2,202 results
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Plasmid#82400PurposeGateway-compatible zebrafish elavl3 promoterDepositorInsertelavl3 promoter
Available SinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5E-1.7kbfabp6
Plasmid#159087Purposep5E with promoter sequence 1.7kb upstream of zebrafish fabp6 geneDepositorInsert1.7kb fabp6 promoter (fabp6 Zebrafish)
UsePromoter fragment 5' entry vector for tol2 c…Promoter1.7kb fabp6Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS U6a gRNA
Plasmid#158961PurposegRNA backbone driven by zebrafish U6a promoterDepositorInsertszf U6a promoter
gRNA backbone
UseCRISPRAvailable SinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-mRFP-; gcry1:BFP -2
Plasmid#173888PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-mRFP; gcry1: BFP
UseSynthetic BiologyAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDrVSP(C302S)-IRES2-EGFP
Plasmid#80331PurposeExpresses Danio rerio VSP (without phosphatase activity) with separated EGFP in mammalian cellDepositorInsertDaino rerio voltage-sensing phosphatase (tpte Zebrafish)
ExpressionMammalianMutationC302SPromoterCMVAvailable SinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2-nCas9n-nanos 3’UTR
Plasmid#62542Purposeexpression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafishDepositorAvailable SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR mitfa:EGFP
Plasmid#118850PurposeExpresses EGFP and zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertmitfa: EGFP (mitfa Synthetic, Zebrafish)
UseZebrafish expression, tol6Available SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tol2-krt4-GFP
Plasmid#97151Purposelabel apical epithelial cells in zebrafishDepositorInsertAcGFP
UseZebrafishAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)
Plasmid#140892PurposeExpresses modified Danio rerio VSP with enhanced phosphatase activity fused with mCherry in mammalian cells.DepositorTagsmCherryExpressionMammalianMutationchanged 223L to F in Dr-VSP (zebrafish TPTE)Available SinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM-myr-tdTomato-SV40pA-FKF
Plasmid#119867PurposeDonor cassette containing myr-tagged tdTomato and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertmyr-tagged tdTomato and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
Tags2x myristoylationExpressionBacterialAvailable SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
p5E--3mnx1
Plasmid#74632PurposeCan be used to drive expression specifically in zebrafish motor neuronsDepositorInsertmotor neuron and pancreas homeobox 1 promoter (mnx1 Zebrafish)
Use5' entry vector for multisite gateway three …Promoter-3mnx1Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry
Plasmid#191500PurposeGROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eyeDepositorInsertCaSR-EGFP-SV40
UseCreation of transgenic fish using tol2 transgenes…Promotermyo6bAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKHR8
Plasmid#74625Purposea plasmid derived from pKHR5: in addition to Venus reporter gene, it also contains cmlc2::mCherry (red heart) reporter gene at 3’ end of the mcs as pKHR7. Genbank accession number is KU144825.DepositorInsertsFRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
cmlc2::mCherry
UseCre/Lox; Zebrafish expressionPromoteralpha-crystallin and cmlc2Available SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
mhc2dab:GFP-LT
Plasmid#68996Purposefor making transgenic fishDepositorAvailable SinceNov. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST mfap4:tdTomato
Plasmid#135214PurposeRed tagged macrophagesDepositorInsertmfap4 promoter and tdTomato
UseZebrafish expressionAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGTag-eGFP-SV40
Plasmid#117813PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInserteGFP
UseSynthetic BiologyAvailable SinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2-zH2B-GFP
Plasmid#226561PurposeExpress H2B-GFP in zebrafish.DepositorAvailable SinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
ins:CFP-NTR
Plasmid#66593PurposeSpecific and conditional cell ablation of beta cells in the zebrafish pancreasDepositorInsertInsulin promotor driving CFP-nitroreductase
UseZebrafish expressionTagsCFP fused with N-terminus of nitroreductasePromoterZebrafish Insulin promoter (approximately 1.2kb)Available SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5E-dr-myl7-pro (JDW 1148)
Plasmid#229836PurposeA gateway compatible 5' entry clone containing the mysoin light chain seven (myl7/cmlc2) promoter for cardiomyocyte specific expression.DepositorInsertcmlc2/myl7
UseGateway CloningAvailable SinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGTag-TagRFP-SV40
Plasmid#117807PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
p5E LyzC
Plasmid#135200PurposeNeutrophil specific labelDepositorInsertLyz promoter
UseSynthetic BiologyPromoterLyzCAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-olig2
Plasmid#192743PurposeGateway entry vector encoding zebrafish olig2DepositorInsertolig2 (olig2 Zebrafish)
UseGateway CloningMutationS61A (rs501471300); G235S (rs505716173)PromoterNoneAvailable SinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-lyzC-RFP-miR-223
Plasmid#97148PurposemiR-223 expressionDepositorInsertmiR-223
UseZebrafishAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2KXIGdeltaIn-MCS-Avi-EGFP-rpl10a
Plasmid#58380Purposetol2 transgenesis for biotin based TRAP - Avi tagged RibosomeDepositorInsertavi-EGFP-rpl10a
TagsAvi Tag and EGFPAvailable SinceAug. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTOPO-col10a1-KI-donor
Plasmid#184874PurposeDonor template for knock-in of p2a-CreERT2 into the medaka col10a1 locus via CRISPR/Cas9-mediated homology directed repairDepositorInsertsCollagen10a1 5' homology arm
Collagen10a1 3' homology arm
p2a-CreERT2
Myosin light chain 2 promoter
eGFP
UseCRISPR and Cre/LoxAvailable SinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
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hzHDAC10
Plasmid#225542PurposeExpresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.DepositorInsertHistone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)
TagsHis6-MBP tag with cleavable TEV siteExpressionBacterialMutationMutated A24E and D94APromoterT7Available SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
zCREST3:redCas9; control_gRNA
Plasmid#199334PurposepDEL157; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous control sgRNADepositorInsertszCREST3
Cas9
control sgRNA
UseTol2 destination vectorAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5'Entry-lck-7.4kb
Plasmid#58891Purposeprovides zebrafish lck promoter (7.4 kb) for GateWay construction of Tol2 transgenesDepositorInsertzebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 7.4 kb
UseGateway 5' entry cloneMutationmutated single nucleotide (T-->C, 1874th nucle…Promoterlymphocyte-specific protein tyrosine kinase (lck)…Available SinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2+cPLA2-mKate2
Plasmid#162068PurposeUsed to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseAvian, xenopus, zebrafishTagsmKate2ExpressionMammalianPromoterCMVAvailable SinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pISceI Dusp6:D2EGFP
Plasmid#32667DepositorAvailable SinceNov. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
gfap-EGFP donor
Plasmid#65564PurposeIntron targeting-mediated EGFP knockin at the zebrafish gfap locusDepositorAvailable SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tol2-UAS-Dendra2
Plasmid#97145PurposeFor Gal4/UAS expression systemDepositorInsertDendra2
UseZebrafishAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tg (mpx:mCherry-2A-Rac2D57N)
Plasmid#58934Purposeexpresses dominant negative rac2 tagged with mcherry in neutrophilsDepositorInsertmcherry-2a-Rac2D57N (rac2 Zebrafish)
Tagsmcherry2aMutationchanged Aspartate 57 to AsparaginePromotermpxAvailable SinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj13-EGFP
Plasmid#164940PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminusDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsEGFPMutationStop codon deletedPromoteractinb (actb2) promoterAvailable SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBK-CMV-mpx
Plasmid#191826Purposempx probe generation for in situDepositorAvailable SinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDest-1.7kbfabp6-eGFP-pA-CrymCherry
Plasmid#159088PurposeLR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA insertsDepositorInsertsUseFluorescent reporterPromoter1.7kb fabp6Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
PTRF/Cavin1_R (OZ514)
Plasmid#27189DepositorInsertZinc finger array targeting PTRF/Cavin1 (cavin1b Zebrafish)
UseZebrafish targetingAvailable SinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
p5'Entry-lck-5.5kb
Plasmid#58890Purposeprovides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenesDepositorInsertzebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
UseGateway 5' entry cloneMutationmutated single nucleotide (T-->C, 13th nucleot…Promoterlymphocyte-specific protein tyrosine kinase (lck)…Available SinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGTag-gal4-VP16-B-actin
Plasmid#117817PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertgal4/VP16
UseSynthetic BiologyAvailable SinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR mitfa:BRAF
Plasmid#118846PurposeExpresses human BRAF V600E mutant and zebrafish mitfa specifically in zebrafish melanocytesDepositorAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK011 p5E exorh
Plasmid#195949PurposeGateway p5E vectorDepositorInsertexorh regulatory elements
UseOtherAvailable SinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5E-dusp6
Plasmid#82584Purpose5' entry vector containing FGF-responsive regulatory element of the zebrafish dusp6 promoter for FGF-responding cellsDepositorInsertFGF-responsive element of the dusp6 promoter
PromoterNoneAvailable SinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestGFP_RNAseH1
Plasmid#65784Purposeexpresses GFP-tagged RNAseH1 in mammalian cellsDepositorAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
I-SceI pB 5UAS::ZfSyGCaMP2
Plasmid#26125DepositorAvailable SinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
M28 Nac>GFP
Plasmid#17178DepositorInsertnacre promoter (mitfa Zebrafish)
TagsGFPAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
p5E-acta2
Plasmid#78686Purposeacta2 promoter for Gateway cloningDepositorAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP1-sox17 promoter
Plasmid#31400DepositorAvailable SinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-mRFP-; gcry1:BFP -0
Plasmid#173886PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-mRFP; gcry1: BFP
UseSynthetic BiologyAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Cre-cmlc2-mRFP
Plasmid#117793PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertCre
UseSynthetic BiologyAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only