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177,138 results

Showing: 11801 - 11850 of 177138 results

  1. pH6-MBP-TEV-BirA

    Plasmid
    #179694
    Purpose
    Expresses His6 tagged MBP-BirA fusion protein
    Depositor
    Mark Arbing
    Insert
    BirA
    Tags
    His6-MBP
    Expression
    Bacterial
    Available since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    179694-plasmid-map-sequence-id-352195

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  2. pGP-pcDNA3.1 Puro-CAG-Voltron2-ST

    Plasmid
    #172910
    Purpose
    Mammalian expression of voltage sensor; soma localization targeting sequence
    Depositor
    GENIE Project
    Publication
    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Voltron2-ST
    Tags
    soma localization targeting sequence
    Expression
    Mammalian
    Mutation
    A122D
    Promoter
    CAG
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    172910-plasmid-map-sequence-id-341196

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  3. pcDNA3-pmEKAR4

    Plasmid
    #174440
    Purpose
    Plasma membrane-targeted EKAR4.
    Depositor
    Jin Zhang
    Publication
    Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pmEKAR4
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    174440-plasmid-map-sequence-id-344974

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  4. pOpen-phi29pol

    Plasmid
    #165573
    Purpose
    DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    phi29 DNA Polymerase
    Use
    Synthetic Biology
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165573-plasmid-map-sequence-id-340935

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  5. pOpen-MMLV_RT (lack H)

    Plasmid
    #165556
    Purpose
    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
    Use
    Synthetic Biology
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165556-plasmid-map-sequence-id-339910

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  7. pOpen-GBDpol

    Plasmid
    #165501
    Purpose
    Robust and extremely thermostable polymerase with 5x higher fidelity than Taq. Ideal for GC-rich or looped sequences.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Pyrococcus Sp. Heat-Stable DNA Polymerase
    Use
    Synthetic Biology
    Available since
    July 26, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165501-plasmid-map-sequence-id-338429

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  12. pLSB-KAN

    Plasmid
    #166700
    Purpose
    Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Publication
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA Ser promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    166700-plasmid-map-sequence-id-329224

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  13. pGY577

    Plasmid
    #166663
    Purpose
    Light-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombination
    Depositor
    Gaël Yvert
    Publication
    Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:
    Insert
    LiCre
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Mutation
    Cre: E340A, D341A
    Promoter
    CMV
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    166663-plasmid-map-sequence-id-330243

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  21. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE

    Plasmid
    #162381
    Purpose
    AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Has service
    AAV1, AAV5, AAV9, and AAV Retrograde
    Insert
    jGCaMP8m
    Use
    AAV and Cre/Lox
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    A25G F286Y
    Promoter
    CAG
    Available since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    162381-plasmid-map-sequence-id-413019

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  24. pUltra-Plus-H2B-mTagBFP2

    Plasmid
    #161790
    Purpose
    3rd generation lentiviral bicistronic vector for mammalian expression of H2B-mTagBFP2 and one gene of interest
    Depositor
    Jorge Luis Arias-Arias
    Insert
    H2B-mTagBFP2
    Use
    Lentiviral; Bicistronic
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    I174A in the mTagBFP2 protein according to PLoS O…
    Promoter
    UbC and UbC promoter
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161790-plasmid-map-sequence-id-316820

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  29. pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP

    Plasmid
    #155307
    Purpose
    Rfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFP
    Depositor
    Stanley Qi
    Publication
    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do
    Insert
    Rfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    hU6, EF1a
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    155307-plasmid-map-sequence-id-442982

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  34. pCAGGs-Venus

    Plasmid
    #127346
    Purpose
    Expresses Venus constitutively via the CAGGS promoter in mammalian cells
    Depositor
    Phil Sharp
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus Fluorescent Protein
    Expression
    Mammalian
    Promoter
    CAGGS Promoter
    Available since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    127346-plasmid-map-sequence-id-246571

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  42. Fibronectin-human-EDA in pMAX

    Plasmid
    #120402
    Purpose
    enables eukaryotic expression of human EDA (or EIIIA) containing fibronectin based on plasma fibronectin sequence
    Depositor
    Inaam Nakchbandi
    Publication
    Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EDA-fibronectin (FN1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    120402-plasmid-map-sequence-id-251099

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  43. pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE

    Plasmid
    #125256
    Purpose
    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by EF1a promoter for optogenetic activation with puro selection
    Depositor
    Roger D. Kamm
    Publication
    Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi
    Insert
    hChR2(H134R)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    EF1a-forward
    Available since
    May 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    125256-plasmid-map-sequence-id-245091

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  49. pORTMAGE311B

    Plasmid
    #120418
    Purpose
    Expresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanR
    Depositor
    Csaba Pál
    Publication
    Szili et al Antimicrob Agents Chemother. 2019 Aug 23
    Inserts
    MutL E32K
    Lambda Beta
    XylS
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    Pm
    Available since
    Feb. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    120418-plasmid-map-sequence-id-236013

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Showing: 11801 - 11850 of 177138 results