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Showing: 101 - 150 of 664 results

  8. HF-PX459 (V2)

    Plasmid
    #118632
    Purpose
    Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance
    Depositor
    Mike McGrew
    Article
    Idoko-Akoh et al Sci Rep. 2018 Oct 11;8(1):15126. doi: 10
    Insert
    hSpCas9-HF1-2A-Puro V2.0
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Mutation
    Asparagine 497 to Alanine, Arginine 661 to Alanin…
    Promoter
    Cbh
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    118632-plasmid-map-sequence-id-230217

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  9. ROSA26(MultiFPsΔPuro)

    Plasmid
    #140759
    Purpose
    Targeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).
    Depositor
    Hideyuki Okano
    Article
    Serizawa et al Development. 2019 Nov 4;146(21). pii: de
    Inserts
    puromycin-N-acetyltransferase
    Venus
    mCherry
    mCerulean
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    CAGGS
    Available since
    June 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140759-plasmid-map-sequence-id-311014

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  12. FOR human NANOS3 TALEN

    Plasmid
    #59722
    Purpose
    TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)
    Depositor
    Jacob Hanna
    Article
    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:
    Insert
    HD HD NG NH HD NG HD NG HD HD HD NG HD HD NI NG
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGGS
    Available since
    Dec. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. REV human NANOS3 TALEN

    Plasmid
    #59723
    Purpose
    TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)
    Depositor
    Jacob Hanna
    Article
    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:
    Insert
    NG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGGS
    Available since
    Dec. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  25. pnos-Fok1:dCas9-nos

    Plasmid
    #62210
    Purpose
    Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.
    Depositor
    Simon Bullock
    Insert
    Fok1-dCas9
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    nanos
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    62210-plasmid-map-sequence-id-101869

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  48. pcs2-cDELE1(142-515)-sumo

    Plasmid
    #226107
    Purpose
    cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) fused to sumo used for in-vitro translation by wheat germ extract in in-vitro ubiquitylation assays
    Depositor
    Michael Rape
    Article
    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:
    Insert
    DELE1 (DELE1 Human)
    Use
    Tags
    Sumo
    Expression
    Mammalian
    Mutation
    encodes only residues 142-515 of DELE1 (represent…
    Promoter
    Available since
    Oct. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    226107-plasmid-map-sequence-id-449375

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Showing: 101 - 150 of 664 results