166,201 results

1. pRK5-HA-Ubiquitin-K63

   Plasmid

   #17606

   Purpose

   Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K63 only, other lysines mutated to arginines. Enh…

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

   Plasmid

   #199390

   Purpose

   Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   anti-ALFA nanobody
   

   Tags

   14xHis-Avi-SUMOEu

   Expression

   Bacterial

   Promoter

   T5-LacO

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. LMAN2-bio-His

   Plasmid

   #51923

   Purpose

   Expresses full-length Vesicular integral-membrane protein VIP36 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   LMAN2 (LMAN2 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO-GAlphaQ-RLuc8

   Plasmid

   #140982

   Purpose

   Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphaQ-RLuc8 (GNAQ Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-Rab7A

   Plasmid

   #61803

   Purpose

   Human Rab7A fluorescently tagged with GFP on the N-terminus for mammalian expression

   Depositor

   Gia Voeltz

   Article

   Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

   Insert

   Rab7A (RAB7A Human)
   

   Tags

   2x HA and GFP

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-HA-Ubiquitin-K48

   Plasmid

   #17605

   Purpose

   Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K48 only, other lysines mutated to arginines. Enh…

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. GW1-pHRed

   Plasmid

   #31473

   Purpose

   genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima

   Depositor

   Gary Yellen

   Article

   Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

   Insert

   pHRed
   

   Expression

   Mammalian

   Available Since

   July 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. p16 nlsGPS2

   Plasmid

   #228093

   Purpose

   Expression plasmid for genetically-encoded FRET biosensor for Gibberellin (GA)

   Depositor

   Alexander Jones

   Article

   Griffiths et al Plant Cell. 2024 Jul 23:koae198. doi: 10

   Insert

   nlsGPS2
   

   Tags

   6xHis-T7 XpressTag and cMyc

   Expression

   Plant

   Promoter

   p16

   Available Since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. tetO.Sox9.Puro

   Plasmid

   #117269

   Purpose

   Expresses Sox9 and puromycin resistance gene under control of tetO sequence.

   Depositor

   Henrik Ahlenius

   Article

   Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

   Insert

   Sox9 (Sox9 Mouse)
   

   Use

   Lentiviral

   Tags

   T2A and puromycin resistant cassette (C terminal …

   Expression

   Mammalian

   Promoter

   tet-on

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

    Plasmid

    #67980

    Purpose

    Cas9 activity reporter with BFP and GFP.

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
    

    Use

    Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIM1773-PRHIII-sfGFP-pNW33N

    Plasmid

    #52217

    Purpose

    Constitutive sfGFP expression plasmid for use in Geobacillus and E. coli

    Depositor

    Ichiro Matsumura

    Article

    Blanchard et al Appl Microbiol Biotechnol. 2014 May 2.

    Insert

    PRHIII-sfGFP
    

    Expression

    Bacterial

    Promoter

    PRHIII

    Available Since

    May 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTargetF

    Plasmid

    #62226

    Purpose

    Constitutive expression of sgRNA without donor editing template DNA

    Depositor

    Sheng Yang

    Article

    Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

    Insert

    sgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pij23119

    Available Since

    March 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCI pHluorin Syt12

    Plasmid

    #184513

    Purpose

    Mammalian expression of Synaptotagmin 12 with pH-sensitive fluorescent protein

    Depositor

    Edwin Chapman

    Article

    Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

    Insert

    Synaptotagmin 12 (Syt12 Rat)
    

    Tags

    Super Ecliptic pHluorin

    Expression

    Mammalian

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT-CA-GFP-IRES-mLumin

    Plasmid

    #32749

    Depositor

    Jeanne Hardy

    Article

    Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-8

    Inserts

    CA-GFP
    mLumin
    

    Expression

    Mammalian

    Mutation

    S158A and S65T

    Promoter

    CMV

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. miniCMV-(mNeonGreen)4-tDeg

    Plasmid

    #129402

    Purpose

    (mNeonGreen)4-tDeg fluorogenic protein

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    (mNeonGreen)-tDeg
    

    Expression

    Mammalian

    Promoter

    miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBabe-Kras Wt

    Plasmid

    #75282

    Purpose

    Expresses human Kras wild-type

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    None. Wild-Type

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKSE401

    Plasmid

    #62202

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    35S and AtU6-26p

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUK21

    Plasmid

    #49788

    Purpose

    E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

    Depositor

    Joachim Messing

    Article

    Vieira et al Gene. 1991 Apr;100:189-94.

    Type

    Empty backbone

    Use

    Cloning vector

    Promoter

    lacZ

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCF1148

    Plasmid

    #222330

    Purpose

    Fluorescent reporter plasmid for Veillonella

    Depositor

    J Christopher Fenno

    Article

    Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

    Insert

    bs2 gene with Veillonella parvula SKV38 mdh promoter
    

    Use

    E. coli-veillonella shuttle plasmid

    Expression

    Bacterial

    Promoter

    Veillonella parvula SKV38 mdh promoter

    Available Since

    Oct. 31, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. PPRE X3-TK-luc

    Plasmid

    #1015

    Purpose

    PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

    Depositor

    Bruce Spiegelman

    Article

    Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

    Insert

    PPRE
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Nov. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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21. pPSU20-40-60-80

    Plasmid

    #173839

    Purpose

    Coexpresses 20, 40, 60 and 80 kD Penn State ladder proteins

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPABPACCBPv3
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. Flag p21 WT

    Plasmid

    #16240

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

    Insert

    p21 (CDKN1A Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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23. pBabe-GFP-IKBalpha-wt

    Plasmid

    #15263

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKB alpha (NFKBIA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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24. pNP3386 [For use in genome insertion and off-target assessment]

    Plasmid

    #247258

    Purpose

    Example plasmid for bridge recombinase mediated insertion and off-target assessment with puromycin selection — must clone in UMI

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    ISCro4 Recognition Site-UMI-Ef1a-PuroR-P2A-mCherry (Plasmid for Insertion Site Mapping [Example, Clone in Sequence + UMI])
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. pDONR221_SLC37A4

    Plasmid

    #131914

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC37A4 (SLC37A4 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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26. pX601-GFP

    Plasmid

    #84040

    Purpose

    Staphylococcus aureus (SaCas9) conjugated with GFP

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    NLS and T2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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27. HSD11B1

    Plasmid

    #39155

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    HSD11B1 (HSD11B1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    T220I; codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

28. ppyCAG_RNaseH1_WKKD

    Plasmid

    #111905

    Purpose

    Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids

    Depositor

    Xiang-Dong Fu

    Article

    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

    Insert

    RNASEH1 (RNASEH1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…

    Available Since

    July 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET29-bdSUMO-Anti-ALFA nanobody

    Plasmid

    #212311

    Purpose

    Expression of anti-ALFA nanobody

    Depositor

    Arthur Laganowsky

    Article

    Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

    Insert

    anti-ALFA nanobody
    

    Tags

    bdSUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ABE8e

    Plasmid

    #138489

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-nSpCas9
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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31. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid

    #185912

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpRY-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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32. pX330 p53

    Plasmid

    #59910

    Purpose

    pX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    sgRNA targeting p53 (Trp53 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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33. pAAV-CAG-EGFP barcode-5-SV40 polyA

    Plasmid

    #190869

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode5
    

    Use

    AAV

    Mutation

    WT

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CMV-mito-R-GECO1

    Plasmid

    #46021

    Purpose

    Red intensiometric genetically encoded Ca2+-indicators for optical imaging

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Neurosci. 2013 Mar 19.

    Insert

    R-GECO1
    

    Tags

    a duplex of the mitochondrial targeting signal of…

    Expression

    Mammalian

    Mutation

    Substitutions relative to the mApple-derived anal…

    Promoter

    CMV

    Available Since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCF1135

    Plasmid

    #222106

    Purpose

    E. coli - Veillonella shuttle plasmid with single selectable marker

    Depositor

    J Christopher Fenno

    Article

    Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

    Type

    Empty backbone

    Use

    E. coli-veillonella shuttle plasmid

    Expression

    Bacterial

    Available Since

    Oct. 31, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid

    #213026

    Purpose

    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1-mCherry

    Plasmid

    #128744

    Purpose

    Expresses mCherry in mammalian cells

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pXR003: CasRx gRNA cloning backbone

    Plasmid

    #109053

    Purpose

    hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)

    Plasmid

    #153005

    Purpose

    Expresses high-affinity split-TurboID C-terminal fragment in mammalian cells

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID-high affinity (C) (G79)
    

    Use

    Lentiviral

    Tags

    FRB, HA, and HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV-PE6b

    Plasmid

    #207852

    Purpose

    Mammalian expression of PE6b prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6b
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge