163,570 results

1. pEJS2706-CMV-Nme2-ABE8e-i8-L10-E932R

   Plasmid

   #228727

   Purpose

   Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2 nCas9 (D16A)-i8-L10-E932R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A E932R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEJS2707-CMV-Nme2-ABE8e-i8-L10-D56R

   Plasmid

   #228728

   Purpose

   Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2 nCas9 (D16A)-i8-L10-D56R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A D56R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEJS2709-CMV-Nme2Smu-ABE8e-i1-L10-D56R

   Plasmid

   #228730

   Purpose

   Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2Smu nCas9 (D16A)-i1-L10-D56R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A D56R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEJS2712-CMV-eNme2-C-ABE8e-i1-L10

   Plasmid

   #228733

   Purpose

   Expresses domain inlaid eNme2-C nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid eNme-C nCas9 (D16A)-i1-L10
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Stx5S-mCherry

   Plasmid

   #165087

   Purpose

   Expresses Stx5S-mCherry in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   Stx5 (Short isoform) (STX5 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Lacks amino acids 1-54, enocdes short isoform of …

   Promoter

   CMV

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAB107a

   Plasmid

   #79218

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ-opt (OR1); Ppro1

   Available Since

   May 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NELC492A-mcherry-Tnos

   Plasmid

   #211845

   Purpose

   Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase, where the ligase is catalytically inactive.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Inserts

   LRR-NEL
   GAL4-VP16-GR
   

   Use

   Synthetic Biology

   Tags

   HA, Myc, and mCherry

   Expression

   Plant

   Mutation

   C492A

   Promoter

   6xUAS-35Smini and pUBQ10

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TFORF1684

   Plasmid

   #143965

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   HNF1B (HNF1B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. H_NES 41kDa

   Plasmid

   #201346

   Purpose

   Nucleocytoplasmic transport probe

   Depositor

   Pere Roca-Cusachs

   Article

   Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

   Insert

   HIV_NES-EGFP-2PrA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. UAS-Syt::7xHalo7

    Plasmid

    #87650

    Purpose

    For creating drosophila transgenics expressing UAS-Syt::7xHalo7

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    Syt::7xHalo7
    

    Use

    Tags

    Expression

    Insect

    Mutation

    S265G in Syt (please see depositor's commen…

    Promoter

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLXI_TRC401 SMARCB1 var 2

    Plasmid

    #111182

    Purpose

    Inducible vector (untagged) with SMARCB1 variant 2

    Depositor

    William Hahn

    Article

    Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

    Insert

    BAF47 (SMARCB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBlueBac human APC2

    Plasmid

    #11425

    Depositor

    Marc Kirschner

    Article

    Yu et al Science. 1998 Feb 20. 279(5354):1219-22.

    Insert

    APC2 (ANAPC2 Human)
    

    Use

    Baculoviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pDONR221_SLC8A1

    Plasmid

    #132162

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC8A1 (SLC8A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. mCherry-Moesin-N-14

    Plasmid

    #55104

    Purpose

    Localization: Cytoskeleton/Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Moesin (MSN Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pMC-1-ZmPLTP-6

    Plasmid

    #197713

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    ZmPLTP promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPGK-T7/2-CD44v9 (fl)

    Plasmid

    #137818

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v9 (fl) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    full length and mutation H48L

    Promoter

    PGK

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pF3BGX-T2A-lexAp65

    Plasmid

    #138393

    Purpose

    RMCE vector for generating C-terminal T2A-lexA-p65 fusion

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    T2A-lexAp65
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NAX_CAGGS_CAS9_mcherry

    Plasmid

    #167862

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. betaTrCP mVenus reporter

    Plasmid

    #192458

    Purpose

    Fluorescent reporter for beta-TrCP activity in human cells

    Depositor

    Steven Cappell

    Article

    Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

    Insert

    CDC25B (CDC25B Human)
    

    Use

    Lentiviral

    Tags

    SV40 NLS and mVenus

    Expression

    Mammalian

    Mutation

    aa198-338

    Promoter

    EF1alpha

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. betaTrCP mVenus mutant reporter

    Plasmid

    #192459

    Purpose

    Fluorescent reporter for beta-TrCP with a non-degradable mutation. Used as a control.

    Depositor

    Steven Cappell

    Article

    Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

    Insert

    CdC25B
    

    Use

    Lentiviral

    Tags

    SV40 NLS and mVenus

    Expression

    Mammalian

    Mutation

    aa198-338 of CDC25B. betaTrCP degron sequence (aa…

    Promoter

    EF1alpha

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 6PBG

    Plasmid

    #162263

    Purpose

    Bacterial Expression

    Depositor

    Cheryl Arrowsmith

    Insert

    COPA (COPA Human)
    

    Use

    Baculovirus expresssion

    Tags

    6xHis-TEV

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available Since

    Dec. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pNP1 eforRed trigger

    Plasmid

    #107357

    Purpose

    Trigger sequence for pNP1 eforRed toehold switch

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold trigger 1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBACgus_Sumostar/LRRK2 1867-2186

    Plasmid

    #40386

    Depositor

    Michael J Fox Foundation MJFF

    Inserts

    SUMO
    LRRK2 (LRRK2 Human)
    

    Use

    Baculovirus

    Tags

    6xHis and HisTEV

    Expression

    Mutation

    WT aa1867-2186

    Promoter

    polH

    Available Since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. mCherry-Ezrin-C-14

    Plasmid

    #55042

    Purpose

    Localization: Focal Adhesions/Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    NM_003379.4 (EZR Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

25. Bet1-mCherry

    Plasmid

    #209848

    Purpose

    Expresses Bet1-mCherry in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    Bet1 (BET1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mCherry-TMCC1

    Plasmid

    #120932

    Purpose

    Expresses human TMCC1 in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

    Insert

    Trans membrane and coiled coil domain containing protein 1 (TMCC1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAB064d

    Plasmid

    #79197

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Plac62 luxAB; Ppro1 434cI-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac62; Ppro1

    Available Since

    Dec. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPGK-T7/2-CD44s (-cyt)

    Plasmid

    #137815

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44s (-cyt) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region

    Promoter

    PGK

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSMOX-mNG::LacI-V3

    Plasmid

    #210224

    Purpose

    Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    mNeongreen Lac repressor fusion protein
    

    Use

    Trypanosoma brucei expression

    Tags

    Expression

    Mutation

    Promoter

    Pol II readthrough transcription after integration

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SB52

    Plasmid

    #21487

    Depositor

    Shoumo Bhattacharya

    Article

    Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

    Insert

    Cited2 (CITED2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 23, 2009

    Availability

    Academic Institutions and Nonprofits only