161,398 results

1. pGS-21a his6-GST-WTPhi29DNAP

   Plasmid

   #166863

   Purpose

   To produce a dual affinity-tagged Phi29 DNA polymerase

   Depositor

   Paul Blainey, Stephen Quake

   Article

   Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

   Insert

   Phi29 Polymerase codon-optimized for overexpression in E. coli
   

   Use

   Tags

   GST and his6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDNA-FLAG-METTL3-APPA

   Plasmid

   #160251

   Purpose

   Express catalytic inactive FLAG-METTL3 in human cells

   Depositor

   Alessandro Fatica

   Article

   Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changes in aa 395–398, DPPW → APPA

   Promoter

   CMV

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pT3-EF1a-YapS127A

   Plasmid

   #46049

   Depositor

   Xin Chen

   Article

   Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   S127A

   Promoter

   EF1a

   Available since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pGP-AAV-syn-jGCaMP8s-WPRE

   Plasmid

   #162374

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8s
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   S26M F286Y Q315H

   Promoter

   Synapsin

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. BPK3082

   Plasmid

   #78742

   Purpose

   Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   LbCpf1 crRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-Delta(Pro-MP)ADAM10-HA

   Plasmid

   #65107

   Purpose

   Expression of HA tagged ADAM10 lacking prodomain and metalloprotease domain in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

   Insert

   Delta(Pro-MP)ADAM10 (ADAM10 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deleted AA 19-455

   Promoter

   CMV

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

7. DLX2-hygro

   Plasmid

   #97330

   Purpose

   Generating lentiviruses to express Dlx2 and hygromycin resistance

   Depositor

   Marius Wernig

   Article

   Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

   Insert

   DLX2 (Dlx2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TetO

   Available since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-hSyn-hChR2(H134R)-mCherry

   Plasmid

   #26976

   Purpose

   AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation

   Depositor

   Karl Deisseroth

   Has Service

   AAV5, AAV8, and AAV9

   Insert

   hChR2(H134R)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   hSyn

   Available since

   Feb. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMIIS948-CXCR4

   Plasmid

   #238213

   Purpose

   TasR tigRNA expression to target CXCR4 under U6 promoter in human cells.

   Depositor

   Feng Zhang

   Article

   Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

   Insert

   tigRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-MBP-mSA2

    Plasmid

    #52319

    Purpose

    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

    Depositor

    Sheldon Park

    Article

    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

    Insert

    monomeric streptavidin 2
    

    Use

    Tags

    FLAG, His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    S25H mutation compared to streptavidin

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GFP-polyQ23-NLS

    Plasmid

    #231891

    Purpose

    Expression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleus

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q23-NLS (HTT Human)
    

    Use

    Tags

    EGFP and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-mPCSK7

    Plasmid

    #122676

    Purpose

    Expresses mouse PCSK7 in mammalian cells

    Depositor

    Liming Pei

    Article

    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

    Insert

    Mus musculus proprotein convertase subtilisin/kexin type 7 (Pcsk7), transcript variant 2 (Pcsk7 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-hSyn-hChR2(H134R)-EYFP

    Plasmid

    #26973

    Purpose

    AAV expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic excitation

    Depositor

    Karl Deisseroth

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    hSyn

    Available since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-TRE-ChR2-YFP

    Plasmid

    #171622

    Purpose

    TRE-ChR2-YFP reporter

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    TRE-ChR2-YFP
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28-mE1

    Plasmid

    #32534

    Depositor

    Jorge Eduardo Azevedo

    Article

    Carvalho et al Mol Biotechnol. 2011 Oct 20.

    Insert

    E1, ubiquitin-activating enzyme (Uba1 Mouse)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-SnoopCatcher

    Plasmid

    #72322

    Purpose

    Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a-SnoopCatcher
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed G842T and D848G of S. pneumoniae adhesin …

    Promoter

    T7

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mito-meGFP

    Plasmid

    #172481

    Purpose

    meGFP targeted to the mitochondrial matrix with a human COX8 presequence

    Depositor

    Thomas Schwarz

    Insert

    Mito
    

    Use

    Tags

    meGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSLPB2B-FKBP12_F36V-CasRx-tagBFP-PGK-Blasticidin

    Plasmid

    #187952

    Purpose

    FKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-CasRx-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker, HA-tag, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hLRP1-pEZT-BM

    Plasmid

    #177985

    Purpose

    Expression the full-length ectodomain of human LRP1 [residue 1-4414] in mammalian cells

    Depositor

    Lora Hooper

    Article

    Bang et al Science. 2021 Sep 17;373(6561):eabf9232.

    Insert

    LDL receptor related protein 1 (LRP1 Human)
    

    Use

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ABE8r-V106W

    Plasmid

    #208295

    Purpose

    Expresses ABE8r in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r-V106W-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106W, …

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge