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Plasmid#206213PurposePiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplificationDepositorInserttetracycline-responsive transactivator
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
vYM245_PB-Blast-pEF1a-H2B-mCh-D3bCI
Plasmid#202596Purposeplasmid for expressing the DNMT3bCI (catalytic inactive)DepositorInsertH2B-mCherry-T2A-rTetR-DNMT3bCI (Dnmt3b Synthetic, Mouse)
UseSynthetic BiologyTagsrTetRExpressionMammalianMutationP656V, C657DPromoterAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
vYM010_phiC31-nTRE-pEF1s(high)-H2b-Citrine
Plasmid#197678Purpose"pEF1s(high)" reporter plasmidDepositorInsertpEF1s-H2B-Citrine
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
vYM011_phiC31-nTRE-pEF1s(low)-H2b-Citrine
Plasmid#197679Purpose"pEF1s(low)" reporter plasmidDepositorInsertpEF1s-H2B-Citrine
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
vYM141_phiC31_nTRE-pEF1s(orig)(CC793)-H2B-citrine
Plasmid#197684Purpose"pEF1s(orig)" reporter plasmid, with CpG793 mutated to CCDepositorInsertpEF1s-H2B-Citrine
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgT-REX17_EF1a-Puro-T2A-BFP
Plasmid#195501PurposeLentiviral BFP expression vector bearing a sgRNA targeting the promoter of human T-REX17DepositorInsertsgRNA
UseCRISPR and LentiviralTagsBFP, Puro, and T2AExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-mGFP-MutCREB(S133A)
Plasmid#194642PurposeExpresses the fusion of monomeric-EGFP-tagged mutant CREB(S133A) in a Cre-dependent mannerDepositorInsertCREB (Creb1 Rat)
UseAAVTagsmEGFPExpressionMutationS133APromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB EF1a CCR6-eLOV-TEVcs-Gal4
Plasmid#173119PurposeExpresses CCR6 eLOV receiver construct in DIPGDepositorInsertCCR6-eLOV-TEVcs-Gal4
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB EF1a CCR6-hLOV-TEVcs-Gal4
Plasmid#173120PurposeExpresses CCR6 hLOV receiver construct in DIPGDepositorInsertCCR6-hLOV-TEVcs-Gal4
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX552-mScn8a-3xV5-EF1-smFP-flag
Plasmid#182562PurposeFor mouse Nav1.6 knockin with 3xV5 at the C-terminalDepositorInsertsmouse Scn8a gRNA and 3xV5 donor DNA
smFP-flag
UseAAV and CRISPRTagsExpressionMutationPromoterEF1 and U6Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-Ef1A-Myc-POLB(PAMmut)
Plasmid#176150PurposeN-terminus Myc-tagged POLB containing a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassetteDepositorInsertPolB (POLB Human)
UseLentiviralTagsMYCExpressionMammalianMutationMutation in the PAM site used by POLB gRNA1PromoterEF1AAvailable sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-squirrel-monkey-full-length-CHMP3-HA
Plasmid#154172Purposeexpresses squirrel monkey CHMP3 in mammalian cellsDepositorInsertCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-3)
Plasmid#159664PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2)
Plasmid#159663PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1)
Plasmid#159662PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-1)
Plasmid#159656PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM457 EF1alpha EBFP2-P2A-BlastR (TUPV8)
Plasmid#139237PurposemMoClo TUPV8, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCSDepositorInsertEBFP2-P2A-Blasticidin S Deaminase
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM456 EF1alpha EBFP2-P2A-BlastR (TUPV7)
Plasmid#139236PurposemMoClo TUPV7, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCSDepositorInsertEBFP2-P2A-Blasticidin S Deaminase
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM454 EF1alpha EBFP2-P2A-BlastR (TUPV5)
Plasmid#139234PurposemMoClo TUPV5, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCSDepositorInsertEBFP2-P2A-Blasticidin S Deaminase
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM453 EF1alpha EBFP2-P2A-BlastR (TUPV4)
Plasmid#139233PurposemMoClo TUPV4, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCSDepositorInsertEBFP2-P2A-Blasticidin S Deaminase
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM452 EF1alpha EBFP2-P2A-BlastR (TUPV3)
Plasmid#139232PurposemMoClo TUPV3, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCSDepositorInsertEBFP2-P2A-Blasticidin S Deaminase
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM451 EF1alpha EBFP2-P2A-BlastR (TUPV2)
Plasmid#139231PurposemMoClo TUPV2, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCSDepositorInsertEBFP2-P2A-Blasticidin S Deaminase
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM589 EF1α intN-ZF10-NLS-HA in TUPV2
Plasmid#161565PurposeConstitutive expression of intN-ZF10-NLS-HA under the EF1α promoterDepositorInsertintN-ZF10-NLS-HA
UseSynthetic BiologyTagsHAExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM516 EF1α intC-ZF1-NLS-HA in TUPV2
Plasmid#161551PurposeConstitutive expression of intC-ZF1-NLS-HA under the EF1α promoterDepositorInsertintC-ZF1-NLS-HA
UseSynthetic BiologyTagsHAExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM522 EF1α intC-ZF10-NLS-HA in TUPV2
Plasmid#161552PurposeConstitutive expression of intC-ZF10-NLS-HA under the EF1α promoterDepositorInsertintC-ZF10-NLS-HA
UseSynthetic BiologyTagsHAExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM503 EF1α 3xFLAG-NLS-ZF1 in TUPV2
Plasmid#161534PurposeConstitutive expression of 3xFLAG-NLS-ZF1 under the EF1α promoterDepositorInsert3xFLAG-NLS-ZF1
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-UBC-irfp luc-EF1 Hygro
Plasmid#129438Purposeexpress irfp-luc chimeraDepositorInsertirfp-luc
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-3
Plasmid#128764PurposeExpresses DIS3L2 gRNA for CRISPRiDepositorInsertDIS3L2 sgRNA #3 (DIS3L2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-2
Plasmid#128763PurposeExpresses DIS3L2 gRNA for CRISPRiDepositorInsertDIS3L2 sgRNA #2 (DIS3L2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-1
Plasmid#128762PurposeExpresses DIS3L2 gRNA for CRISPRiDepositorInsertDIS3L2 sgRNA #1 (DIS3L2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPHAGE-EF1α-Luc-C20orf24-3’UTR-VAR
Plasmid#128507PurposeLentiviral constitutive expression of Firefly luciferase under control of 3'UTR of human C20orf24 with variant from COX deficiency patient.DepositorInsertFirefly luciferase
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.PARN-2
Plasmid#128760PurposeExpresses PARN gRNA for CRISPRiDepositorInsertPARN sgRNA #2 (PARN Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-3
Plasmid#128758PurposeExpresses PAPD4 gRNA for CRISPRiDepositorInsertPAPD4 sgRNA #3 (TENT2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-1
Plasmid#128756PurposeExpresses PAPD4 gRNA for CRISPRiDepositorInsertPAPD4 sgRNA #1 (TENT2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2
Plasmid#128757PurposeExpresses PAPD4 gRNA for CRISPRiDepositorInsertPAPD4 sgRNA #1 (TENT2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pA
Plasmid#121803PurposepMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest.DepositorInsertpolyA + EF1a::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Nkx2-5
Plasmid#117137PurposeExpression vector encoding BFP-BSD-Nkx2-5DepositorInsertNkx2-5 CDS (Nkx2-5 Mouse)
UseLentiviralTagsExpressionMammalianMutationmodified BFPPromoterhuman U6 promoter, human EF1a promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP
Plasmid#113155PurposeAn AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporterDepositorInsertTwo gRNAs for rat TH
UseAAV and CRISPRTagsExpressionMammalianMutationPromotermU6 and hU6Available sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP
Plasmid#113157PurposeAn AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporterDepositorInsertTwo gRNAs for rat MANF
UseAAV and CRISPRTagsExpressionMammalianMutationPromotermU6 and hU6Available sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-MCS2-EF1-Puro-RGS7-E383K
Plasmid#113731PurposeExpress mutant RGS7- E383K- in mamalian cellsDepositorInsertRGS7 (RGS7 Human)
UseLentiviralTagsFlagExpressionMammalianMutationE383KPromoterCMVAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-MCS2-EF1-Puro-RGS7-R44C
Plasmid#113729PurposeExpress mutant RGS7- R44C- in mamalian cellsDepositorInsertRGS7 (RGS7 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-Tmeff2-2xMyc-EF1-copGFP
Plasmid#99730Purposeoverexpression of Tmeff2 and mCherryDepositorInsertTmeff2 (Tmeff2 Mouse)
UseTags2xMycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-Tmeff2-3xFlag-EF1-nlsCopGFP
Plasmid#99729Purposeoverexpression of Tmeff2 and CopGFPDepositorInsertTmeff2 (Tmeff2 Mouse)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]
Plasmid#227246PurposeAll-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cellDepositorInsertsTet-On 3G transactivator protein
Enhanced green fluorescent protein
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1α and pTRE3GAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-Gad65-WPRE-HGHpA
Plasmid#184631PurposeExpresses Gad65, driven by the Ef1a promoter, in a Cre-dependent fashionDepositorInsertGad65 (Gad2 Mouse)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-hChR2(H134R)-P2A-EYFP
Plasmid#139283PurposeExpress hChR2(H134R)-P2A-EYFP in a Cre-dependent mannerDepositorInserthChR2(H134R)-P2A-EYFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA
Plasmid#184632PurposeExpresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashionDepositorInsertGad67 (Gad1 Mouse)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-mCherry
Plasmid#188766PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP
Plasmid#188778PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2711-pHAGE2-Ef1aL-TAZ4SA-UBC-tagBFP-loxP
Plasmid#216480Purposelentiviral dual expression of TAZ4SA and tagBFP with loxP site for Cre excisionDepositorInsertsUseCre/Lox and LentiviralTagsExpressionMutationS66A, S89A, S117A, S311APromoterEF1aL and UBCAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only