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We narrowed to 4,645 results for: PEP

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Showing: 1601 - 1650 of 4645 results

  4. sfGFP-ER-5

    Plasmid
    #56483
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 485, Emission: 507
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Tags
    sfGFP
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available Since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. pcDNA3-sACE2v2(732)-IgG1

    Plasmid
    #154105
    Purpose
    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (IgG1-Fc fusion)
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Fc region of human IgG1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease and neck domains …
    Promoter
    CMV
    Available Since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154105-plasmid-map-sequence-id-304581

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  8. pelB-MBP-BB0238-ss

    Plasmid
    #137046
    Purpose
    E. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66635.2 (BB_0238 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature BB0238: lacks the BB0238 signal peptide
    Promoter
    T7lac
    Available Since
    April 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    137046-plasmid-map-sequence-id-292071

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  9. S10A mutant of H3S10ph biosensor

    Plasmid
    #120810
    Purpose
    FRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capability
    Depositor
    Yingxiao Wang
    Article
    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p
    Insert
    Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP
    Expression
    Mammalian
    Mutation
    Serine 10 is mutated to Alanine, and Threonine 3,…
    Promoter
    CMV
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120810-plasmid-map-sequence-id-235276

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  12. pFP.R267

    Plasmid
    #138179
    Purpose
    Expresses the split mCherry reporter interrupted by the gp41-1 intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(SGY).gp41-1.(SSS)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138179-plasmid-map-sequence-id-273462

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  13. YWHAB

    Plasmid
    #39128
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    YWHAB (YWHAB Human)
    Tags
    Tev His6
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. pFP.E227

    Plasmid
    #138249
    Purpose
    Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    mCherry
    Terminator
    mTagBFP
    Terminator
    gfp
    Terminator
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P17_up1691, P20_992, and iP16_3622
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138249-plasmid-map-sequence-id-273529

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  17. p-spTorA-GFP-H6C

    Plasmid
    #168517
    Purpose
    GFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension.
    Depositor
    Siegfried Musser
    Article
    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi
    Insert
    spTorA-GFP-H6C
    Tags
    6xHisC tag and spTorA (signal peptide of E. coli …
    Expression
    Bacterial
    Mutation
    The mut3 GFP variant with 5 additional mutations …
    Promoter
    araBAD
    Available Since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    168517-plasmid-map-sequence-id-346120

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  22. pGBW-m4092694

    Plasmid
    #153907
    Purpose
    Bacterial expression plasmid for fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | t7 consensus from NEB, G is +1
    Available Since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153907-plasmid-map-sequence-id-307331

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  25. pSPYngK+-hp

    Plasmid
    #48121
    Purpose
    Shuttle vector E. coli/B.meg.; carrying the codon optimized sequence of the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):
    Insert
    codon optimized signal peptide of YngK
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Oct. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    48121-plasmid-map-sequence-id-69947

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  34. MSP2-bio

    Plasmid
    #47710
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP2 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP2
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47710-plasmid-map-sequence-id-68424

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  35. pET His6 MBP TEV HDAC8_374

    Plasmid
    #122174
    Purpose
    Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag
    Depositor
    David Christianson
    Article
    Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22
    Insert
    His6 MBP TEV HDAC8_374 (HDAC8 Human)
    Tags
    hexahistidine tag and tobacco etch virus protease…
    Expression
    Bacterial
    Mutation
    Residues Met1–Pro7 and H375-V377 have been delete…
    Promoter
    T7
    Available Since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    122174-plasmid-map-sequence-id-237469

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  36. GIPR-DuET

    Plasmid
    #213252
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GIPR (GIPR Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213252-plasmid-map-sequence-id-430802

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  38. pcDNA3_erGAP2

    Plasmid
    #78120
    Purpose
    Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum
    Depositor
    Teresa Alonso, Javier García-Sancho
    Insert
    erGAP2
    Tags
    KDEL and calreticulin signal peptide
    Expression
    Mammalian
    Mutation
    D24N/D119A in aequorin moiety
    Promoter
    CMV
    Available Since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    78120-plasmid-map-sequence-id-153381

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  40. Flag-mCherry-Binder

    Plasmid
    #179139
    Purpose
    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).
    Depositor
    Klaus Hahn
    Article
    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.
    Insert
    SspB
    Tags
    FLAG and mCherry
    Expression
    Mammalian
    Mutation
    Y11K/A15E, residue numbering from pdb: 1ou9
    Available Since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179139-plasmid-map-sequence-id-353408

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  41. pCAP03-ermE*p

    Plasmid
    #120234
    Purpose
    Carries promoter cassette containing ermE*p promoter and ampicillin resistance gene that can be amplified with homology sequence for refactoring.
    Depositor
    Bradley Moore
    Article
    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-
    Insert
    promoter cassette containing ermE*p promoter and ampicillin resistance gene
    Use
    Synthetic Biology
    Mutation
    bla (amp resistance gene)
    Available Since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    120234-plasmid-map-sequence-id-233748

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  42. CYP11A1 (3N9Y)

    Plasmid
    #28119
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CYP11A1:HPC09N-C05:C214491 (CYP11A1 Human)
    Expression
    Bacterial
    Available Since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  45. TFORF0235

    Plasmid
    #142488
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MYCN (MYCN Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    142488-plasmid-map-sequence-id-353633

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  47. 2xMARS-nGFP

    Plasmid
    #205235
    Purpose
    Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells
    Depositor
    Jeremy Baskin
    Article
    Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816
    Insert
    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    Tags
    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available Since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205235-plasmid-map-sequence-id-404746

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Showing: 1601 - 1650 of 4645 results