163,584 results

1. pAAV-U6/sgClta-sfGFP-Clta

   Plasmid

   #200261

   Purpose

   U6 promoter expressing sgClta. Clta homology arms, sfGFP knock-in.

   Depositor

   Justin Eyquem

   Article

   Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

   Insert

   sfGFP flanked by homology arms targeting Clta, additional U6 promoter expressing sgClta
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. 4xM67 pTATA TK-Luc

   Plasmid

   #8688

   Depositor

   Jim Darnell

   Article

   Besser et al Mol Cell Biol 1999 Feb;19(2):1401-9.

   Insert

   4 repeated M67 sites
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 Flag beta-2-adrenergic-receptor

   Plasmid

   #14697

   Depositor

   Robert Lefkowitz

   Article

   Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

   Insert

   B2AR (ADRB2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-IGF1R-V5/HIS

   Plasmid

   #201979

   Purpose

   expression of human IGF1R receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   IGF1R receptor tyrosine kinase (IGF1R Human)
   

   Use

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBZS-M.CviPII (NDel15)

   Plasmid

   #198356

   Purpose

   Express M.CviPII (NDel15) in bacterial cells.

   Depositor

   Takashi Ito

   Article

   Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

   Insert

   M.CviPII
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PLKO.1-Scrambled

   Plasmid

   #136035

   Purpose

   Scrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   None (Scrambled)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Pet28a_Human_H3.1

   Plasmid

   #42631

   Depositor

   Joe Landry

   Insert

   Human H3.1 (H3C1 Human)
   

   Use

   Tags

   Untagged

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 25, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pET-28a_6H-MMLV_RT_D524N-6H

   Plasmid

   #166945

   Purpose

   Bacterial expression of M-MLV reverse transcriptase

   Depositor

   Robert Tjian

   Article

   Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

   Insert

   gag-pol
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   D524N, H8Y

   Promoter

   T7

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGAL4-DBD-MCS

   Plasmid

   #145245

   Purpose

   Backbone vector expressing GAL4 DNA-binding domain followed by MCS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-15F11-HA-mEGFP

    Plasmid

    #129590

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-MHL

    Plasmid

    #26096

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pcDNA3.1 Flag hHMGB1

    Plasmid

    #31609

    Depositor

    Yasuhiko Kawakami

    Article

    Taniguchi et al Proc Natl Acad Sci U S A. 2009 Sep 29. 1

    Insert

    HMGB1 (HMGB1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid

    #118154

    Purpose

    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    EF1a core and U6

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTS118 His14-Avi-28xGS-anti ALFA tag nanobody

    Plasmid

    #199371

    Purpose

    Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    anti-ALFA nanobody
    

    Use

    Tags

    14xHis-Avi

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T5-LacO

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pR26 CAG AsiSI/MluI

    Plasmid

    #74286

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or Mlui

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-SFFV-FLAG-SQSTM1

    Plasmid

    #223715

    Purpose

    Expresses Flag-tagged SQSTM1

    Depositor

    Benjamin Wolozin

    Article

    Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

    Insert

    SQSTM1 (SQSTM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pSIRV-AP-1-mCherry

    Plasmid

    #118095

    Purpose

    Fluorescent reporter for AP-1 activation

    Depositor

    Peter Steinberger

    Article

    Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

    Insert

    mCherry
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    minimal promoter with AP-1 responsive element

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. mEmerald-Sec23A

    Plasmid

    #166893

    Purpose

    mammalian expression of Sec23A tagged with mEmerald

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    SEC23A (SEC23A Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Cas9-GFP_sg_mAMPKa1

    Plasmid

    #79004

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Prkaa1 (Prkaa1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA-HA-eLACCO2.1-NGR

    Plasmid

    #208023

    Purpose

    Biosensor for extracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    eLACCO2.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TK4-EGFP

    Plasmid

    #239047

    Purpose

    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation

    Depositor

    Marius Wernig

    Inserts

    EGFP
    puroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1a

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Cas9-GFP_sg_mAMPKa2

    Plasmid

    #79005

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Prkaa2 (Prkaa2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pUAST-pHuji-GINKO2

    Plasmid

    #177117

    Purpose

    Genetically encoded green fluorescent potassium ion indicator GINKO2 and red fluorescent pH indicator pHuji fusion

    Depositor

    Robert Campbell

    Article

    Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

    Insert

    pHuji-GINKO2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70 promoter

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDestTol2pA2-9xGRE

    Plasmid

    #74736

    Purpose

    GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

    Depositor

    Luisa Dalla Valle

    Article

    Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

    Insert

    EGFP
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    nine GC-responsive elements (9xGRE) from the rat …

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 3xHA-miniTurbo-NLS_pCDNA3

    Plasmid

    #107172

    Purpose

    expresses 3xHA-tagged miniTurbo in the mammalian nucleus

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHR_PGK_antiCD19_synNotch_TetRVP64

    Plasmid

    #79126

    Purpose

    Lentiviral vector for constitutive expression of the anti-CD19 scFv synNotch TetRVP64 Receptor (PGK promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    antiCD19_synNotch_TetRVP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLIB-HIS-TEV-scCAK1

    Plasmid

    #177014

    Purpose

    Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)
    

    Use

    Tags

    Polyhistidine-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBT281

    Plasmid

    #122611

    Purpose

    express evoAPOBEC1-BE4max in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    evoAPOBEC1-BE4max
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E4K H109N H122L D124N R154H A165S P201S F205S

    Promoter

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAS9-mCherry-Frame +0

    Plasmid

    #66939

    Purpose

    CRISPaint frame selector +0

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA frame +0
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pet30b-7M SrtA

    Plasmid

    #51141

    Purpose

    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity

    Depositor

    Hidde Ploegh

    Insert

    S.aureus SrtA 7M
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, E105K, E108Q, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBMN(CMV-copGFP-Puro-SMAR)

    Plasmid

    #80391

    Purpose

    expression copGFP and Puromycine, with an S/MAR element for episomal maintaince

    Depositor

    Magnus Essand

    Article

    Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

    Insert

    copGFP-T2A-Puromycine
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRRL-mU6-mScarletGO-P2A-Blas-PGK-Neo

    Plasmid

    #136904

    Purpose

    Lentivirus for base editing activatable mScarletI expression in mammalian cells. All-in-one vector with sgmScarletGO.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    mScarletGo-p2a-Blas
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pUC19 Giles integrase

    Plasmid

    #170049

    Purpose

    pUC19 with Giles integrase

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Insert

    GIles Integrase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. FSW_jGCaMP8f

    Plasmid

    #197034

    Purpose

    Express GCaMP8f from hSyn1 promoter

    Depositor

    Ronald Hart

    Insert

    GCaMP8f
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMax_GFP

    Plasmid

    #177825

    Purpose

    Host Cell Reactivation (HCR) control GFP plasmid (pMax backbone)

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. spMSP1D1

    Plasmid

    #173482

    Purpose

    Expresses spMSP1D1 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spMSP1D1
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLVX-ATF4 mScarlet NLS

    Plasmid

    #115969

    Purpose

    ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I

    Depositor

    David Andrews

    Article

    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

    Insert

    activating transcription factor 4 (ATF4 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    HA tag, c-myc NLS, and mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCdrA-gfp(ASV)C

    Plasmid

    #111615

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(ASV)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. EGFP-E-Syt2

    Plasmid

    #66831

    Purpose

    Mammalian Expression, Bacterial Expression

    Depositor

    Pietro De Camilli

    Article

    Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

    Insert

    E-Syt2 (ESYT2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AAVS1_Puro_hPGK1_EGFP_Donor

    Plasmid

    #178088

    Purpose

    HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge