165,385 results

1. hAAVS1-gs4_EF1a-Cas9-2A-GFP

   Plasmid

   #118414

   Purpose

   CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

   Depositor

   Mark Denham

   Article

   Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

   Insert

   gRNA AAVS1 (AAVS1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. mEGFP-8xbiRhoBAST

   Plasmid

   #196351

   Purpose

   Expression of mEGFP mRNA with 8xbiRhoBAST in 3'UTR

   Depositor

   Murat Sunbul

   Article

   Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

   Insert

   mEGFP-8xbiRhoBAST
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLL5.0-MRLC-WT-GFP

   Plasmid

   #187258

   Purpose

   Lentiviral expression of myosin regulatory light chain-WT

   Depositor

   Alpha Yap

   Article

   Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

   Insert

   MRLC1 (MYL9 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Promoter

   5' LTR

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2+8CmCherry

   Plasmid

   #34935

   Depositor

   Amro Hamdoun

   Article

   Gokirmak et al J Biol Chem. 2012 Nov 2.

   Type

   Empty backbone

   Use

   Sea urchin, zebrafish, xenopus

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   SP6

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. H52-pUCM-hNGN2-inv

   Plasmid

   #110492

   Purpose

   Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm

   Depositor

   Michael Ward

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   mcherry
   rtTA3G
   

   Use

   CRISPR, Cre/Lox, and TALEN

   Expression

   Mammalian

   Promoter

   CAG, EF-1 alpha, and TRE3G

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRRL.SP146.gp47.eGFP

   Plasmid

   #30475

   Depositor

   Didier Trono

   Article

   Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

   Insert

   SP146/gp47
   

   Use

   Lentiviral

   Available Since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUF-Cas9-pre-sgRNA

   Plasmid

   #137845

   Purpose

   Cas9 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

   Depositor

   Josh Beck

   Article

   Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   May 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-myc-Rac1-T17N

   Plasmid

   #12984

   Depositor

   Gary Bokoch

   Insert

   Rac1 DN (RAC1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   T17N Dominant negative

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_CHD2_WT

   Plasmid

   #81768

   Purpose

   Gateway Donor vector containing CHD2 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   CHD2 (CHD2 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn1-tdTomatof

    Plasmid

    #104060

    Purpose

    Also known as hSyn1-tdTomato-f. AAV expression of Farnylsated tdtomato for tracing

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    tdTomato
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-SspB-mScarlet-I

    Plasmid

    #178521

    Purpose

    Expresses SspB-mScarlet-I in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    SspB-mScarlet-I
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBABE-hygro-2xMyc-Rac1-WT

    Plasmid

    #128580

    Purpose

    For mammalian cell expression of WT murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

    Depositor

    Julian Downward

    Article

    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

    Insert

    Rac1 (Rac1 Mouse)
    

    Use

    Retroviral

    Tags

    2xMyc epitope

    Expression

    Mammalian

    Available Since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRG01-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-WPRE

    Plasmid

    #123362

    Purpose

    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance and Firefly luciferase.

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Methods. 2019 May;16(5):405-408. doi

    Insert

    Firefly luciferase
    

    Use

    CRISPR, Lentiviral, and Luciferase

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBABE-hygro-2xMyc-Rac1-P29S

    Plasmid

    #128581

    Purpose

    For mammalian cell expression of P29S mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

    Depositor

    Julian Downward

    Article

    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

    Insert

    Rac1 (Rac1 Mouse)
    

    Use

    Retroviral

    Tags

    2xMyc epitope

    Expression

    Mammalian

    Mutation

    Changed Proline29 to Serine.

    Available Since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. YEpADE1

    Plasmid

    #22495

    Depositor

    Beth Montelone

    Insert

    ADE1 (ADE1 Budding Yeast)
    

    Use

    Yeast shuttle

    Mutation

    Original 4067 bp HindIII-HindIII insertion (see Y…

    Available Since

    Jan. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSR62

    Plasmid

    #125076

    Purpose

    Expression of ompR under PLtetO-1, output promoter PompF146

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    ompR (ompR )
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. mEGFP-2xbiRhoBAST

    Plasmid

    #196349

    Purpose

    Expression of mEGFP mRNA with 2xbiRhoBAST in 3'UTR

    Depositor

    Murat Sunbul

    Article

    Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

    Insert

    mEGFP-2xbiRhoBAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIRES2-DsRed-MST-Kv2.1

    Plasmid

    #131707

    Purpose

    Expresses Kv2.1in mammalian cells

    Depositor

    Jennifer Kearney

    Article

    Jorge et al Proc Natl Acad Sci U S A. 2011 Mar 29;10

    Insert

    KCNB1 (KCNB1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mEGFP-4xbiRhoBAST

    Plasmid

    #196350

    Purpose

    Expression of mEGFP mRNA with 4xbiRhoBAST in 3'UTR

    Depositor

    Murat Sunbul

    Article

    Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

    Insert

    mEGFP-4xbiRhoBAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEE392

    Plasmid

    #149282

    Purpose

    T-DNA encoding TRV2 with mFT augmented gRNA targeting NbPDS3

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbPDS3sgRNA_mFT:tNos
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

21. psd44-G13WT

    Plasmid

    #46829

    Purpose

    expression of wild type G protein alpha 13 in mammalian cells

    Depositor

    Agnese Mariotti

    Insert

    GNA13 (GNA13 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK promoter

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

22. Anti-Kv2.1 K+ channel (External) [K39/25R]

    Plasmid

    #128620

    Purpose

    Mammalian Expression Plasmid of anti-Kv2.1 K+ channel (External) (Human). Derived from hybridoma K39/25.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Kv2.1 K+ Channel (External) (Human) recombinant mouse monoclonal antibody (KCNB1 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pT7-EGFP-C1-HsDCP2-E148Q_U

    Plasmid

    #147650

    Purpose

    Mammalian Expression of HsDCP2-E148Q

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsDCP2-E148Q (DCP2 Human)
    

    Expression

    Mammalian

    Mutation

    two silent mutations compared to the sequence giv…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRRL.SP146.gp91.eGFP

    Plasmid

    #30474

    Depositor

    Didier Trono

    Article

    Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

    Insert

    SP146/gp91/eGFP
    

    Use

    Lentiviral

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GOLPH3 shRNA

    Plasmid

    #163860

    Purpose

    shRNA targeting the coding sequences of GOLPH3

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    golgi phosphoprotein 3 (GOLPH3 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGWB440

    Plasmid

    #74826

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Anti-GABA(B)R2 [N81/37.1R]

    Plasmid

    #114544

    Purpose

    Mammalian Expression Plasmid of anti-GABA(B)R2 (Human). Derived from hybridoma N81/37.1.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-GABA(B)R2 (Homo sapiens) recombinant mouse monoclonal antibody (GABBR2 Mouse)
    

    Expression

    Mammalian

    Promoter

    dual CMV

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRFP

    Plasmid

    #26924

    Depositor

    Ron Weiss

    Article

    Basu et al Nature. 2005 Apr 28. 434(7037):1130-4.

    Insert

    LacI, HcRed
    

    Expression

    Bacterial

    Available Since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBABE-hygro-2xMyc-Rac1-Q61L

    Plasmid

    #128582

    Purpose

    For mammalian cell expression of Q61L mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

    Depositor

    Julian Downward

    Article

    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

    Insert

    Rac1 (Rac1 Mouse)
    

    Use

    Retroviral

    Tags

    2xMyc epitope

    Expression

    Mammalian

    Mutation

    Changed Glutamine61 to Leucine.

    Available Since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV.SP146.gp91.eGFP.cHS4

    Plasmid

    #30476

    Depositor

    Didier Trono

    Article

    Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

    Insert

    SP146/gp91/eGFP/cHS4
    

    Use

    Lentiviral

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge