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We narrowed to 24,790 results for: SPR

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Showing: 1081 - 1120 of 24790 results

  9. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid
    #138147
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NLS
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138147-plasmid-map-sequence-id-349848

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  10. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

    Plasmid
    #138148
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NES
    Available Since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    138148-plasmid-map-sequence-id-318593

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  11. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

    Plasmid
    #85756
    Purpose
    All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Article
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Inserts
    Csy4-T2A-SpRFN-P2A-GFP
    gRNA
    Use
    CRISPR
    Tags
    Csy4, Csy4 recognition sequence, FokI fusion via …
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CMV and U6
    Available Since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85756-plasmid-map-sequence-id-170275

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  15. pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE

    Plasmid
    #138143
    Purpose
    Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    PguCas13b
    Use
    Lentiviral
    Tags
    NLS
    Available Since
    March 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138143-plasmid-map-sequence-id-275400

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  16. pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE

    Plasmid
    #138146
    Purpose
    Expresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caac
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    PspCas13b
    Use
    Lentiviral
    Tags
    NES
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138146-plasmid-map-sequence-id-272533

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  17. pCMV-T7-BPNLS-CE-ABE8e-SpRY--BPNLS-P2A-EGFP (NK289)

    Plasmid
    #208293
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpRY A-to-G base editor and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-CE-ABE8e-SpRY-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA inlaid into nSpRY(D10A/A6…
    Promoter
    CMV and T7
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208293-plasmid-map-sequence-id-410845

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  18. pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE

    Plasmid
    #138144
    Purpose
    Expresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    PguCas13b
    Use
    Lentiviral
    Tags
    NES
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138144-plasmid-map-sequence-id-272529

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  31. pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP (CA136)

    Plasmid
    #208291
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpRY(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations and V106W in TadA and nSpRY(D10A/…
    Promoter
    CMV and T7
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208291-plasmid-map-sequence-id-410846

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  32. pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)

    Plasmid
    #185491
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185491-plasmid-map-sequence-id-362675

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  36. pCMV-T7-nSpRY(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES395)

    Plasmid
    #185489
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpRY(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpRY(H840A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    H840A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N13…
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185489-plasmid-map-sequence-id-362674

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  37. pLentiRNACRISPR_012-TetO-NLS-inactive-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid
    #228558
    Purpose
    Expresses inactive hfCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus
    Depositor
    Neville Sanjana
    Article
    Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103
    Insert
    dhfCas13d (N2V8)
    Use
    Lentiviral
    Tags
    NLS-HA
    Mutation
    R239A/H244A/R858A/H863A
    Available Since
    Aug. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    228558-plasmid-map-sequence-id-457180

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Showing: 1081 - 1120 of 24790 results