Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

161,273 results

Showing: 931 - 960 of 161273 results

  4. PPDK

    Plasmid
    #64037
    Purpose
    Expression of Pyruvate phosphate dikinase (PPDK) in E. coli
    Depositor
    Vern Schramm
    Article
    Sturm et al Anal Chem. 2009 Apr 15;81(8):2847-53. do
    Insert
    pyruvate phosphate dikinase
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pcDNA3-ER-AKAR4

    Plasmid
    #64733
    Purpose
    Endoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.
    Depositor
    Jin Zhang
    Article
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    AKAR4
    Use
    Tags
    Cerulean, N-terminal 27 amino acids from CYP450, …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64733-plasmid-map-sequence-id-109352

    Enlarge

  8. PANK3

    Plasmid
    #32872
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PANK3 (PANK3 Human)
    Use
    Tags
    His tag (with Thrombin cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 10-370 only
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. p667-UBC-SCYL1-V5-miniTurbo_IDG-K

    Plasmid
    #189911
    Purpose
    Gateway destination clone of SCYL1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    SCYL1 (SCYL1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    189911-plasmid-map-sequence-id-374157

    Enlarge

  13. TFORF1216

    Plasmid
    #144090
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NHLH1 (NHLH1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    144090-plasmid-map-sequence-id-391502

    Enlarge

  20. tdTomato-Rab7a-7

    Plasmid
    #58127
    Purpose
    Localization: Ras GTPase, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    Rab7a (RAB7A Human)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  21. pAcBac1-Ma-Acridone-RS1 (A7)

    Plasmid
    #197566
    Purpose
    Expression of Methanomethylophilus alvus (Ma) Acridone RS1 (A7) tRNA synthetase/Pyl-tRNA pair for the encoding of Acridone at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    M. alvus PylRS - Acd RS1 (A7)
    M. alvus Pyl-tRNA (6) (4x copies)
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    N166A, V168G, W239C
    Promoter
    CMV and U6/H1
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197566-plasmid-map-sequence-id-388228

    Enlarge

  27. pCK341

    Plasmid
    #192640
    Purpose
    Expresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmR
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102
    Inserts
    Sp.pCas9-dSpRY
    BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    SoxS has R93A and S101A mutations and dSpRY has s…
    Promoter
    BBa_J23107 and Sp.pCas9
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    192640-plasmid-map-sequence-id-381518

    Enlarge

  29. pFGL822

    Plasmid
    #58225
    Purpose
    For fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.
    Depositor
    Naweed Naqvi
    Article
    Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,
    Insert
    phosphinothricin acetyl transferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans trpC promoter
    Available since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    58225-plasmid-map-sequence-id-115839

    Enlarge

Showing: 931 - 960 of 161273 results