4,133 results

1. pcDNA3-DN-hCUL1-FLAG

   Plasmid

   #15818

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005;399:287-309. doi:

   Insert

   Cullin1 (CUL1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N452

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPTN-EGFP

   Plasmid

   #27052

   Depositor

   Beatrice Yue

   Article

   Park et al Am J Pathol. 2006 Dec . 169(6):1976-89.

   Insert

   optineurin (OPTN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Tag5Amyc-GSK3b WT

   Plasmid

   #16260

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

   Insert

   GSK3 beta (GSK3B Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHIV-Zsgreen

   Plasmid

   #18121

   Depositor

   Zena Werb, Bryan Welm

   Article

   Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

   Insert

   Zsgreen
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Pcr4-12xMBS-PBS

   Plasmid

   #52984

   Purpose

   provide MBS-PBS alternating sequence

   Depositor

   Robert Singer

   Article

   Wu et al Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038

   Insert

   12xMBS-PBS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   June 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. lentiGuide-Hygro-dTomato

   Plasmid

   #99376

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-dTomato
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hU6

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-CRY2-mCherry

   Plasmid

   #58368

   Purpose

   Mamlian codon optimized CRY2

   Depositor

   Won Do Heo

   Article

   Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

   Insert

   CRY2[1-498] (CRY2 Mustard Weed)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV Blast empty (w263-1)

   Plasmid

   #17486

   Purpose

   3rd gen lentiviral empty control vector, CMV promoter, Blast 

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pmTurquoise2-Tubulin

   Plasmid

   #36202

   Purpose

   In vivo visualization of microtubules (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   Alpha-tubulin (TUBA1B Human)
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFUGW-H1 empty vector

    Plasmid

    #25870

    Purpose

    3rd generation lentiviral vector

    Depositor

    Sally Temple

    Article

    Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    Aug. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato

    Plasmid

    #138610

    Purpose

    Express TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backbone

    Depositor

    Fei Chen

    Article

    Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Inserts

    Hyperactive AID
    T7 RNA Polymerase
    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-SARS-CoV-2-S-RBD-sfGFP

    Plasmid

    #141184

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Has Service

    Cloning Grade DNA

    Insert

    S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
    

    Use

    Tags

    Signal peptide from influenza HA and Superfolder …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHRIG-Akt1

    Plasmid

    #53583

    Purpose

    lentiviral expression of constitutively active human Akt1 and EGFP

    Depositor

    Heng Zhao

    Article

    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

    Insert

    cAkt1 (AKT1 Human)
    

    Use

    Lentiviral

    Tags

    HA and IRES-EGFP

    Expression

    Mammalian

    Mutation

    constitutively active (myristoylated version)

    Promoter

    CMV

    Available since

    Oct. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Myc-IQGAP1

    Plasmid

    #30118

    Depositor

    David Sacks

    Article

    Ho et al J Biol Chem. 1999 Jan 1. 274(1):464-70.

    Insert

    IQGAP1 (IQGAP1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSMPP-mCherry-hTRIM21

    Plasmid

    #104972

    Purpose

    Lentiviral Transfer Plasmid with mCherry-tagged human TRIM21

    Depositor

    Leo James

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    Tripartite Motif containing 21 (TRIM21 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p3258 pCMV hUBC9 wt HA

    Plasmid

    #14438

    Depositor

    Peter Howley

    Article

    Yasugi et al Nucleic Acids Res. 1996 Jun 1. 24(11):20

    Insert

    UBC9 (UBE2I Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. L13-Arl13bGFP

    Plasmid

    #40879

    Purpose

    3rd generation transfer vector

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMSCV-IRES-CFP II (pMIC II)

    Plasmid

    #52109

    Purpose

    a bicistronic IRES-CFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDCC6

    Plasmid

    #59985

    Purpose

    Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

    Depositor

    Peter Duchek

    Article

    Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

    Inserts

    Cas9
    U6-2>sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    U6-96Ab and hsp70Bb

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHRm-NLS-dCas9-GFP11x7-NLS

    Plasmid

    #70224

    Purpose

    Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    NLS-dCas9-GFP11x7-NLS
    

    Use

    Tags

    GFP11x7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEJS578_DD-dSpyCas9-mCherry-APEX2

    Plasmid

    #108570

    Purpose

    Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Insert

    dSpyCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    APEX2, Destabilization domains, Flag, and mCherry…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GST-Pin1

    Plasmid

    #19027

    Depositor

    Michael Yaffe

    Article

    Yaffe et al Science. 1997 Dec 12. 278(5345):1957-60.

    Insert

    Pin1 (PIN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMXs-Sox2

    Plasmid

    #13367

    Purpose

    Retroviral expression of mouse Sox2

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    SRY-box containing gene 2 (Sox2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pet30b-7M SrtA

    Plasmid

    #51141

    Purpose

    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity

    Depositor

    Hidde Ploegh

    Insert

    S.aureus SrtA 7M
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, E105K, E108Q, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDESTmycYBX1

    Plasmid

    #19878

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Y box binding protein 1 (YBX1 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV-WT-STAT1

    Plasmid

    #71454

    Purpose

    Lentiviral expression of WT-STAT1

    Depositor

    George Stark

    Article

    Cheon et al Proc Natl Acad Sci U S A. 2009 Jun 9;106

    Insert

    STAT1 (STAT1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

    Plasmid

    #67980

    Purpose

    Cas9 activity reporter with BFP and GFP.

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFC332

    Plasmid

    #87845

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    hph (hygromycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 5xGAL4-TATA-luciferase

    Plasmid

    #46756

    Purpose

    Luciferase reporter gene for factors with GAL4 DNA binding domains

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    minimal TATA

    Available since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMIR-FLAG-MLL-AF9

    Plasmid

    #71444

    Purpose

    Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2

    Depositor

    Daisuke Nakada

    Article

    Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

    Insert

    MLL-AF9 (KMT2A Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Ub-R-GFP

    Plasmid

    #11939

    Depositor

    Nico Dantuma

    Article

    Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

    Insert

    Ub-R-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ubiquitin fused to N-terminus of GFP. Arginine at…

    Promoter

    Available since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBabe puro c-met WT

    Plasmid

    #17493

    Depositor

    Joan Brugge

    Article

    Wrobel et al J Cell Biol. 2004 Apr 26. 165(2):263-73.

    Insert

    c-Met (Met Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Cre Stoplight 2.4

    Plasmid

    #37402

    Purpose

    Cre activity reporter plasmid; Zsgreen is expressed in cells without Cre, mCherry is expressed with Cre

    Depositor

    Thomas Hughes

    Article

    Yang et al Biotechniques. 2001 Nov;31(5):1036, 1038

    Insert

    Cre Stoplight 2.4
    

    Use

    Tags

    ZsGreen1 and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Green cGull

    Plasmid

    #86867

    Purpose

    Green fluorescent probe for cGMP

    Depositor

    Tetsuya Kitaguchi

    Article

    Matsuda et al ACS Sens. 2017 Jan 27;2(1):46-51. doi: 1

    Insert

    Green cGull
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHCMV-AmphoEnv

    Plasmid

    #15799

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    4070A Amphotropic Murine leukemia virus envelope gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

    Plasmid

    #139997

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMK290 (mAID-mClover-Hygro)

    Plasmid

    #72828

    Purpose

    C-terminal tagging with mAID-mClover using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mClover-Hygro
    

    Use

    CRISPR

    Tags

    mAID-mClover

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTRIP-CMV-GFP-FLAG-cGAS

    Plasmid

    #86675

    Purpose

    Expresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

    Depositor

    Nicolas Manel

    Article

    Raab et al Science. 2016 Apr 15;352(6283):359-62. d

    Insert

    GFP-FLAG-cGAS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEGFP-VSP4-E228Q

    Plasmid

    #80351

    Purpose

    Expression of GFP-tagged, dominant negative VPS4 mutant

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    VPS4-E228Q (VPS4A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Flag p21 WT

    Plasmid

    #16240

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

    Insert

    p21 (CDKN1A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge