Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

164,243 results

Showing: 61 - 80 of 164243 results

  2. LMAN1-bio-His

    Plasmid
    #51922
    Purpose
    Expresses full-length ERGIC-53 protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    LMAN1 (LMAN1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51922-plasmid-map-sequence-id-79902

    Enlarge

  3. pcDNA3.3_SARS2_JN.1

    Plasmid
    #218678
    Purpose
    expressing SARS-CoV-2 JN.1 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (JN.1)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L455S on the basis of BA.2.86
    Promoter
    CMV
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    218678-plasmid-map-sequence-id-437262

    Enlarge

  4. pExp-MBP

    Plasmid
    #112568
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-MBP and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112568-plasmid-map-sequence-id-217224

    Enlarge

  8. pExp-NusA

    Plasmid
    #112571
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112571-plasmid-map-sequence-id-220666

    Enlarge

  13. pAAV.hSynap.iAChSnFR

    Plasmid
    #137950
    Purpose
    Expresses iAChSnFR under hSynapsin promoter
    Depositor
    Loren Looger
    Article
    Borden et al bioRxiv 2020
    Has Service
    AAV1
    Insert
    iAChSnFR
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSynanpsin
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    137950-plasmid-map-sequence-id-271681

    Enlarge

  17. pCAG-Roxed-Cre

    Plasmid
    #51273
    Purpose
    Component of the Co-Driver binary site-specific recombinase system, generates functional rox60-71Cre upon removal of an internal STOP cassette by Dre
    Depositor
    Pawel Pelczar
    Article
    Hermann et al Nucleic Acids Res. 2014 Jan 9.
    Insert
    Roxed-Cre
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51273-plasmid-map-sequence-id-77891

    Enlarge

  20. pMAL-SH3(2)-5R

    Plasmid
    #112089
    Purpose
    Bacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.
    Depositor
    Michael Rosen
    Article
    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi
    Insert
    SH3(2)-5R (NCK1 Human)
    Use
    Tags
    His6 and MBP
    Expression
    Bacterial
    Mutation
    construct contains only repeats of the second SH3…
    Promoter
    Lac
    Available Since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112089-plasmid-map-sequence-id-236082

    Enlarge

Showing: 61 - 80 of 164243 results