We narrowed to 6,529 results for: nls

Showing: 961 - 1000 of 6529 results

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pINO4-NLS-Pfv-Sapphire-HO

Plasmid

#203660

Purpose

Yeast vector encoding donor DNA for integration of nuclear 40nm GEMs into the HO locus.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Use

CRISPR

Tags

Sapphire

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-2xNLS-mTurquoise2

Plasmid

#104053

Purpose

An AAV genome encoding expression of the nuclear localized fluorescent protein mTurquoise2 from the GFAP promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

2xNLS-mTurquoise2

Use

AAV

Tags

NLS

Promoter

GFAP

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE-NLS-HA-TurboID-PCNA

Plasmid

#215074

Purpose

Expresses NLS-HA-TurboID-PCNA in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

PCNA (PCNA Human)

Use

Retroviral

Tags

NLS-HA-TurboID

Expression

Mammalian

Promoter

LTR

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-BG4-NLS-mVen

Plasmid

#211449

Purpose

mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence

Depositor

Helmut Pospiech

Insert

Anti-DNA G-quadruplex single chain antibody BG4

Tags

NLS-mVenus

Expression

Mammalian

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS613_pTetR-P2A-BFPnls/sgTelo

Plasmid

#108649

Purpose

Telomere-targeting Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Telomere-targeting sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-PCP-GFPnls

Plasmid

#121938

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

PCP-GFPnls

Use

Lentiviral

Tags

sfGFP

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-N22p-3XRFPnls

Plasmid

#75387

Purpose

N22p-3XRFPnls

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

N22p

Use

Lentiviral

Tags

3XmCherry

Expression

Mammalian

Promoter

EFS

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-EGFP

Plasmid

#220594

Purpose

Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

EGFP

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-PCP-3XGFPnls

Plasmid

#75385

Purpose

PCP-3XGFPnls

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

PCP

Use

Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

EFS

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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PET-B-HeFSpCas9-NLS-6xHis

Plasmid

#207392

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A…

Promoter

T7

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a-3NLS-NGN2TALE-GCN4

Plasmid

#120547

Purpose

Express 3xNLS-NGN2 TALE-GCN4 engineered to bind a site in the human NGN2 ene

Depositor

Yu Wang

Article

Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

Insert

NLS, NGN2 TALE, GCN4 (NEUROG2 Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1α

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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NLS-GFP-iLID-Ferritin

Plasmid

#227376

Purpose

Condensate module, light-inducible oligomerization 'core'

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

GFP 'core'

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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764 pME nls-mApple

Plasmid

#195962

Purpose

Gateway pME fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mApple nuclear

Use

Other

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pZLCv2-3xFLAG-dCas9-HA-2xNLS

Plasmid

#106357

Purpose

Empty backbone for CRISPR/Cas9 engineered chromatin immunoprecipitation

Depositor

Stephen Tapscott

Article

Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

3XFLAG and HA

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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765 pME nls mCerulean

Plasmid

#195958

Purpose

Gateway pME fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mCerulean nuclear

Use

Other

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDY0738 pCAG-NLS-SscINT

Plasmid

#179113

Purpose

SscINT expression vector

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCAG-NLS-SscINT

Use

CRISPR

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDY0739 pCAG-NLS-SacINT

Plasmid

#179114

Purpose

SacINT expression vector

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCAG-NLS-SacINT

Use

CRISPR

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-Peredox-mCherry-NLS

Plasmid

#32385

Depositor

Gary Yellen

Article

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

Peredox-mCherry-NLS

Use

Retroviral

Tags

Nuclear Localization Seq

Expression

Mammalian

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-His:dCas9:nls:10P3

Plasmid

#135982

Purpose

Expresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

dCas9 fused to 10 copies of the P3 coiled-coil

Use

CRISPR

Tags

His

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-mKate2

Plasmid

#220599

Purpose

Constitutive expression of a single-cell discriminating version of mKate2 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

mKate2

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E6-dTom-nlsdTom

Plasmid

#135641

Purpose

AAV vector to drive the expression of dTomato in VIP cortical interneurons under the control of the E6 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV1

Insert

dTom-nlsdTom

Use

AAV

Mutation

None

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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ptagBFP-LacI-NLS-VP16

Plasmid

#103837

Purpose

Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

tagBFP-LacI-NLS-VP16

Expression

Mammalian

Promoter

SV2

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHsp83-NLS-HA-2xMCP-2xTagRFP-T

Plasmid

#71242

Purpose

Used to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.

Depositor

Anne Ephrussi

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

NLS-2xMCP-2xTagRFP-T

Use

P element-mediated germline transformation

Tags

TagRFP-T

Expression

Insect

Promoter

Hsp83

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCW57.1-3xFLAG-NLS-GFP

Plasmid

#198238

Purpose

Lentivector with tet-inducible 3xFLAG-NLS-GFP

Depositor

Stephen Tapscott

Article

Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

Insert

3xFLAG-NLS-GFP

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV2-CAG-3xNLS-vsfGFP-0

Plasmid

#191097

Purpose

To express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

Depositor

Murray Blackmore, Pantelis Tsoulfas

Article

Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

Insert

3xNLS-vsfGFP-0

Use

AAV

Tags

Green fluorescent protein bound to enhancer nanob…

Expression

Mammalian

Mutation

Optimized to Human codon usage

Promoter

CMV and CAG

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDonor-tBFP-NLS-Neo

Plasmid

#80766

Purpose

Customizable donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Type

Empty backbone

Use

CRISPR

Tags

TagBFP2-3×NLS

Expression

Mammalian

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-BG4-NLS-mCer

Plasmid

#211448

Purpose

mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence

Depositor

Helmut Pospiech

Insert

Anti-DNA G-quadruplex single chain antibody BG4

Tags

NLS-mCerulean

Expression

Mammalian

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GW1-Peredox-mCherry-NLS

Plasmid

#32381

Depositor

Gary Yellen

Article

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

Peredox-mCherry-NLS

Tags

Nuclear Localization Seq

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN019-SpCas9-hiNLS-t-M1M4

Plasmid

#234456

Purpose

Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

Depositor

Ross Wilson

Article

Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

Insert

SpCas9 hiNLS t-M1M4

Use

CRISPR

Tags

His6, Nucleoplasmin, SV40, and c-Myc

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
PET-eSpCas9-NLS-6xHis

Plasmid

#207379

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

eSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, K1003A, R1060A

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag-NLS-eGFP-SV40

Plasmid

#117811

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Tags

NLS

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-MBP-NLS-Geo_st

Plasmid

#87703

Purpose

Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS

Depositor

Jennifer Doudna

Insert

GeoCas9

Tags

10xHis-MBP-TEV

Expression

Bacterial

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC-fFuCas9-HTBNLS-hph

Plasmid

#121092

Purpose

Multigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 system

Depositor

Xiao-Jun Ji

Article

Shi et al ACS Synth Biol. 2019 Feb 15;8(2):445-454

Insert

Cas9 and hygromycin resist gene

Use

CRISPR

Expression

Bacterial

Promoter

gpdA;trpC

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-TetR-NLS-VP16

Plasmid

#103834

Purpose

Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

EGFP-TetR-NLS-VP16 (tetR )

Expression

Mammalian

Promoter

SV2

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-RAB-EKARev-NLS

Plasmid

#118476

Purpose

ddRFP-based single-color ERK biosensor targeted to the nucleus.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev-NLS

Tags

6xHIS, Nuclear localization signal (NLS), T7 tag …

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
UbC NLS-HA-MCP-YFP

Plasmid

#31230

Depositor

Robert Singer

Article

Grunwald et al Nature. 2010 Sep 30. 467(7315):604-7.

Insert

NLS-HA-MCP-YFP

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-FNLSNG-P2A-Puro

Plasmid

#136900

Purpose

Lentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

FNLSNG

Use

Lentiviral

Tags

FLAG-NLS and NLS

Mutation

L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337R

Promoter

EF1s

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-Octo_VirD2_NLS-Cas9-6xHis

Plasmid

#232296

Purpose

Expresses Cas9 protein fused with octopine-type VirD2-originating NLS

Depositor

Hee-Sik Kim

Article

Le et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

Insert

Octo_VirD2_NLS-Cas9-6xHis

Use

CRISPR

Tags

6xHis and Octopine-type VirD2-derived NLS

Expression

Bacterial

Promoter

T7

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-NLS-tdMCP-eGFP

Plasmid

#86243

Purpose

Middle entry vector for inserting promotors for tissue specific expression of NLS-tdMCP-eGFP reporter

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Type

Empty backbone

Use

Unspecified

Tags

NLS-tdMCP-eGFP

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-CMV-TO-EGFRvIII-IRES-nlsChe

Plasmid

#116039

Purpose

Supporting plasmid for testing protease circuits

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

EGFRvIII-IRES-nlsChe

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 6,529 results for: nls