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172,031 results

Showing: 11751 - 11800 of 172031 results

  5. tdTomato-Farnesyl-5

    Plasmid
    #58092
    Purpose
    Localization: Membrane, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available Since
    Dec. 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    58092-plasmid-map-sequence-id-424771

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  9. pDEST-CMV-polysite

    Plasmid
    #122843
    Purpose
    Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Type
    Empty backbone
    Use
    Gateway destination
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    122843-plasmid-map-sequence-id-244374

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  13. LV-tetO-EF1a-GFP

    Plasmid
    #187645
    Purpose
    Lentiviral vector expressing GFP from EF1a promoter downstream from tetO sites
    Depositor
    Martin Carroll
    Insert
    GFP
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187645-plasmid-map-sequence-id-367456

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  14. pENTR-CreERT2

    Plasmid
    #139547
    Purpose
    CreERT2 ORF with stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    CreERT2
    Use
    Cre/Lox
    Available Since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139547-plasmid-map-sequence-id-274672

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  20. pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3

    Plasmid
    #127353
    Purpose
    gateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and mVenus
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127353-plasmid-map-sequence-id-246311

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  21. gTBEv3

    Plasmid
    #220619
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv3) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    hUNG2-nSpCas9
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220619-plasmid-map-sequence-id-436981

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  29. pHN781

    Plasmid
    #178828
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A (AR Human)
    Tags
    His8, TEV
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178828-plasmid-map-sequence-id-351929

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  30. pGL4.82(hRluc/Puro)

    Plasmid
    #212933
    Purpose
    Renilla luciferase vector for investigating regions controlling transcription
    Depositor
    Pete Stecha
    Type
    Empty backbone
    Use
    Luciferase
    Mutation
    WT
    Available Since
    Jan. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    212933-plasmid-map-sequence-id-419194

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  33. TFORF2031

    Plasmid
    #142906
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PRDM1 (PRDM1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142906-plasmid-map-sequence-id-396079

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  41. FUGW-Arl13b-PR-EGFP-TurboID

    Plasmid
    #248194
    Purpose
    Lentiviral expression of truncated Arl13b containing the Proline-Rich domain at the C-terminus (cytosol localization)
    Depositor
    Xuecai Ge
    Article
    Liu et al Nat Commun. 2024 Apr 25;15(1):3365. doi:
    Insert
    The Arl13b noncilium targeting sequence-EGFP-TurboID (Arl13b Mouse)
    Use
    Lentiviral
    Tags
    EGFP and TurboID
    Expression
    Mammalian
    Mutation
    Truncated to only contain the PR domain
    Promoter
    hUBC
    Available Since
    Dec. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    248194-plasmid-map-sequence-id-494577

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  42. FUGW-Arl13b-N+RVEP+PR-EGFP-TurboID

    Plasmid
    #248193
    Purpose
    Lentiviral expression of truncated Arl13b containing the N-terminal amphipathic helix, cilium targeting RVEP, and the Proline-Rich domain at the C-terminus (cilium localization)
    Depositor
    Xuecai Ge
    Article
    Liu et al Nat Commun. 2024 Apr 25;15(1):3365. doi:
    Insert
    The Arl13b cilium targeting sequence-EGFP-TurboID (Arl13b Mouse)
    Use
    Lentiviral
    Tags
    EGFP and TurboID
    Expression
    Mammalian
    Mutation
    Truncated to only contain N + RVEP + PR domains
    Promoter
    hUBC
    Available Since
    Dec. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    248193-plasmid-map-sequence-id-494576

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  43. pCIOX

    Plasmid
    #51300
    Purpose
    E.coli expression vector for His8-SUMO tagged recombinant protein
    Depositor
    Andrea Mattevi
    Type
    Empty backbone
    Tags
    His8 + SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    51300-plasmid-map-sequence-id-78111

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  44. OPRD1-Tango

    Plasmid
    #66461
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    OPRD1 (OPRD1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66461-plasmid-map-sequence-id-417858

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  45. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60226
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available Since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60226-plasmid-map-sequence-id-176980

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  46. pET28b-ULP1-405-621

    Plasmid
    #229224
    Purpose
    For expression of ULP1 protein
    Depositor
    Andy LiWang
    Insert
    ULP1 (ULP1 Budding Yeast)
    Expression
    Bacterial
    Mutation
    405-621 (truncated)
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229224-plasmid-map-sequence-id-457073

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Showing: 11751 - 11800 of 172031 results