We narrowed to 2,443 results for: CLU
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Plasmid#239753PurposeTemplate for in vitro transcription of nanoluciferase flanked by the human haemoglobin beta 5' and 3' UTRs.DepositorInsertNanoluciferase flanked by human haemoglobin beta 5' and 3' UTRs
UseLuciferasePromoterT7 (included as part of insert)Available SinceJuly 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCX-SpiCee-NES
Plasmid#140901Purposemammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signalingDepositorInsertSpiCee-NES
TagsmRFPExpressionMammalianPromoterCAGAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM376 - sgRNA - spc4 - array - insertion - unc-119 - locus
Plasmid#182344PurposesgRNA4 for insertion of extrachromosomal arrays into the ce-unc-119 genomic locus. Use with pSEM371 fragment included in Ex array.DepositorInsertSpacer 4
ExpressionWormAvailable SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWJB9
Plasmid#126650PurposeExpresses AdrA from a modified pLAFR3DepositorInsertadrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30
Tags8xHisExpressionBacterialPromoterPBADAvailable SinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrc99a-NoSS-TolC
Plasmid#155179PurposeExpresses signal sequence-less TolC as inclusion bodiesDepositorInsertTolC (tolC )
ExpressionBacterialMutationdeleted residues 1-20, residue 21 mutated to Met …PromotertrcAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZS*11_4enh
Plasmid#149423PurposePlasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).DepositorInsertsrbcS-E9 enhancer
35S core enhancer
Cab-1 enhancer
AB80 enhancer
TagsT120>AExpressionBacterialAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-dALFANb(3mut)-mGreenLantern
Plasmid#219662PurposeGal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLanternDepositorInsertDestabilised ALFA nanobody (3 mutations) fused to mGreenLantern
ExpressionInsectAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSG5-ERRbeta2
Plasmid#52186PurposeExpresses human ERRbeta2 splice variant in mammalian cellsDepositorInsertEstrogen-related receptor beta (ESRRB Human)
ExpressionMammalianMutationwild type sequence; codon optimized to exclude Ec…PromoterSV40Available SinceApril 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-iRFP-B1-timestamp_A
Plasmid#159924PurposeTimestamp Template A inserted into the 3’ UTR of iRFP between bActin Zipcode element and WPRE, with RNA barcode TGC. Also includes a xrRNA element in the 5’ UTR. AKA 147B1.DepositorInsertiRFP-Timestamp A
ExpressionMammalianPromoterTRE3GAvailable SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTS2340_Tier3(SB)-iRFP_Puro
Plasmid#169638PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven iRFP670 and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFB-LIC-Bse
Plasmid#26108PurposeSGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selectionDepositorTypeEmpty backboneUseBaculovirus expressionTagsHis6 - TEVExpressionInsectPromoterpolyhedrinAvailable SinceOct. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCX-SpiCee-Kras
Plasmid#140838Purposemammalian expression of SpiCee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the calcium buffer SpiCee that prevents downstream signalingDepositorInsertSpiCee-Kras
TagsmRFPExpressionMammalianPromoterCAGAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Agrp-pGL3
Plasmid#17552DepositorAvailable SinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Pdgfra-T2A-H2B-eGFP donor
Plasmid#113120PurposeDonor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequenceDepositorInsertPdgfra donor region including homology arms and T2A-H2B-eGFP (Pdgfra Mouse, Synthetic)
UseSynthetic BiologyAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21c(+)-EFG1mt
Plasmid#31076DepositorInsertMature form human mitochondrial elongation factor G isoform 1 (GFM1 Human)
TagsHisExpressionBacterialMutationIncludes the coding sequence for the mature form.…Available SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTn7·X-nia
Plasmid#122592PurposePlasmid for genomic integraction of xylS-Pm->nia into the Tn7 insertion siteDepositorInsertxylS/Pm->Nia
UseSynthetic Biology; Tn7 genomic integrationExpressionBacterialPromoterPmAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiMutB3GALT5-blast
Plasmid#208381Purposelentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequenceDepositorInsertbeta-1,3-galactosyltransferase 5 (B3GALT5 Human)
UseLentiviralTagsmyc, FLAGExpressionMammalianMutationnucleotide change in PAM targeting sequence nt 51…PromoterEF-1 alpha core promoterAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101 NL4-3 envFS Δrev ΔRRE RTEm26CTE
Plasmid#101351PurposeHIV-1 delta Rev was cloned into pSC101 and modified to include an RTEm26CTE element in order to utilize the NXF1 RNA export pathway. The RRE was also mutated.DepositorInsertNL4-3
UseLentiviralAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDM098
Plasmid#216818PurposeAspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.DepositorInsertmStayGold
TagsFLAG-(SGGS)x2-XTEN16-(GGGGS)x3ExpressionBacterialMutationAspergillus nidulans codon-adjustedAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1R1
Plasmid#97323PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1R1
Tags6xHis - TEVExpressionBacterialMutationAddition of R residue after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
1456_pDEST_miniTol2_R4-R2_Cryst-eGFP
Plasmid#171795PurposepDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-dCas9VPH-T2A-GFP-shP53
Plasmid#102895PurposeEBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.DepositorInsertdCas9VPH-T2A-GFP-shP53
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2SCb
Plasmid#59385PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.DepositorInsertTT-ISceI-cat2
UseTemplateMutationThe 3’-end of the chloramphenicol resistance gene…Available SinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1G1
Plasmid#97325PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1G1
Tags6xHis - TEVExpressionBacterialMutationAddition of G residue after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-EEA-guide1-PGK-Puro
Plasmid#102904PurposeEBNA episome plasmid for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.DepositorInsertEEA-guide1-PGK-Puro
UseCRISPRExpressionMammalianPromoterU6Available SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRKB134_GI-DUX4-intron2(-)
Plasmid#83968Purposeto test DUX4 mRNA is subjected to NMD upon intron2 inclusionDepositorAvailable SinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRKB133_GI-DUX4-intron2(+)
Plasmid#83967Purposeto test DUX4 mRNA is subjected to NMD upon intron2 inclusionDepositorAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-AtMIR390a-NbSu-2
Plasmid#213400PurposePlant expression vector (2x35S) for expressing an amiRNA against Nicotiana benthamiana SULFUR gene from AtMIR390a precursorDepositorInsertArabidopsis MIR390a precursor including amiRNA/amiRNA* sequences for silencing N. benthamiana SULFUR gene
ExpressionPlantPromoter2x35SAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABEhygro-Flag-ZO-2
Plasmid#27473DepositorAvailable SinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-Flag-ZO-2
Plasmid#27420DepositorAvailable SinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTS2337_Tier3(SB)-TagBFP2_Puro
Plasmid#169635PurposeTier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase including a TagBFP marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven mTagBFP2 and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458_miR-290-295KO-gRNA1
Plasmid#172709PurposeCas9 with 5' gRNA for paired CRISPR KO of miR-290-295 clusterDepositorInsertmiR-290-295
UseCRISPRAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458_miR-290-295KO-gRNA2
Plasmid#172710PurposeCas9 with 3' gRNA for paired CRISPR KO of miR-290-295 clusterDepositorInsertmiR-290-295
UseCRISPRAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-ZapCY2
Plasmid#59016PurposeCytosolic zinc-binding FRET biosensorDepositorInsertNES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
TagsNuclear Exclusion SignalExpressionMammalianMutationSee commentPromoterCMVAvailable SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIBA3-SpyCatcherEQ-sfGFP
Plasmid#107421PurposeFor production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.DepositorInsertSpyCatcher
TagsHis tag, StrepII, and superfolder GFPExpressionBacterialMutationmutation of glutamate 77 to glutaminePromoterTetAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIBA2-SpyTag-MBP
Plasmid#107422PurposeControl construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.DepositorInsertMaltose-binding protein (MalE)
TagsOmpA signal peptide, SpyTag, and StrepIIExpressionBacterialPromoterTetAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTS2353_Tier3(PB)-YPet_PuroR
Plasmid#169652PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven YPet and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOmpT
Plasmid#112123PurposeBacterial expression outer membrane protein T (OmpT), wild typeDepositorInsertOmpT (ompT E. coli)
ExpressionBacterialMutationN-terminal signal sequence not included and repla…PromoterT7Available SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOmpW
Plasmid#112121PurposeBacterial expression of outer membrane protein W (OmpW), wild typeDepositorInsertOmpW (ompW E. coli)
ExpressionBacterialMutationN-terminal signal sequence not included and repla…PromoterT7Available SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTH353-SUP45wt
Plasmid#29371DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
ExpressionYeastMutationWild type gene including genomic sequence 1432 bp…Available SinceMay 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
UR1Ct (265-1040)
Plasmid#115647Purposeexpression of truncated Rag1 including the RING, E3 ubiquitin ligase (265-1008) and C-terminal region (384-1040)DepositorInsertRag1 (265-1040) (Rag1 Mouse)
Tags8XHis-MBP-PreScissionExpressionMammalianMutationtruncated Rag1 (aa 265-1040)Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
gRD21A-mRFP
Plasmid#181959PurposeRD21A protein tagged with mRFP, under control of native promoterDepositorInsertRD21A genomic sequence including 2kb upstream of the ATG
TagsmRFPExpressionPlantPromoterRD21A nativeAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pProvector_AAV2_586_PLGLAR
Plasmid#129024PurposeRecombinant AAV2 rep/cap plasmid modified to include an 18 amino acid peptide at cap position 586.DepositorInsertRep2/Cap2 with PLGLAR lock at 586
UseAAVMutationinserted peptide motif (PLGLARGDDDDGPLGLAR) at po…Available SinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-TdT-guide1-PGK-Puro
Plasmid#102903PurposeEBNA episome plasmid for U6 promoter-driven expression of a control gRNA targeting TdTomato sequence. Includes PGK-puro selection cassette.DepositorInsertTdT-guide1-PGK-Puro
UseCRISPRExpressionMammalianPromoterU6Available SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF
Plasmid#72949PurposeEntry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction siteDepositorInsertLevel 0 - ORFs
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSUM-GroEL-gfp
Plasmid#109286PurposeExpresses gfp in mycobacteriaDepositorInsertGroEL-GFP
ExpressionBacterialMutationGFP modified for expression in prokaryotes; inclu…PromoterGroELAvailable SinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1T1
Plasmid#97327PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1T1
Tags6xHis - TEVExpressionBacterialMutationAddition of T residue after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2Flag CMV2-ZO-2-1st-PDZ-mut
Plasmid#27416DepositorInsertZonula Occludens 2 (TJP2 Human)
Tags2xFlagExpressionMammalianMutationK38E, F44H, I46E and V48EAvailable SinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMT1100
Plasmid#8618DepositorInsertribonuclease Sa3, barstar
TagsphoA signal sequence to RNase Sa3ExpressionBacterialMutationIn addition to the substitution of the barnase ge…Available SinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T3-ZO-2-1st-PDZ
Plasmid#27424DepositorAvailable SinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only