFP720ExpressionMammalianPromoterCMVAvailable SinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2092 - [1-2] ENTR - Fluor - mNeon(PATC)
Plasmid#159853PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - mNeon(PATC)
ExpressionWormMutationNot applicableAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMU-LEr
Plasmid#61185PurposeFluorescent reporter for late endosome mCherry-MtRAB5A2 expressed under AtUBQ10 promoterDepositorInsertMtRAB5A2
TagsmCherryExpressionPlantPromoterAtUBQ10Available SinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-GFP-Tdtomato-iRFP670
Plasmid#111812PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsGFP
Tdtomato
iRFP670
UseCloning intermediateAvailable SinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
LeGO-lnc-MONC
Plasmid#86966PurposeExpresses lncRNA MONC (Alias: MIR99AHG; LINC00478)DepositorAvailable SinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2 hSmad1-WT
Plasmid#22989DepositorAvailable SinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMM441
Plasmid#61271PurposeAlso referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only