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Showing: 10761 - 10800 of 23940 results

  8. Lenti-DAG1

    Plasmid
    #205149
    Purpose
    Overexpress DAG1
    Depositor
    Monkol Lek
    Insert
    Dystroglycan 1 (DAG1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Carries common missense variant c.41C>G (p.Ser…
    Promoter
    EF-1a
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    205149-plasmid-map-sequence-id-405998

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  14. pJMP8930

    Plasmid
    #220905
    Purpose
    MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PC-dCas9-novo (for cloning new guides)
    Depositor
    Jason Peters
    Article
    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003
    Insert
    dCas9-novo
    Use
    CRISPR
    Tags
    3xMyc
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220905-plasmid-map-sequence-id-436985

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  17. pBS SK mCherryROSAegfp

    Plasmid
    #54322
    Purpose
    Encodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR.
    Depositor
    Silvestro Conticello
    Article
    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.
    Insert
    mCherry and EGFP
    Use
    CRISPR, Mouse Targeting, and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    54322-plasmid-map-sequence-id-84459

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  29. pCas3

    Plasmid
    #229538
    Purpose
    pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Insert
    Cas3
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae
    Promoter
    Available since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229538-plasmid-map-sequence-id-459007

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  34. pDual-eGFP

    Plasmid
    #63215
    Purpose
    Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 10761 - 10800 of 23940 results