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167,150 results

Showing: 121 - 150 of 167150 results

  4. pIRES-EGFP-puro

    Plasmid
    #45567
    Depositor
    Michael McVoy
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    IRES-EGFP-Puro
    Expression
    Mammalian
    Promoter
    Phcmv (strong human cytomegalovirus immediate ear…
    Available Since
    June 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    45567-plasmid-map-sequence-id-237092

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  6. hNTa1-qgRNA-pYJA5

    Plasmid
    #217779
    Purpose
    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    217779-plasmid-map-sequence-id-466021

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  11. pGL4.10/RSV_SF3B3-miniXon_Luciferase

    Plasmid
    #174660
    Purpose
    Plasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.
    Depositor
    Beverly L. Davidson
    Article
    Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586
    Insert
    miniXon cassette
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Kozak ATG initiation sequence in middle exon
    Promoter
    RSV
    Available Since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    174660-plasmid-map-sequence-id-351622

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  16. pDG458

    Plasmid
    #100900
    Purpose
    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100900-plasmid-map-sequence-id-457690

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  29. pIRES Centrin1 mCherry

    Plasmid
    #64338
    Purpose
    Bicistronic expression of mCherry-Centrin1 fusion and Hygro resistance gene
    Depositor
    Matthieu Piel
    Insert
    Centrin1
    Tags
    IRES-Hygro and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    64338-plasmid-map-sequence-id-227446

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Showing: 121 - 150 of 167150 results