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178,651 results

Showing: 931 - 960 of 178651 results

  4. CROP-seq-opti

    Plasmid
    #106280
    Purpose
    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.
    Depositor
    Jay Shendure
    Publication
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    106280-plasmid-map-sequence-id-512176

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  9. pCMV14-3X-Flag-SARS-CoV-2 S

    Plasmid
    #145780
    Purpose
    Over expression of SARS-CoV-2 S (spike) in mammalian cell line
    Depositor
    Zhaohui Qian
    Publication
    Ou et al Nat Commun. 2020 Mar 27;11(1):1620. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S (S SARS CoV 2)
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Codon optimized and C-terminal 19 amino acids del…
    Promoter
    CMV
    Available since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    145780-plasmid-map-sequence-id-354441

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  21. pcDNA3-GFP-LC3-RFP-LC3ΔG

    Plasmid
    #168997
    Purpose
    Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux
    Depositor
    Noboru Mizushima
    Publication
    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    Tags
    EGFP and mRFP1
    Expression
    Mammalian
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    168997-plasmid-map-sequence-id-414009

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  26. pAAV.CMV.PI.EGFP.WPRE.bGH

    Plasmid
    #105530
    Purpose
    AAV expression of EGFP from CMV promoter
    Depositor
    James M. Wilson
    Has service
    AAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.eB, AAV Retrograde, and AAV rh10
    Insert
    EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105530-plasmid-map-sequence-id-454420

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  28. pCMV-lyso-pHluorin

    Plasmid
    #70113
    Purpose
    Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification
    Depositor
    Christian Rosenmund, Benjamin Rost
    Publication
    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    lyso-pHluorin (Cd63 Aequorea victoria, Mouse)
    Tags
    HA-Tag and pHluorin
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70113-plasmid-map-sequence-id-323570

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Showing: 931 - 960 of 178651 results