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Showing: 2121 - 2160 of 4065 results

  2. pdCas9-DNMT3A-EGFP

    Plasmid
    #71666
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71666-plasmid-map-sequence-id-129527

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  6. SuperNova/pRSETB

    Plasmid
    #53234
    Purpose
    monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
    Depositor
    Takeharu Nagai
    Article
    Takemoto et al Sci Rep. 2013;3:2629. doi: 10.1038/srep0
    Insert
    monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
    Use
    Tags
    6xHis, T7 tag, and Xpress tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    53234-plasmid-map-sequence-id-236010

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  13. Lamp1-YFP

    Plasmid
    #1816
    Purpose
    Mammalian expression of Lamp1 fused to YFP. Used for localization to the lysosome.
    Depositor
    Walther Mothes
    Article
    Sherer et al Traffic 2003 Nov;4(11):785-801.
    Insert
    lysosome associated membrane protein 1 (Lamp1 Rat)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    D50E (not important for function of plasmid)
    Promoter
    Available since
    Feb. 16, 2006
    Availability
    Academic Institutions and Nonprofits only
    1816-plasmid-map-sequence-id-364671

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  24. pDEST51-LRRK2-G2019S

    Plasmid
    #29401
    Depositor
    Mark Cookson
    Insert
    LRRK2 (LRRK2 Human)
    Use
    Destination vector
    Tags
    6XHis and V5
    Expression
    Mammalian
    Mutation
    Gly2019Ser
    Promoter
    Available since
    April 1, 2011
    Availability
    Academic Institutions and Nonprofits only
    29401-plasmid-map-sequence-id-256667

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  29. pVITRO1-dV-IgG1/κ

    Plasmid
    #52213
    Purpose
    Human immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin gamma 1 constant region
    Human immunoglobulin kappa constant region
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    52213-plasmid-map-sequence-id-322304

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Showing: 2121 - 2160 of 4065 results