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Addgene

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159,557 results

Showing: 11751 - 11800 of 159557 results

  4. mCherry-Desmoglein2-C-18

    Plasmid
    #55031
    Purpose
    Localization: Desmosomes, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Desmoglein2 (DSG2 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55031-plasmid-map-sequence-id-450109

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  6. mPA-GFP-Actin-C-18

    Plasmid
    #57121
    Purpose
    Localization: Actin, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    Actin (ACTB Human)
    Use
    Tags
    mPA-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. pGTM_YR375_SUMO_Protease_001

    Plasmid
    #190063
    Purpose
    Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion
    Depositor
    Gaetano Montelione
    Article
    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi
    Insert
    ULP1_YEAST (ULP1 Budding Yeast)
    Use
    Tags
    8X His-MBP (Maltose Binding Protein)
    Expression
    Bacterial
    Mutation
    Construct contains residues 347 – 621
    Promoter
    T7 lac
    Available since
    Jan. 30, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    190063-plasmid-map-sequence-id-370664

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  11. mEos3.2-Vimentin-7

    Plasmid
    #57485
    Purpose
    Localization: Intermediate Filaments, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Vimentin (VIM Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. N-BLa 1.2

    Plasmid
    #88999
    Purpose
    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.
    Depositor
    Dieter Langosch
    Article
    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103
    Insert
    N-BLa 1.2 fusion protein
    Use
    Tags
    Flag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    May 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    88999-plasmid-map-sequence-id-179971

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  13. C-BLa 1.2

    Plasmid
    #89000
    Purpose
    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.
    Depositor
    Dieter Langosch
    Article
    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103
    Insert
    C-BLa 1.2 fusion protein
    Use
    Tags
    Flag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    89000-plasmid-map-sequence-id-177513

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  22. mKate2-P2A-APEX2-GBP

    Plasmid
    #67650
    Purpose
    Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.
    Depositor
    Rob Parton
    Article
    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:
    Insert
    APEX2-GBP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67650-plasmid-map-sequence-id-116990

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  24. mPA-GFP-Calnexin-N-14

    Plasmid
    #57122
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    Calnexin (CANX Human)
    Use
    Tags
    mPA-GFP
    Expression
    Mammalian
    Mutation
    I474N in Calnexin
    Promoter
    CMV
    Available since
    Jan. 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  27. pBlueKan+cysMPro

    Plasmid
    #187879
    Purpose
    Escherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.
    Depositor
    Gaylen Uhlich
    Article
    Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    CysM
    Available since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    187879-plasmid-map-sequence-id-368417

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  28. pDONR221-SLC7A5_STOP

    Plasmid
    #161363
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC7A5 (SLC7A5 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161363-plasmid-map-sequence-id-348933

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  39. mPA-GFP-Farnesyl-5

    Plasmid
    #57133
    Purpose
    Localization: Membrane, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    mPA-GFP
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    Jan. 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57133-plasmid-map-sequence-id-393243

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  43. T4 Lysozyme WT

    Plasmid
    #18110
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 26, 2008
    Availability
    Academic Institutions and Nonprofits only
    18110-plasmid-map-sequence-id-398030

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  46. pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting

    Plasmid
    #92139
    Purpose
    Targeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selection
    Depositor
    Benoit Bruneau
    Article
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Insert
    chromatin licensing and DNA replication factor 1 (GMNN, CDT1 Human)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92139-plasmid-map-sequence-id-349059

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  48. pFBOH-LIC

    Plasmid
    #26099
    Purpose
    SGC Empty baculovirus expression/transfer vector
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    6x His and Thrombin protease cleavage site
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    26099-plasmid-map-sequence-id-12255

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Showing: 11751 - 11800 of 159557 results