165,775 results

1. AbVec2.0-IGHG1

   Plasmid

   #80795

   Purpose

   Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

   Depositor

   Hedda Wardemann

   Article

   Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   hCMV IE1 gene promoter

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABE-hygro-hTERT

   Plasmid

   #1773

   Purpose

   Retroviral expression of hTERT. Used to create immortalized cell lines.

   Depositor

   Bob Weinberg

   Article

   Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

   Insert

   hTERT (TERT Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. ppyCAG_RNaseH1_WT

   Plasmid

   #111906

   Purpose

   Express RNASEH1 in mammalian cell.

   Depositor

   Xiang-Dong Fu

   Article

   Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

   Insert

   RNASEH1 (RNASEH1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   July 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. LT3GEPIR

   Plasmid

   #111177

   Purpose

   Tet-ON miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. nlsSalicS1

   Plasmid

   #232190

   Purpose

   Novel FRET-based biosensor for salicylic acid

   Depositor

   Alexander Jones

   Article

   Tang et al Science. 2025 Oct 9;390(6769):188-194. d

   Insert

   nlsSalicS1
   

   Tags

   His-tag

   Expression

   Plant

   Promoter

   p16

   Available Since

   Oct. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. ppyCAG_RNaseH1_D210N

   Plasmid

   #111904

   Purpose

   Express D210N mutated RNASEH1 which abolishes the catalytic activity of RNASEH1

   Depositor

   Xiang-Dong Fu

   Article

   Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

   Insert

   RNASEH1 (RNASEH1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed D 210 to N

   Available Since

   July 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. T7-VEE-GFP

   Plasmid

   #58977

   Purpose

   expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Insert

   EGFP
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Expression

   Mammalian

   Promoter

   26S subgenomic promoter

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVUTHshGATA1-tTR-KRAB

   Plasmid

   #11650

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for shGATA1 (hUbiquitin promoter) - 3rd generation

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   hUbiquitin C, GFP, tTR-KRAB, shRNA against GATA1, Tet-on (GATA1 Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

   Plasmid

   #108871

   Purpose

   Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

   Depositor

   Alex Schier

   Article

   Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

   Inserts

   5xU6:sgRNA
   Cas9-t2A-GFP
   

   Use

   CRISPR

   Promoter

   U6 and hsp70

   Available Since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-Tau(WT)

    Plasmid

    #187023

    Purpose

    Expresses EGFP tagged human 2N4R tau (WT) in mammalian cells with CMV promoter

    Depositor

    George Bloom

    Article

    Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.

    Insert

    MAPT (MAPT Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-TDP-43 (Q331K)

    Plasmid

    #235488

    Purpose

    Expression of human TDP-43-EGFP with human ALS-causing Q331K mutation

    Depositor

    Maxime Rousseaux

    Article

    Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

    Insert

    TARDBP (TARDBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q331K

    Available Since

    April 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCMV-PuroR-2A-MLH1-SB

    Plasmid

    #228871

    Purpose

    Plasmid for MLH1-SB overexpression

    Depositor

    Sangsu Bae

    Article

    Park et al Cell. 2025 Aug 1:S0092-8674(25)00799-8.

    Insert

    PuroR-2A-MLH1-SB
    

    Use

    CRISPR

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. PLKO.1-Scrambled

    Plasmid

    #136035

    Purpose

    Scrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    None (Scrambled)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. V5-TurboID-NES_pCDNA3

    Plasmid

    #107169

    Purpose

    expresses V5-tagged TurboID in the mammalian cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. wtTDP43tdTOMATOHA

    Plasmid

    #28205

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Tags

    HA tag and tdtomato

    Expression

    Mammalian

    Available Since

    June 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFGET19_Ulp1

    Plasmid

    #64697

    Purpose

    H6-Ulp1 from pHYRS52 into pET-28b(+)

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Insert

    ULP1 (ULP1 Budding Yeast, Saccharomyces cerevisiae)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Ulp1(403-621)

    Promoter

    T7

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. circRNA-synIRES-R25-mNeonGreen

    Plasmid

    #188115

    Purpose

    Template plasmid to transcribe circular RNA encoding mNeonGreen

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pH6-MBP-TEV-BirA

    Plasmid

    #179694

    Purpose

    Expresses His6 tagged MBP-BirA fusion protein

    Depositor

    Mark Arbing

    Insert

    BirA
    

    Tags

    His6-MBP

    Expression

    Bacterial

    Available Since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-PE6b

    Plasmid

    #207852

    Purpose

    Mammalian expression of PE6b prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6b
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLJM1-Tmem192-mRFP-3xHA

    Plasmid

    #134631

    Purpose

    bait for affinity-isolation of lysosomes

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    3xHA, TEV, and mRFP

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pT4-CMV-GFP

    Plasmid

    #117046

    Purpose

    Optimized Sleeping Beauty Transposon Vector for GFP expression by a CMV promoter

    Depositor

    Wolfgang Uckert

    Insert

    GFP
    

    Use

    Transposon

    Promoter

    CMV

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pJT260 Lenti pEF-dCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast

    Plasmid

    #187336

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. M51 Super 8x FOPFlash (TOPFlash mutant)

    Plasmid

    #12457

    Purpose

    Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

    Depositor

    Randall Moon

    Article

    Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

    Insert

    mutant TCF/LEF binding sites (Ctnnb1 )
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    mutant TCF/LEF binding sites

    Available Since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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24. pMRX-IP-GFP-LC3-RFP-LC3ΔG

    Plasmid

    #84572

    Purpose

    Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Available Since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC

    Plasmid

    #221357

    Purpose

    Mammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC; to be used with Anti-DYKDDDDK [IPI-4E11/M1] light chain (Plasmid 221358) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC
    

    Use

    Affinity Reagent/ Antibody

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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26. pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa

    Plasmid

    #221358

    Purpose

    Mammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa; to be used with Anti-DYKDDDDK [IPI-4E11/M1] heavy chain (Plasmid 221357) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa
    

    Use

    Affinity Reagent/ Antibody

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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27. HyPer7.2DAAO-mito

    Plasmid

    #168304

    Purpose

    Chemogenetic fluorescent H2O2 reporter targeted to the mitochondria

    Depositor

    Thomas Michel

    Article

    Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

    Insert

    HyPer7.2DAAO-mito
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. dCAS9-VP64_GFP

    Plasmid

    #61422

    Purpose

    Expresses dCAS9-VP64 activator with 2A GFP

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Concentrated Lentiviral Prep

    Insert

    dCAS9(D10A,H840A)-VP64_2A_GFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    EF1A

    Available Since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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29. AbVec1.1-IGKC

    Plasmid

    #80796

    Purpose

    Expression of immunoglobulin light chains in mammalian cells, human kappa constant region

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PB_tre_Cas9

    Plasmid

    #126029

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible Cas9

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    humanized S. pyrogenes Cas9
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Expression

    Mammalian

    Promoter

    EF1 alpha and TRE

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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