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165,373 results

Showing: 1521 - 1560 of 165373 results

  5. pEC3109 (pSpy1C-Plac(1)_ffluc)

    Plasmid
    #218514
    Purpose
    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    ffluc
    Use
    Luciferase
    Expression
    Bacterial
    Mutation
    deletion of mrfp cassette, mutagenesis of the RBS…
    Promoter
    Plac(1)
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    218514-plasmid-map-sequence-id-444100

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  7. pcDNA3-ICUE4

    Plasmid
    #181846
    Purpose
    Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.
    Depositor
    Jin Zhang
    Article
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Insert
    ICUE4 (RAPGEF3 Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Glutamine 122 in Epac1 domain mutated to glutamat…
    Promoter
    CMV
    Available Since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181846-plasmid-map-sequence-id-364236

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  9. pTiGc

    Plasmid
    #78314
    Purpose
    Mycobacterial dual reporter plasmid for fluorescence dilution. Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch).
    Depositor
    Samantha Sampson
    Article
    Mouton et al Microbiology. 2016 Mar 30. doi: 10.1099/
    Inserts
    TurboFP635
    GFP
    Use
    Synthetic Biology; Mycobacterial replicating vect…
    Expression
    Bacterial
    Promoter
    hsp60 and hsp60(ribo)
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. pOpen-NotIR (GC)

    Plasmid
    #165507
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    NotI (GC-rich recognition site)
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165507-plasmid-map-sequence-id-338586

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  12. hGFAP-GFP

    Plasmid
    #40592
    Depositor
    Albee Messing
    Article
    Zhuo et al Dev Biol. 1997 Jul 1;187(1):36-42.
    Insert
    GFP-S65T
    Use
    Mouse Targeting
    Tags
    MP-1 fragment (mouse protamine 1 intron and polya…
    Expression
    Bacterial and Mammalian
    Mutation
    Construct contains a mutated, humanized hGFP(S65T…
    Promoter
    hGFAP promoter
    Available Since
    Oct. 4, 2012
    Availability
    Academic Institutions and Nonprofits only
    40592-plasmid-map-sequence-id-240470

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  13. NES-Grx1-roGFP2

    Plasmid
    #236551
    Purpose
    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the cytoplasm.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    NES-Grx1-roGFP2 (NES Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236551-plasmid-map-sequence-id-469946

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  14. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104910
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    104910-plasmid-map-sequence-id-206061

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  18. pDB5050

    Plasmid
    #171126
    Purpose
    A plasmid containing the codon-optimized OsTIR1, which is under the control of adh1 promoter. It served as one of the F-box protein expression plasmids in AID system.
    Depositor
    Li-Lin Du
    Article
    Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.
    Insert
    OsTIR1
    Use
    Other
    Mutation
    non applicable
    Available Since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171126-plasmid-map-sequence-id-341097

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  20. GPRC6A-Tango

    Plasmid
    #66386
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPRC6A (GPRC6A Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66386-plasmid-map-sequence-id-331886

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  23. pGRG36-Kn-PA1-GFP

    Plasmid
    #79088
    Purpose
    pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)
    Depositor
    Frank Rosenzweig
    Article
    Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)
    Insert
    GFP
    Use
    Bacterial chromosome integration and expression o…
    Expression
    Bacterial
    Promoter
    PA1
    Available Since
    July 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    79088-plasmid-map-sequence-id-182383

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  28. Split 'n coexpress CUL3-RBX1

    Plasmid
    #52293
    Purpose
    bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1
    Depositor
    Brenda Schulman
    Article
    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1
    Inserts
    Cul3 N-terminal domain (CUL3 Human)
    Cul3 C-terminal domain (CUL3 Human)
    RBX1 (Rbx1 Mouse)
    Tags
    GST, IRES, and TEV
    Expression
    Bacterial
    Mutation
    contains aa 1-309, contains aa 310-768, and conta…
    Promoter
    IRES and tac
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  29. human ACF

    Plasmid
    #112860
    Purpose
    plasmid to overexpress human APOBEC1-complementation factor (ACF; A1CF) in mammalian cells
    Depositor
    Silvestro Conticello
    Article
    Chieca et al bioRxiv 2021
    Insert
    APOBEC1 complementation factor (A1CF Human)
    Expression
    Mammalian
    Available Since
    Oct. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    112860-plasmid-map-sequence-id-224730

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  30. pAAV2/5

    Plasmid
    #232922
    Purpose
    AAV packaging plasmid expressing AAV2 Rep protein and AAV5 capsid protein
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Rep2/Cap5
    Use
    AAV
    Expression
    Mammalian
    Available Since
    Sept. 17, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    232922-plasmid-map-sequence-id-458562

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  31. mEos4b-TOMM20-N-10

    Plasmid
    #57518
    Purpose
    Localization: Mitochondrial Membrane, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson, Loren Looger
    Insert
    TOMM20 (TOMM20 Human)
    Tags
    mEos4b
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 4, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57518-plasmid-map-sequence-id-306066

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  36. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid
    #215769
    Purpose
    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus
    Depositor
    Dimitrios Wagner
    Article
    Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:
    Inserts
    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Mouse, Human, Bovine)
    TRAC-3' homology arm (TRAC Human)
    Expression
    Bacterial
    Mutation
    Cas12a PAM mutated
    Available Since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    215769-plasmid-map-sequence-id-435524

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Showing: 1521 - 1560 of 165373 results