164,220 results

1. pFA6a-6xGLY-V5-HIS3MX6

   Plasmid

   #20777

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-EGFP-Cdc42(T17N)

   Plasmid

   #12601

   Depositor

   Klaus Hahn

   Article

   Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

   Insert

   EGFP-Cdc42 (T17N) (CDC42 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T17N

   Promoter

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. LeGO-EBFP2

   Plasmid

   #85213

   Purpose

   Lentiviral vector expressing EBFP2 fluorescent protein driven by SFFV promoter

   Depositor

   Boris Fehse

   Article

   Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

   Insert

   EBFP2
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRDA_886

   Plasmid

   #201162

   Purpose

   Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. WT NFAT2

   Plasmid

   #11101

   Depositor

   Anjana Rao

   Article

   Monticelli et al Eur J Immunol. 2002 Oct . 32(10):2971-8.

   Insert

   NFAT2 (Nfatc1 Mouse)
   

   Use

   Retroviral

   Tags

   3xHA and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-cox8-iPASnFR-L-mScarlet3-3xFLAG

   Plasmid

   #225240

   Purpose

   Mammalian expression of iPASnFR-L in mitochondria

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-L
   

   Use

   Tags

   3xFLAG and mScarlet3

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZac2.1 gfaABC1D-tdTomato

   Plasmid

   #44332

   Depositor

   Baljit Khakh

   Article

   Shigetomi et al J Gen Physiol. 2013 Apr 15.

   Has Service

   AAV5

   Insert

   tdTomato
   

   Use

   AAV; Adeno-associated virus ()

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPSU20

   Plasmid

   #173829

   Purpose

   Expresses 20 kD Penn State ladder protein

   Depositor

   Song Tan

   Article

   Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

   Insert

   STRHSTPABPACCBPv3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV3Tag8li-hADARB2-3xFLAG

   Plasmid

   #165470

   Purpose

   Human ADARB2 mammalian expression vector with 3 C-terminal FLAG tags.

   Depositor

   Martin Dorf

   Article

   Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

   Insert

   Adenosine deaminase RNA specific B2 (inactive) (ADARB2 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSIN4-EF2-O2S

    Plasmid

    #21162

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Oct4, Sox2 (SOX2, POU5F1 Human)
    

    Use

    Lentiviral; Reprogramming

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-FLAG-CaMKIIA

    Plasmid

    #186825

    Purpose

    Expresses FLAG-CaMKIIA in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    Camk2a (Camk2a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV4-p52

    Plasmid

    #23289

    Depositor

    Warner Greene

    Article

    Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

    Insert

    p52 (NFKB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNCS-mCyRFP1

    Plasmid

    #84545

    Purpose

    Bacterial expression of mCyRFP1

    Depositor

    Michael Lin

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mCyRFP1
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-15F11-HA-Halo

    Plasmid

    #129592

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZac2.1 gfaABC1D-lck-GCaMP6f

    Plasmid

    #52924

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Baljit Khakh

    Has Service

    AAV5

    Insert

    lck-GCaMP6f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mutation

    Promoter

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. LVXN-Neo-NSD2-E1099K

    Plasmid

    #86011

    Purpose

    express NSD2 E1099K mutant in mammalian cells

    Depositor

    Darrin Stuart

    Article

    Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

    Insert

    NSD2 E1099K mutant (NSD2 Human)
    

    Use

    Tags

    Flag tag  D Y K D D D D K

    Expression

    Mammalian

    Mutation

    E1099K; K1002R (please see depositor comments bel…

    Promoter

    CMV

    Available Since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

17. TRE:mCherry-p2a- bReaChES

    Plasmid

    #163037

    Purpose

    FLiCRE reporter gene for neuronal expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    mCherry-p2a-bReaChES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET30b-7+-SrtA

    Plasmid

    #105602

    Purpose

    expressed 7+ sortase A variant in E.coli

    Depositor

    Stephanie Dougan

    Article

    Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

    Insert

    S.aureus Sortase A, 7+ mutant
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pACE-GIRK2-S

    Plasmid

    #172426

    Purpose

    Expression of full-length, human GIRK2 with C-terminal StrepTag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

    Insert

    GIRK2 (KCNJ6 Human)
    

    Use

    Tags

    Streptag II

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-TRE-ChR2-YFP

    Plasmid

    #171622

    Purpose

    TRE-ChR2-YFP reporter

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    TRE-ChR2-YFP
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDH-NLS-copGFP-EF1-BlastiS

    Plasmid

    #132772

    Purpose

    Spills into the cytoplasm during nuclear envelope rupture.

    Depositor

    Jan Lammerding

    Article

    Denais et al Science. 2016 Apr 15;352(6283):353-8. do

    Insert

    NLS-copGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFPC1-hVAP-A

    Plasmid

    #104447

    Purpose

    Expression of human VAP-A fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein A (VAPA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti PGK GFP Puro (w509-5)

    Plasmid

    #19070

    Purpose

    3rd gen lentiviral eGFP expression vector, PGK promoter, Puro 

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJMP3665

    Plasmid

    #222351

    Purpose

    Expression vector with PabstBR promoter, hygR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

    Depositor

    Jason Peters

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LT3GEP

    Plasmid

    #111172

    Purpose

    Tet-regulated miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    T3G

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV.CAG.GCaMP6s.WPRE.SV40

    Plasmid

    #100844

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

    Promoter

    CAG

    Available Since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pInt

    Plasmid

    #18941

    Depositor

    Michele Calos

    Article

    Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

    Insert

    phiC31 integrase (int Streptomyces phage phiC31)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    under lacZ promoter

    Promoter

    Available Since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGLO-Magenta-AZA

    Plasmid

    #220784

    Purpose

    Expresses a magenta chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)

    Depositor

    Angela Leu

    Insert

    magenta chromoprotein
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJV53

    Plasmid

    #26904

    Depositor

    Graham Hatfull

    Article

    van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

    Insert

    Che9c genes 60-61
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLPCX mito roGFP2-Orp1

    Plasmid

    #64992

    Purpose

    Mammalian expression of mitochondrial roGFP2-Orp1 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    mitochondrial targeting sequence

    Promoter

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1-FGFR1c-V5/HIS

    Plasmid

    #201106

    Purpose

    expression of human FGFR1 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human FGFR1 receptor tyrosine kinase, variant c, full length, wildtype (FGFR1 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pH2B-PSmOrange2

    Plasmid

    #34960

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al J Am Chem Soc. 2012 Aug 17.

    Insert

    PSmOrange2
    

    Use

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    R17H, R36H, F64I, Q194L, A224S, G226A (mutations …

    Promoter

    Available Since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. TRE-mCherry-p2A-eNpHR3.0-TS

    Plasmid

    #158703

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    mCherry-p2A-eNpHR3.0-TS
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pB18cmBFPAzurite

    Plasmid

    #14034

    Depositor

    Patrick Daugherty

    Article

    Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

    Insert

    Azurite
    

    Use

    Yeast/bacteria optimized codons

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pETMCSI-T4P_Y320A

    Plasmid

    #122522

    Purpose

    Expression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesis

    Depositor

    Gottfried Otting

    Article

    Qi et al PLoS One. 2019 Jan 23;14(1):e0211065. do

    Insert

    gp43 - DNA polymerase (43 Escherichia virus T4)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    tyrosine 320 changed to alanine

    Promoter

    T7 promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDV052

    Plasmid

    #191825

    Purpose

    Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpR

    Depositor

    Jim Roberts

    Article

    Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDONR221_SLC3A2

    Plasmid

    #132213

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC3A2 (SLC3A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pFUS-WT-VC

    Plasmid

    #175068

    Purpose

    expresses FUS wildtype tagged to mVenus and mCherry in mammalian cells

    Depositor

    Arnold Boersma

    Article

    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

    Insert

    FUS glycine rich protein (FUS Human)
    

    Use

    Tags

    HA and mVenus + mCherry

    Expression

    Mammalian

    Mutation

    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

    Promoter

    CMV Promoter

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. GAL4UAS-Luciferase reporter

    Plasmid

    #64125

    Purpose

    Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    GAL4UAS
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    GAL4UAS

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TurboID-V5_pRS415

    Plasmid

    #107167

    Purpose

    expresses V5-tagged TurboID in the yeast cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge