We narrowed to 15,371 results for: genome
-
Plasmid#68297PurposeQ157TAG GFP reporterDepositorInsertGFP Q157TAG
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0020
Plasmid#117531PurposeLevel0 Golden Gate part: CDS, SaCas9DepositorInsertSaCas9 (with stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_MP01
Plasmid#194350PurposeCRISPR-Cas9 multiplexing acceptor clone with BpiI cutterDepositorInsertpcoCas9, FAST-Red
UseTagsExpressionPlantMutationPromoterRPS5AAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomSua1
Plasmid#107446PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertSua1
UseTagsV5-HisExpressionYeastMutationSee depositor comments below.PromoterGAL1Available sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet14
Plasmid#107451PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet14 (met14 Fission Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet1
Plasmid#107444PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet1
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBRC1
Plasmid#183064PurposeBase editing plasmid for Pseudomonas chlororaphisDepositorInserteSpCas9ppD10A
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas9-J23109
Plasmid#113150PurposePlasmid constitutively expressing Cas9 proteinDepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4092709
Plasmid#153859PurposeBacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTRDepositorInsertfragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionBacterialMutationNearly native encoding;handful SNPs as watermarks…PromoterPromoter | t7 consensus from NEB, G is +1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYES2.1-cerMET6
Plasmid#107438PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET6 (MET6 Budding Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR1054
Plasmid#71822PurposeExpress Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBA789
Plasmid#186244Purposesoc-F HR Donor Vector (T4 phage editing)DepositorInsertBBR1 backbone + T4 phage homology
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC-PTP-NEO
Plasmid#51710Purposecontains a C-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.DepositorTypeEmpty backboneUseT. brucei genome integration vectorsTagsPTP (ProtC-TEV-ProtA)ExpressionMutationPromoterAvailable sinceMarch 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
-
PX458_CREB1_2
Plasmid#64940PurposeExpresses gRNA against human CREB1 along with Cas9 with 2A GFPDepositorInsertgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-CaARG4
Plasmid#162463PurposeDNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaARG4DepositorInsert3FLAG-Mnase
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
mTALEAD 16S rRNA-1139T10C
Plasmid#210838PurposemTALEAD expression vectorDepositorInsertFor specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
p221z-AtMIR390a
Plasmid#118388PurposeEntry clone containing AtMIR390a backbone (split by ccdB gene). Used to make artificial microRNA construct. For use in plants and compatible with the MultiSite Gateway system.DepositorInsertAtMIR390a backbone
UseRNAiTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK1200
Plasmid#84219PurposeLentiviral expression of shRNAs, compatible with double-shRNA expression, EF1alpha promoter, mCherryDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBA790
Plasmid#186245Purposednap-C HR Donor Vector (T4 phage editing)DepositorInsertBBR1 backbone + T4 phage homology
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET16
Plasmid#107442PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET16 (MET16 Budding Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFGL1258
Plasmid#116898PurposeTetOFF:GFP:CenpA (centromere marker)DepositorInsertsCenpA promoter region
CenpA
UseFungal expression (in magnaporthe oryzae)TagsExpressionMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEVA23-GG
Plasmid#208013Purposefor cloning homology arms, with GFP as counter selection markerDepositorInsertmsfGFP
UseTagsExpressionMutationPromoter14GAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR1003
Plasmid#71821PurposeExpress Streptococcus pyogenes dCas9 (D10A, H840A)DepositorInsertT7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
peft-3::cas-9::tbb-2 3'UTR
Plasmid#48960Purposethis plasmid contains the codon optimized gene encoding Cas9 for C. elegans.DepositorInsertcas9
UseCRISPRTagsExpressionMutationPromotereef-1A.1 (eft-3)Available sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEM-sgControl_dual-MTS
Plasmid#162765PurposeExpressing sgControl_dual-MTSDepositorInsertsgControl_dual-MTS
UseTagsExpressionMammalianMutationPromoterU6Available sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221TrxA-AID-T7RNAP
Plasmid#167976PurposeExpresses fusion protein TrxA-AID-T7RNAP in E. coliDepositorInsertTrxA-AID-T7RNAP
UseTagsT7RNAP and TrxAExpressionBacterialMutationPromoterPtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOsU3-esgRNA
Plasmid#115629Purposeexpression esgRNA in rice protoplastsDepositorInsertOsU3p-esgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET10
Plasmid#107440PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET10 (MET10 Budding Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pN-PURO-PTP
Plasmid#51711Purposecontains an N-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.DepositorTypeEmpty backboneUseT. brucei genome integration vectorsTagsPTP (ProtC-TEV-ProtA)ExpressionMutationPromoterAvailable sinceMarch 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0013
Plasmid#117521PurposeLevel0 Golden Gate part: CDS, SpCas9-pDepositorInsertSpCas9-p (with stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX458_GABPA_2
Plasmid#64255PurposeExpresses gRNA against human GABPA along with Cas9 with 2A GFPDepositorInsertgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNHP
Plasmid#22500DepositorInsertgag, pol, tat, rev
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
p3s-Cas9-HF1
Plasmid#104173PurposeCas9-HF1 mammalian expressionDepositorInsertCas9-HF1
UseCRISPRTagsHAExpressionMammalianMutationN497A, R661A, Q695A, Q926APromoterCMVAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLSLZ.D.R
Plasmid#51510PurposeSingle component LSL vector. Contains 2x35S:Zif268:FokI followed by the us:NPTII template. This sequence is between the LIR and SIR.DepositorInsertsZif268:FokI
us:NPTII repair template
UsePlant t-dna plasmidTagsNLSExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
lentiguide-puro-wt-ltr
Plasmid#182635PurposeExpresses guide RNA, contains intact LTRDepositorInsertS. pyogenes sgRNA cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenticrispr-wt-ltr-puro
Plasmid#173428PurposeExpresses Cas9 and guide RNA, contains intact LTRDepositorInsertS. pyogenes sgRNA cassette
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3s-eCas9-1.1
Plasmid#104172PurposeeCas9-1.1 mammalian expressionDepositorInserteCas9-1.1
UseCRISPRTagsHAExpressionMammalianMutationK848A, K1003A, R1060APromoterCMVAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
AP582-2
Plasmid#70049PurposeExpresses Ollas::mCherry::linker::H2B::V5 in bacteriaDepositorInsertmCherry::linker::H2B
UseTagsOllas and V5ExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
B1979
Plasmid#59181PurposeExpresses optimized blasticidin S resistance gene (BSD) for C. elegansDepositorInsertBlasticidin S resistance
UseTagsExpressionWormMutationPromoterrpl-28Available sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
PX458_GABPA_1
Plasmid#64254PurposeExpresses gRNA against human GABPA along with Cas9 with 2A GFPDepositorInsertgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ147
Plasmid#69299PurposeGolden Gate recipient and Gateway entry vector; assembly of 7 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRF-AmeR
Plasmid#49356DepositorInsertAmeR repressor
UseSynthetic BiologyTagsExpressionBacterialMutationD120F, R121Q, G123H, Y124T, E125G, M126F, W127YSPromoterAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR1055
Plasmid#71823PurposeExpress Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
CXXC1
Plasmid#32045PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCXXC1 (CXXC1 Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationCXXC domain (amino acids residues 161-222)PromoterAvailable sinceJan. 3, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
BSP608
Plasmid#122253Purposeexpression vector for mNeptune under EFT3 promoterDepositorInsertmNeptune
UseTagsExpressionWormMutationPromoterEFT3Available sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Crispr-V2-HMID.v1
Plasmid#103062PurposeLentiCrisprV2 with guide targeting V1-V5 GESTALT barcodesDepositorInsertCas9 plus expressed GESTALT V1-V5 guide
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-C3orf17-flag
Plasmid#70646PurposeExpresses C3orf17 in mammalian cells with FLAG-tag epitope.DepositorInsertC3orf17 (NEPRO Human)
UseRetroviralTags3X FLAGExpressionMammalianMutationPromoterAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only