We narrowed to 5,536 results for: Chia
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Plasmid#105815PurposeExpresses active LwaCas13a-NES for mammalian RNA targetingDepositorInsertLwCas13a
UseCRISPR and LentiviralTagsNES and msfGFP-NES-3xHAExpressionMammalianMutationPromoterEF-1aAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC014 - LwCas13a-msfGFP
Plasmid#91902PurposeExpresses active LwCas13a for mammalian RNA targetingDepositorInsertLwCas13a
UseCRISPRTagsNLS and msfGFP-NLS-3xHAExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-GFP-P2A-NptII-9
Plasmid#197741PurposePhytobrick (MoClo) Level 0 PartDepositorInsertEGFP-P2A-NptII bicistronic CDS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKL001
Plasmid#98917PurposeConstitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.DepositorInsertCaPR
UseTagsExpressionBacterialMutationPromoterBBa_J23118 - Strong ConstitutiveAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
Plasmid EUPp_09
Plasmid#157745PurposePgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)DepositorInsertsUseTagsExpressionBacterialMutationPromoterPgyrAAvailable sinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 rmtB
Plasmid#112880PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertrmtB (RmtB Escherichia coli)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-BSA4-G-L11
Plasmid#127830PurposeProduction of recombinant human ribosomal protein L11DepositorInsertRPL11 (RPL11 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pTA-Mob 2.0
Plasmid#149662PurposeConjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.DepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC003 - LshC2C2 locus into pACYC184 for spacer cloning
Plasmid#79152PurposeLshC2c2 locus in pACYC184 with BsaI sites for spacer cloningDepositorInsertLshC2c2 locus
UseTagsExpressionMutation*PromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE-SHH
Plasmid#134350PurposeTargeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locusDepositorInsertSHH (SHH Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)
Plasmid#110559PurposeminiTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoterDepositorInsertrhaSR-PrhaBAD
UseTagsExpressionBacterialMutationPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCB306-GFP-Rab12
Plasmid#198471Purposelentiviral expression of GFP-Rab12DepositorInsertGFP-Rab12 (RAB12 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterEF1AAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21a - Ec-ClpX
Plasmid#111516PurposeExpresses E. coli ClpX with a C-terminal His tagDepositorInsertClpX
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMS_366
Plasmid#182131Purposeexpresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutationDepositorInsertsretron RNA
ec.86 RT
CspRecT
UseTagsExpressionMutationincorporated rifampicin-resistance-conferring don…PromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)
Plasmid#73404PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsAt4CL
PhCHS
CmCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-2-EWSR1
Plasmid#46384DepositorInsertEWSR1 (EWSR1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT-G-L11
Plasmid#127831PurposeProduction of recombinant human ribosomal protein L11DepositorInsertRPL11 (RPL11 Human)
UseTagsHis6 - Thioredoxin - TEVExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pJM101(pUC18T-miniTn7T-gm-lacIq-Ptac)
Plasmid#110558PurposeminiTn7 delivery plasmid with lacIq-Ptac inducible promoterDepositorInsertlacIq-Ptac
UseTagsExpressionBacterialMutationPromoterlacIq-PtacAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
RBD#4L
Plasmid#193021PurposehnRNP C RRM RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13aDepositorInsertCas13a
UseTagshnRNP C RRMExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBAD-yhjH
Plasmid#111613PurposeArabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjHDepositorInsertyhjH
UseTagsExpressionBacterialMutationA52VPromoterAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-N-TEV-Twin-Strep
Plasmid#202526PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cellDepositorTypeEmpty backboneUseTagsTEV cleavable site and Twin-strep-tagExpressionMammalianMutationPromoterCAGAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A
Plasmid#83485PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).DepositorInsertLbu_C2c2_R472A_H477A_R1048A_H1053A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR472A, H477A, R1048A, H1053APromoterAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA_J23119-sgRNA
Plasmid#108550PurposeBacterial Target-AID vector with sgRNADepositorInsertsdCas9-PmCDA1
Streptococcus pyogenes sgRNA
UseTagsFlagExpressionBacterialMutationD10A and H840A for SpCas9PromoterJ23119 and lambda ORAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFDH
Plasmid#131706PurposeExpress constitutively an NAD-dependent formate dehydrogenase from Pseudomonas sp. 101 in E. coliDepositorInsertformate dehydrogenase
UseTagshis-tagExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046887
Plasmid#145730PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGGS-C-TEV-Twin-Strep
Plasmid#202527PurposeExpress N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cellDepositorTypeEmpty backboneUseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTU1-B-SP44s-GUS
Plasmid#172430PurposeExpresses Streptomyces codon-optimised beta-D-glucuronidaseDepositorInsertbeta-D-glucuronidase
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterSP44sAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15ABAD- YFP
Plasmid#186571Purposep15A ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterBAD promoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEC3079 (p7INT-Pveg_lacI)
Plasmid#218505Purposeintegrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integraseDepositorInsertlacI (lacI Escherichia coli)
UseTagsExpressionBacterialMutationPromoterPvegAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pTJV1Sc-rpoB
Plasmid#166978PurposeGenerates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistanceDepositorInsertsUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23101 and VanCCAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-AP1/gamma1
Plasmid#218801PurposeMammalian expression of mouse Ap1 g1DepositorInsertAp1 g1 (Ap1g1 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15-His-∆NGlpG
Plasmid#155131PurposeExpresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15bDepositorInsertGlpG (glpG Escherichia coli)
UseTagsHisExpressionBacterialMutationinsert codes for GlpG residues 87-276PromoterT7Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
RBD#3L
Plasmid#193020PurposehnRNP A1 RRM2 RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13aDepositorInsertCas13a
UseTagshnRNP A1 RRM2ExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF13B
Plasmid#134626PurposeMammalian expression of mouse KIF13B 442-1843DepositorInsertKIF13B (Kif13b Mouse)
UseTagseGFPExpressionMammalianMutationWTPromoterChicken Beta actinAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET17b-yebST
Plasmid#175804PurposeComplementation of yebST knockout in E. coli K-12DepositorInsertyebST
UseTagsNoneExpressionBacterialMutationsilent mutations to introduce restriction sitesPromoterT7Available sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKK233-hCaM
Plasmid#47598DepositorInsertCaM (CALM3 Human)
UseTagsExpressionBacterialMutationcontains 469 bp fragment (nt 92-559)PromotertrcAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast
Plasmid#213117PurposeExpression of a faster-running MinE mutant in mammalian cellsDepositorInsertMinE(L4E)-EGFP (minE Escherichia coli)
UseLentiviralTagsExpressionMammalianMutationL4EPromoterEF1aAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-FRB-mCherry-MinD-IRES-Puro
Plasmid#213116PurposeExpression of FRB-mCherry-MinD in mammalian cells for payload deliveryDepositorInsertFRB-mCherry-MinD (minD Escherichia coli)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-FEN1DA
Plasmid#35028DepositorInsertFEN1DA (FEN1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutationD181A--NUCLEASE DEAD POINT MUTANTPromoterCMVAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJM100 (pUC18T-miniTn7T-gm-araC-ParaBAD)
Plasmid#110554PurposeminiTn7 delivery plasmid with araC-ParaBAD inducible promoterDepositorInsertaraC-ParaBAD
UseTagsExpressionBacterialMutationPromoterAraC-ParaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)
Plasmid#171129PurposeLwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)DepositorInsertU6 promoter entry plasmid for LwaCas13a crRNA cloning
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFEndoNA
Plasmid#174724PurposeExpresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.DepositorInsertGFP-endoNA fusion protein
UseTagsHistagExpressionBacterialMutationPromoterT5Available sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-MinD_MinE
Plasmid#133621PurposeExpression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-FEN1deltaP
Plasmid#35030DepositorInsertFEN1DELTAP (FEN1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutation8 AMINO ACID DELETION OF P BOX (amino acids 337–3…PromoterCMVAvailable sinceSept. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCI syn iGluSnFR
Plasmid#106123PurposeIndicator for glutamate imaging (synapsin promoter)DepositorInsertiGluSnFR
UseTagsMyc and PDGFRExpressionMammalianMutationPromotersynapsinAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDIV300
Plasmid#204968PurposeAMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance markerDepositorInsertsMad7
hph (hygromycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only