4,146 results

1. pCAG-EGFP

   Plasmid

   #89684

   Purpose

   pCAG promoter driving EGFP

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. psfaRb-Tn5

   Plasmid

   #183638

   Purpose

   Expresses anti-rabbit nanobody fused with Tn5

   Depositor

   Goncalo Castelo-Branco

   Article

   Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

   Insert

   anti rabbit secondary nanobody fused to Tn5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-TetO-hNGN2-eGFP-Puro

   Plasmid

   #79823

   Purpose

   Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.

   Depositor

   Kristen Brennand

   Article

   Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

   Insert

   hNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TetO

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. LRG2.1

   Plasmid

   #108098

   Purpose

   Lentiviral expression plasmid of sgRNA with GFP

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter for sgRNA expression and EFS promoter…

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVax1-hVEGF165

   Plasmid

   #74466

   Purpose

   expression of hVEGF165 in mammalian cells

   Depositor

   Loree Heller

   Article

   Marshall WG et al Gene Ther. 2010 Mar;17(3):419-23. doi: 1

   Insert

   vascular endothelial growth factor 165 (VEGFA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRE-mCherry

   Plasmid

   #92202

   Purpose

   Fluorescent protein reporter construct

   Depositor

   Alice Ting

   Article

   Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   June 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXPR_011

   Plasmid

   #59702

   Purpose

   This lentiviral vector can be used to assay Cas9 activity.

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

   Insert

   EGFP sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-PE6b

   Plasmid

   #207852

   Purpose

   Mammalian expression of PE6b prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6b
   

   Use

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. LZRS-DNMT1

   Plasmid

   #24952

   Depositor

   Paul Khavari

   Article

   Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

   Insert

   DNMT1 (DNMT1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag-SENP1

    Plasmid

    #17357

    Depositor

    Edward Yeh

    Article

    Cheng et al Cell. 2007 Nov 2. 131(3):584-95.

    Insert

    SENP1 (SENP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. CD63-pEGFP C2

    Plasmid

    #62964

    Purpose

    full length human CD63 cloned into pEGFP C2

    Depositor

    Paul Luzio

    Insert

    CD63 molecule (CD63 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CD63 has 1 silent mutation compared to the coding…

    Promoter

    CMV

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRGEB32

    Plasmid

    #63142

    Purpose

    Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.

    Depositor

    Yinong Yang

    Article

    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Rice snoRNA U3 promoter for gRNA/PTG expression a…

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPRia-v2

    Plasmid

    #84832

    Purpose

    CRISPRi/a V2 library parental plasmid

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Inserts

    sgRNA
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT3TS-Tol2

    Plasmid

    #31831

    Depositor

    Stephen Ekker

    Article

    Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

    Insert

    Tol2 Transposase
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Lac

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX304

    Plasmid

    #25890

    Purpose

    3rd generation lentiviral vector

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-mCherry-Mango II x 24

    Plasmid

    #127587

    Purpose

    Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter

    Depositor

    David Rueda

    Article

    Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi:

    Insert

    mCherry-Mango II x24
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE Tet ON

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSIJ8

    Plasmid

    #68122

    Purpose

    Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

    Depositor

    Alex Nielsen

    Article

    Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

    Inserts

    flp recombinase
    rhaS
    rhaR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhamnose

    Available since

    Dec. 8, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pET His6 GST TEV LIC cloning vector (2G-T)

    Plasmid

    #29707

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, GST, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lambda Phosphatase

    Plasmid

    #79748

    Purpose

    Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

    Depositor

    John Chodera, Markus Seeliger, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    Lambda Phosphatase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSBbi-Pur

    Plasmid

    #60523

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBAV1K-T5-gfp

    Plasmid

    #26702

    Depositor

    Ichiro Matsumura

    Article

    Bryksin et al PLoS One. 2010 . 5(10):e13244.

    Insert

    T5lacOlacOeGFP biobrick
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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22. DsRed-rab11 WT

    Plasmid

    #12679

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB11A (Homo sapiens) (RAB11A Human)
    

    Use

    Tags

    DsRed and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCA24N-ligase

    Plasmid

    #87741

    Purpose

    IPTG-inducible expression of T4 DNA ligase for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA-dCas9-p300 Core

    Plasmid

    #61357

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. HA Raptor

    Plasmid

    #8513

    Depositor

    David Sabatini

    Article

    Kim et al Cell 2002 Jul 26;110(2):163-75.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Tags

    ha

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA

    Plasmid

    #84474

    Purpose

    pAAV-RAM-NLS-mKate2

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Insert

    mKate2
    

    Use

    AAV

    Tags

    Nuclear localization signal of SV40 large T antig…

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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27. pEvolvR-enCas9-PolI3M-TBD

    Plasmid

    #113077

    Purpose

    Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    David Schaffer, John Dueber

    Article

    Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

    Insert

    enCas9-PolI3M-TBD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Lentiviral-S-tdTomato

    Plasmid

    #112579

    Purpose

    Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

    Depositor

    Jeffrey Rothstein

    Article

    Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

    Insert

    NLS-tdTomato-NES
    

    Use

    Lentiviral

    Tags

    nuclear export sequence and nuclear localization …

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET MBP His6 LIC cloning vector (2Cc-T)

    Plasmid

    #37237

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. phage UbiC G3BP1-GFP-GFP

    Plasmid

    #119950

    Purpose

    Lentiviral vector expressing G3BP1-GFP fusion protein

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    UbiC

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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