Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 22,836 results for: Sis

Showing: 11281 - 11320 of 22836 results

  10. pHYG

    Plasmid
    #243678
    Purpose
    Expresses the HYGROMYCIN PHOSPHOTRANSFERASE (HPT) CDS in plants using the Cestrum Yellow Leaf Curling Virus (CmYLCV) 9.11 promoter and an Arabidopsis HSP terminator
    Depositor
    Patrick Krysan
    Article
    De La Mora et al Plant Cell Rep. 2025 Oct 19;44(11):243.
    Insert
    HYGROMYCIN PHOSPHOTRANSFERASE (HPT) CDS
    Expression
    Plant
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    243678-plasmid-map-sequence-id-491547

    Enlarge

  12. p305N-9 (nonfunctional)

    Plasmid
    #246292
    Purpose
    Evaluation of AtU6.29c13 promoter (nonfunctional) (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    AtU6.29c13 promoter (nonfunctional)
    Use
    CRISPR
    Expression
    Plant
    Mutation
    deletions: -A (-58 from TSS), -T (-57) within USE
    Promoter
    AtU6.29c13 promoter (nonfunctional)
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246292-plasmid-map-sequence-id-491207

    Enlarge

  13. p305N-17 (nonfunctional)

    Plasmid
    #246300
    Purpose
    Evaluation of HbU6.2m3 promoter (nonfunctional) (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    HbU6.2m3 promoter (nonfunctional)
    Use
    CRISPR
    Expression
    Plant
    Mutation
    C-to-T (-29 from TSS), G-to-A (-24)
    Promoter
    HbU6.2m3 promoter (nonfunctional)
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246300-plasmid-map-sequence-id-491174

    Enlarge

  14. p305N-4 (nonfunctional)

    Plasmid
    #246287
    Purpose
    Evaluation of AtU6.1c1 promoter (nonfunctional) (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    AtU6.1c1 promoter (nonfunctional)
    Use
    CRISPR
    Expression
    Plant
    Mutation
    G-to-C at -15 from TSS
    Promoter
    AtU6.1c1 promoter (nonfunctional)
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246287-plasmid-map-sequence-id-491143

    Enlarge

  23. mE-mCherry2A

    Plasmid
    #236248
    Purpose
    Middle entry fluorescent mCherry reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separately
    Depositor
    James Amatruda, Genevieve Kendall
    Article
    Kendall et al eLife. 2018 Jun 5;7. pii: 33800. doi: 10
    Insert
    mCherry2A
    Expression
    Mammalian
    Mutation
    Added a viral2A sequence to mCherry with no stop …
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    236248-plasmid-map-sequence-id-486041

    Enlarge

  24. mE-GFP2A

    Plasmid
    #236247
    Purpose
    Middle entry fluorescent GFP reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separately
    Depositor
    James Amatruda, Genevieve Kendall
    Article
    Kendall et al eLife. 2018 Jun 5;7. pii: 33800. doi: 10
    Insert
    eGFP
    Expression
    Mammalian
    Mutation
    Added a viral2A sequence with no stop codon
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    236247-plasmid-map-sequence-id-486040

    Enlarge

  26. pcDNA3.1-NES-CAGEc-AF9c (Nrdj1)

    Plasmid
    #241414
    Purpose
    Proximity-triggered Protein Trans-Splicing to generate the splice product MLL-AF9, Figure 2 & Extended Figure 4 in Nature Chemical Biology 20.10 (2024): 1353-1360.
    Depositor
    Thomas Muir
    Article
    Lee et al bioRxiv [Preprint]. 2023 Jul 10:2023.07.
    Insert
    NES-CAGEc-AF9c (Nrdj1) (MLLT3 Synthetic, Human)
    Tags
    3xFLAG and HA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    241414-plasmid-map-sequence-id-485484

    Enlarge

  27. pEmal10H – MBP-EWS_300-447 (RGG1+RRM)

    Plasmid
    #238369
    Purpose
    Expresses the RGG1-RRM (300-447) region from the RNA-binding protein EWS
    Depositor
    David Libich
    Insert
    EWSR1 (EWSR1 Human)
    Tags
    10xHis-MBP
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-299, 448-638
    Promoter
    T7
    Available Since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    238369-plasmid-map-sequence-id-478743

    Enlarge

  28. pEmal10H – MBP-EWS_549-638 (RGG3)

    Plasmid
    #238370
    Purpose
    Expresses the RGG3 (549-638) region from the RNA-binding protein EWS
    Depositor
    David Libich
    Insert
    EWSR1 (EWSR1 Human)
    Tags
    10xHis-MBP
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-548
    Promoter
    T7
    Available Since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    238370-plasmid-map-sequence-id-478744

    Enlarge

  29. pUC19_21 tRNA genes

    Plasmid
    #242430
    Purpose
    In vitro transcription of 21 tRNAs after Nt.BspQI treatment. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene.
    Depositor
    Sebastian Maerkl
    Article
    Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:
    Insert
    21 tRNA genes with a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene
    Expression
    Bacterial
    Available Since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    242430-plasmid-map-sequence-id-485004

    Enlarge

  32. pmEos3.1-N1 GBP

    Plasmid
    #220492
    Purpose
    Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos3.1 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy
    Depositor
    Frederic Meunier
    Article
    Gormal et al Nat Protoc. 2025 Aug 8. doi: 10.1038/s41
    Insert
    GFP Binding Protein tagged with mEos3.1
    Tags
    mEos3.1
    Expression
    Mammalian
    Available Since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    220492-plasmid-map-sequence-id-442019

    Enlarge

  33. pGTPA-Gm

    Plasmid
    #171527
    Purpose
    Plasmid contains the Cas12k gRNA and transposon donor cassette assembled with gentamycin-resistant gene
    Depositor
    Quanjiang Ji
    Article
    Chen et al Cell Rep. 2021 Aug 31;36(9):109635. doi:
    Insert
    Cas12k gRNA, transposon donor cassette, gentamycin-resistant gene
    Use
    CRISPR
    Promoter
    Constitutive promoter J23119
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    171527-plasmid-map-sequence-id-340981

    Enlarge

  35. pEPHS1CB0027

    Plasmid
    #227570
    Purpose
    Level 1 - Position 1 with 35S promoter and terminator for plant expression, with mutations in CDS
    Depositor
    Nicola Patron
    Article
    Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:
    Insert
    35Sshort_TMV_TkTXSS (T374G/E379D/Q486H/A745P)_35S
    Expression
    Plant
    Mutation
    T374G / E379D / Q486H / A745P, BsaI sites removed…
    Promoter
    35Sshort_TMV (pICH51277)
    Available Since
    July 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    227570-plasmid-map-sequence-id-455283

    Enlarge

  36. pHS1625

    Plasmid
    #239834
    Purpose
    OrufIscB-REC E. coli expression for recombinant purification
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    OrufIscB-REC
    Tags
    14xHis-TwinStrep-bdSUMO
    Expression
    Bacterial
    Mutation
    Insertion of REC domain from NbaCas9-1
    Promoter
    T7
    Available Since
    July 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    239834-plasmid-map-sequence-id-479361

    Enlarge

  40. pHS1742

    Plasmid
    #239838
    Purpose
    OrufIscB-KRK mammalian expression
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    OrufIscB-KRK
    Tags
    Nucleoplasmin NLS-3xHA and SV40 NLS
    Expression
    Mammalian
    Mutation
    Insertion of REC domain from NbaCas9-1 + E137K/E4…
    Promoter
    CMV
    Available Since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    239838-plasmid-map-sequence-id-479115

    Enlarge

Showing: 11281 - 11320 of 22836 results