159,117 results

1. pET15-UbE2D1

   Plasmid

   #15782

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)
   

   Use

   Tags

   6x His, T7 tag, and Xpress tag (enterokinase clea…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Lyn FAK biosensor

   Plasmid

   #78299

   Purpose

   FRET biosensor. Visualization of FAK activity at lipid rafts in live cells

   Depositor

   Yingxiao Wang

   Article

   Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

   Insert

   Lyn Fak biosensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGF-GAS4X

   Plasmid

   #225751

   Purpose

   This plasmid was designed to selectively monitor the activation of the STAT1 signaling pathway in NK cells, a pathway known to play a crucial role in regulating NK cells' activation.

   Depositor

   Eun Hee Han

   Article

   Min et al Commun Biol. 2024 Sep 30;7(1):1222. doi:

   Insert

   interferon gamma activating sequence (GAS) (STAT1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-HA-hIFITM1

   Plasmid

   #58399

   Purpose

   Expressed human IFITM1 with an N-terminal HA tag in mammalian cells

   Depositor

   Howard Hang, Jacob Yount

   Article

   Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

   Insert

   IFITM1 (IFITM1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PKA-KTR-mStrawberry reporter

   Plasmid

   #158688

   Purpose

   This plasmid is a PKA pathway reporter. It has CMV promoter driving the expression of PKA-KTR fused to mStrawberry-pGK-BSD

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. TXNRD1

   Plasmid

   #38863

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   TXNRD1 (Txnrd1 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLKO_AU1.C.HA_mCherry-NLS

   Plasmid

   #178209

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   AU1.C.HA and Nuclear Localization Signal

   Expression

   Mutation

   Promoter

   eF1a

   Available since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FR_HNF4A2

   Plasmid

   #31100

   Depositor

   Gerhart Ryffel

   Article

   Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

   Insert

   HNF4A (HNF4A Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   splice variant 2

   Promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. non-targeting control gRNA (BRDN0001149198)

   Plasmid

   #80248

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216251

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminus

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgSEC61B.N1 (SEC61B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAJM.771

    Plasmid

    #108534

    Purpose

    NahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    NahRAM + PSalTTC-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NahR: I25V P72H V118A R248C

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-Ef1a-fDIO-eGFP-WPRE

    Plasmid

    #203843

    Purpose

    Flp-dependent EGFP expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHAGE-EF1aL-eGFP-W

    Plasmid

    #126686

    Purpose

    Constitutive, ubiquitous GFP expression.

    Depositor

    Darrell Kotton

    Article

    Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1aL

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. tol2-mpx-mCherry

    Plasmid

    #29585

    Depositor

    Anna Huttenlocher

    Article

    Yoo et al Dev Cell. 2010 Feb 16. 18(2):226-36.

    Insert

    mpx-mCherry
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pTargetF-FRT

    Plasmid

    #113637

    Purpose

    Derivative of pTargetF with gRNA targeting FRT site

    Depositor

    Olivier Lichtarge

    Article

    Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

    Insert

    sgRNA-FRT
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    pJ23119

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Arf3-GFP

    Plasmid

    #39555

    Depositor

    Paul Melancon

    Article

    Chun et al Mol Biol Cell. 2008 Aug;19(8):3488-500.

    Insert

    ADP ribosylation factor 3 (ARF3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

17. psiCHECK-2-Smad2-3'UTR

    Plasmid

    #61775

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Smad2

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Smad2 3'UTR
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pET259-pUC57 24xMS2V6

    Plasmid

    #104391

    Purpose

    MS2V6 cloning

    Depositor

    Evelina Tutucci, Robert Singer

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTRE-TIGHT miR-1 (aka:pTRE-m1d)

    Plasmid

    #14896

    Depositor

    David Bartel

    Article

    Lewis et al Cell. 2003 Dec 26. 115(7):787-98.

    Insert

    miR-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. human MMP-2 promoter

    Plasmid

    #53432

    Purpose

    promoter activity study

    Depositor

    Etty Benveniste

    Article

    Qin et al J Biol Chem. 1999 Oct 8;274(41):29130-7.

    Insert

    MMP2 promoter (MMP2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    MMP2

    Available since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET-mKelly2

    Plasmid

    #119783

    Purpose

    Expresses mKelly2 tagged N-terminally with an MHHHHHHGSG tag

    Depositor

    Stephen Mayo

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

    Insert

    mKelly2
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLKO_Ollas.S.V5_mCherry-NLS

    Plasmid

    #178211

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    Nuclear Localization Signal and Ollas.S.V5

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV5 mouse src

    Plasmid

    #13663

    Depositor

    Peter Howley, Joan Brugge

    Insert

    src (Src Mouse)
    

    Use

    Tags

    Myr

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET22b RNase A

    Plasmid

    #58903

    Purpose

    Bacterial expression of bovine pancreatic ribonuclease A

    Depositor

    Ronald Raines

    Article

    delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

    Insert

    RNase A (RNASE1 Bovine)
    

    Use

    Tags

    pelB sequence

    Expression

    Bacterial

    Mutation

    See Depositor Comments below

    Promoter

    T7

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDEST14-SpyCatcher002

    Plasmid

    #102827

    Purpose

    Bacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteins

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    SpyCatcher002
    

    Use

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKDsgRNA-FRT

    Plasmid

    #113398

    Purpose

    expresses gRNA for Cas9 FRT targetting

    Depositor

    Jan Michiels

    Article

    Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

    Insert

    exo, beta, gam, sgRNA-FRT
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBBR1k-J23107-GFPuv

    Plasmid

    #106389

    Purpose

    pBBR1k-GFPuv with Anderson promoter J23107 expressing lacI

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHD-T2A-split-intein-Gal4[1-20]-N-int

    Plasmid

    #199203

    Purpose

    To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-int

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[1-20]-N-int
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHD-T2A-split-intein-Gal4[21-881]-C-int

    Plasmid

    #199204

    Purpose

    To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[21-881]-C-int
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pQLinkHD-SYNZIP2

    Plasmid

    #80658

    Purpose

    Encodes the synthetic coiled coil peptide SYNZIP2

    Depositor

    Amy Keating

    Article

    Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

    Insert

    SYNZIP2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pQLinkHD-SYNZIP1

    Plasmid

    #80647

    Purpose

    Encodes the synthetic coiled coil peptide SYNZIP1

    Depositor

    Amy Keating

    Article

    Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

    Insert

    SYNZIP1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. TRE-UBC-H2-Kb SCT-T2A-GFP

    Plasmid

    #205454

    Purpose

    lentiviral plasmid for expression of mouse H2-Kb SCT

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    H2-Kb SCT (H2-K1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-SYN-H-sDarken

    Plasmid

    #184797

    Purpose

    High affinity version of the Serotonin Sensor sDarken under the control of a hSyn Promoter.

    Depositor

    Olivia Masseck

    Article

    Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

    Insert

    H-sDarken
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1-hSTING (N154S)

    Plasmid

    #214160

    Purpose

    Transient expression of gain-of-function STING mutant

    Depositor

    Nan Yan

    Article

    Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

    Insert

    STING (N154S) (STING1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N154S

    Promoter

    CMV

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. MMLVgag-3NES-Cre

    Plasmid

    #207252

    Purpose

    Generation of CreVLPs when used in conjunction with MMLV gag-pol during production

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    MMLVgag-3NES-Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGH224_sgRNA_2xMS2_Puro

    Plasmid

    #85413

    Purpose

    hU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-FLAG-RIPK1

    Plasmid

    #78842

    Purpose

    To overexpress RIPK1 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFB1_hMLH1

    Plasmid

    #129426

    Purpose

    Expression human MLH1 protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human MLH1 (MLH1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. plentiCMV10x-sct-pp65-b2m-HLA-A0201

    Plasmid

    #196513

    Purpose

    Express single-chain pMHC with sequencing tag for 10x genomics

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    single chain of a pp65 peptide, b2m and HLA A0201
    

    Use

    Lentiviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Y84C, 10xgenomics compatible tag

    Promoter

    CMV

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV7.1_3xFLAG-TAOK2

    Plasmid

    #172992

    Purpose

    constitutive expression of FLAG-tagged TAOK2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-TAOK2 (TAOK2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pJJiaDEST-APEX2-GAL3

    Plasmid

    #149518

    Purpose

    Express APEX2-Galectin-3 in mammalian cells

    Depositor

    Vojo Deretic

    Article

    Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Tags

    APEX2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

    Plasmid

    #85452

    Purpose

    Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

    Depositor

    Aviv Regev

    Article

    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

    Insert

    U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pRU47

    Plasmid

    #167669

    Purpose

    ENTRY vector for gRNA7 cloning under pAtU6 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU6
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Lenti-Dual-iCas9-CMV

    Plasmid

    #167500

    Purpose

    Expression of dual inducible Cas9 with CMV promoter

    Depositor

    Jake Liang

    Article

    Park et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

    Insert

    Cas9
    

    Use

    CRISPR, Lentiviral, and Luciferase

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSBbi-RB

    Plasmid

    #60522

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and blasticidin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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46. pSpCas9_human_ADAR1_ccC

    Plasmid

    #158115

    Purpose

    deletion hADAR1

    Depositor

    Emanuela Felley-Bosco

    Insert

    Adar1 (ADAR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. pEF6-Acly(S455D)

    Plasmid

    #70768

    Purpose

    Expresses phosphomimetic mouse ATP-citrate lyase in mammalian cells

    Depositor

    Kathryn Wellen

    Article

    Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

    Insert

    ATP Citrate Lyase (Acly Mouse)
    

    Use

    Tags

    myc-epitope

    Expression

    Mammalian

    Mutation

    S455D

    Promoter

    EF1alpha

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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48. pcDNA5-CEP41_Halo

    Plasmid

    #135446

    Purpose

    Mamalian cell expression of HaloTag7 localized at the centrosome

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

    Insert

    CEP41-HaloTag7
    

    Use

    Tags

    CEP41

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. pGFP

    Plasmid

    #64904

    Purpose

    "Fluorescent reporter containing no disulfide bonds/Selenocystamine folding"

    Depositor

    Stephen Mayfield

    Article

    Ferreira-Camargo et al AMB Express. 2015 Dec;5(1):126. doi: 10.

    Insert

    Green fluorescent protein
    

    Use

    Psba chlamydomonas reinhardtii vector

    Tags

    Expression

    Mutation

    Promoter

    psbA

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

50. pAAV-Ef1a-fDIO-mRuby3-2A-Cre

    Plasmid

    #203844

    Purpose

    Flp-dependent Cre and mRuby3 expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    Cre
    

    Use

    AAV

    Tags

    mRuby3-2A

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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