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We narrowed to 7,491 results for: RAP

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Showing: 3151 - 3200 of 7491 results

  1. pPT-TAR C1

    Plasmid
    #141246
    Purpose
    Transformation Associated Recombination (TAR) cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1
    Depositor
    Bogumil Karas
    Article
    Cochrane et al Algal Research, 2020. 50, 102014
    Insert
    Mitochondrial Genome of Phaeodactylum tricornutum
    Use
    Synthetic Biology; Diatom expression
    Expression
    Bacterial and Yeast
    Mutation
    Minor additional mutations described in manuscript
    Available Since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    141246-plasmid-map-sequence-id-367010

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  10. pRXG

    Plasmid
    #113643
    Purpose
    This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    mRFP1-linker-sfGFP
    Use
    Synthetic Biology
    Available Since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    113643-plasmid-map-sequence-id-217116

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  17. FRB_SCF2_LD6_CTEV

    Plasmid
    #58879
    Purpose
    MESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV protease
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58879-plasmid-map-sequence-id-94240

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  19. FKBP_SCF2_LD6_NTEV

    Plasmid
    #58878
    Purpose
    MESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV protease
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58878-plasmid-map-sequence-id-94239

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  23. pACT5FVMF(f)

    Plasmid
    #110549
    Purpose
    Control plasmid for titering FV by qPCR to determine the genome-containing particle number. This qPCR protocol is described in Hendrie et al., 2008
    Depositor
    David Russell
    Article
    Hendrie et al Mol Ther. 2008 Mar;16(3):534-40. doi: 10
    Insert
    EGFP, FV LTRs
    Use
    Qpcr control for titering foamy virus
    Promoter
    promoter: MLV LTR
    Available Since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110549-plasmid-map-sequence-id-212295

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  24. pcDNA3-FRB-VMAc-CGFP

    Plasmid
    #70226
    Purpose
    Expresses C-terminal portion of GFP along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    eGFP
    Tags
    FKBP-rapamycin binding (FRB) domain of the mammal…
    Expression
    Mammalian
    Mutation
    Changed Ile 129 to Cys, Deleted amino acids 1-128
    Promoter
    CMV
    Available Since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  26. pThermeleon

    Plasmid
    #86120
    Purpose
    Bandpass temperature-gated expression of mWasabi and mCherry reporters
    Depositor
    Mikhail Shapiro
    Article
    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi
    Inserts
    TlpA
    mWasabi
    TcI38
    mCherry
    cI
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    A67T, M1V, L65S, K68R, F115L, D126G, D188G and D3…
    Promoter
    pR/pL, pR/pL, pLacI, and pTlpA
    Available Since
    March 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86120-plasmid-map-sequence-id-173201

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  32. pEGFP-rNFM

    Plasmid
    #32907
    Purpose
    Mammalian expression construct driving the expression of a fluorescent fusion protein consisting of EGFP linked to the N-terminus of rat neurofilament protein M
    Depositor
    Anthony Brown
    Article
    Wang et al Nat Cell Biol. 2000 Mar;2(3):137-41.
    Insert
    EGFP-rat NFM fusion (Nefm Rat)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32907-plasmid-map-sequence-id-38889

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  34. pRF0G-IodoY

    Plasmid
    #113645
    Purpose
    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    IodoYRS and tRNA gene
    Use
    Synthetic Biology
    Available Since
    July 27, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    113645-plasmid-map-sequence-id-217127

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  44. pGL2399

    Plasmid
    #137051
    Purpose
    Expression of rapamycin-inducible dimerisation of split Cre from ribosomal locus in Leishmania parasite; pRIB-DiCre
    Depositor
    Jeremy Mottram
    Article
    Duncan et al Methods Mol Biol. 2019;1971:211-224. doi
    Inserts
    Split Cre Recombinase Rapamycin Inducible Dimerisation
    Blasticidin-S Deaminase
    Use
    Cre/Lox; Leishmania ribosomal locus expression
    Promoter
    For integration into the 18S ribosomal locus
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    137051-plasmid-map-sequence-id-275797

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Showing: 3151 - 3200 of 7491 results