4,136 results

1. pSLQ1661-sgMUC4-E3(F+E)

   Plasmid

   #51025

   Purpose

   Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3

   Depositor

   Stanley Qi, Bo Huang

   Article

   Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

   Insert

   optimized sgRNA (MUC4 Mouse, Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-GCaMP5G

   Plasmid

   #31788

   Purpose

   a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

   Depositor

   GENIE Project, Douglas Kim, Loren Looger

   Article

   Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

   Insert

   GCaMP5G
   

   Use

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLOX-gfp-iresTK

   Plasmid

   #12243

   Depositor

   Didier Trono

   Article

   Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

   Insert

   GFP, HSV1-TK
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   LoxP in 3' LTR.

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMVTNT PINK1 N-myc

   Plasmid

   #13313

   Depositor

   Mark Cookson

   Article

   Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

   Insert

   PINK1 (PINK1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. hSERT pcDNA3

   Plasmid

   #15483

   Depositor

   Randy Blakely

   Article

   Ramamoorthy et al Proc Natl Acad Sci U S A. 1993 Mar 15. 9

   Insert

   hSERT (SLC6A4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-DIO eNpHR 3.0-EYFP

   Plasmid

   #26966

   Purpose

   AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition

   Depositor

   Karl Deisseroth

   Article

   Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   eNpHR 3.0
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   ER export at 3' end of gene. Trafficking sig…

   Promoter

   EF1alpha

   Available since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCGN-SRF

   Plasmid

   #11977

   Purpose

   For mammalian expression of human SRF with an HA tag

   Depositor

   Ron Prywes

   Article

   Johansen et al Mol Cell Biol. 1993 Aug . 13(8):4640-7.

   Insert

   SRF (SRF Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Alpha 5 integrin-GFP

   Plasmid

   #15238

   Depositor

   Rick Horwitz

   Article

   Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

   Insert

   alpha 5 integrin (ITGA5 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-PGC1

   Plasmid

   #4

   Depositor

   Bruce Spiegelman

   Article

   Puigserver et al Cell 1998 Mar 20;92(6):829-39.

   Insert

   PGC-1 alpha (Ppargc1a Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3 Tat HA (P#29)

    Plasmid

    #14654

    Depositor

    Matija Peterlin

    Article

    Cujec et al Genes Dev. 1997 Oct 15. 11(20):2645-57.

    Insert

    Tat (tat HIV)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pQFlag-USP7 WT puroR

    Plasmid

    #46751

    Purpose

    Retroviral vector that expresses wild type Flag-tagged human USP7

    Depositor

    Gordon Peters, Goedele Maertens

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP7 (USP7 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-FoxO1-Clover

    Plasmid

    #67759

    Purpose

    Fluorescent fusion protein for tracking Akt signaling

    Depositor

    Peter Rotwein

    Article

    Gross et al J Cell Sci. 2015 Jun 3. pii: jcs.168773.

    Insert

    Fox01 (Foxo1 Mouse)
    

    Use

    Lentiviral

    Tags

    Clover

    Expression

    Mutation

    Changed Serine 209 to Alanine, changed Histidine …

    Promoter

    EF1a

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EGFP-hAgo2

    Plasmid

    #21981

    Depositor

    Phil Sharp

    Article

    Leung et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pmScarlet-H_C1

    Plasmid

    #85043

    Purpose

    Mammalian expression of mScarlet-H

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    mScarlet-H
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST pcDNA5-FRT/TO-eGFP-Rif1

    Plasmid

    #52506

    Purpose

    mammalian expression vector of Rif1 siRNA resistant

    Depositor

    Daniel Durocher

    Article

    Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

    Insert

    RIF1 (RIF1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    siRNA resistant sequence: ATAGTAC

    Promoter

    Available since

    June 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. TetO-FUW-oct4

    Plasmid

    #20323

    Depositor

    Rudolf Jaenisch

    Article

    Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

    Insert

    oct4 (Pou5f1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Stat3-C Flag pRc/CMV

    Plasmid

    #8722

    Depositor

    Jim Darnell

    Article

    Bromberg et al Cell 1999 Aug 6;98(3):295-303.

    Insert

    Stat3-C (Stat3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    A662C and N664C. Residues changed to Cys renders…

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HDAC8 Flag

    Plasmid

    #13825

    Depositor

    Eric Verdin

    Article

    Waltregny et al FASEB J. 2005 Jun . 19(8):966-8.

    Insert

    HDAC8 (HDAC8 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Cas12f-GE ver4.1

    Plasmid

    #176544

    Purpose

    Expresses Cas12f-GE in mammalian cells

    Depositor

    Yong-Sam Kim

    Article

    Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

    Inserts

    Cas12f-GE ver4.1
    Cas12f-GE ver4.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken β-actin

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCFJ104 - Pmyo-3::mCherry::unc-54

    Plasmid

    #19328

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pmyo-3::mCherry
    

    Use

    Tags

    mCherry

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pU6-sgGFP-NT1

    Plasmid

    #46914

    Purpose

    Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1)

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    sgGFP-NT1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CMV-R-GECO1.2

    Plasmid

    #45494

    Purpose

    Red intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Neurosci. 2013 Mar 19.

    Insert

    R-GECO1.2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

    Promoter

    CMV

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGFP-EB1

    Plasmid

    #17234

    Depositor

    Lynne Cassimeris

    Article

    Piehl et al Mol Biol Cell. 2003 Mar . 14(3):916-25.

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-EGFP-Cdc42-Q61L

    Plasmid

    #12986

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Cdc42 constitutively active (CDC42 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q61L Constitutively active

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHAGE EF1α dCas9-VP64

    Plasmid

    #50918

    Purpose

    Constitutive dCas9-VP64 lentiviral expression vector

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and VP64

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1alpha

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRSETa mEos2

    Plasmid

    #20341

    Purpose

    bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)

    Depositor

    Loren Looger

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    mEos-2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET MBP His6 LIC cloning vector (2Cc-T)

    Plasmid

    #37237

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMSCV-IRES-Blue FP

    Plasmid

    #52115

    Purpose

    a bicistronic IRES-Blue FP containing retroviral vector

    Depositor

    Dario Vignali

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-Blue FP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pC1-HyPer-Red-C199S

    Plasmid

    #48252

    Purpose

    Hydrogen peroxide insensitive version of HyPerRed to be used as a pH control in HyPer experiments.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    HYPER-RED-C199S
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-32a(+)-NRG1B1

    Plasmid

    #120287

    Purpose

    Produce human recombinant NRG1 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    NRG1-beta 1
    

    Use

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Myc-TEAD4

    Plasmid

    #24638

    Depositor

    Kunliang Guan

    Article

    Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

    Insert

    TEAD-4 (TEAD4 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA

    Plasmid

    #20299

    Purpose

    Cre-activated AAV expression of mCherry

    Depositor

    Karl Deisseroth

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pENTR4-V5 (w71-3)

    Plasmid

    #17425

    Purpose

    Gateway entry vector for N-term V5 tag, frame A

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV-PTEN

    Plasmid

    #28298

    Depositor

    Maria-Magdalena Georgescu

    Article

    Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFRT-TODestFLAGHAhFMRPiso1

    Plasmid

    #48690

    Purpose

    Expresses hFMRP iso 1

    Depositor

    Thomas Tuschl

    Article

    Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

    Insert

    FlagHA FMR1 Iso 1 (FMR1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    May 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSLIK-IDH1-R132H-FLAG

    Plasmid

    #66803

    Purpose

    Tet-inducible expression of mutant IDH1 in mammalian cells, 3rd generation lentiviral vector

    Depositor

    Christian Metallo

    Article

    Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R132H

    Promoter

    CMV

    Available since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAC154-dual-dCas9VP160-sgExpression

    Plasmid

    #48240

    Purpose

    Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    HA-Tag, HA-tag, and VP160

    Expression

    Mammalian

    Mutation

    D10A H840A

    Promoter

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLL7.0: Venus-iLID-Mito (From ActA)

    Plasmid

    #60413

    Purpose

    Mammalian Expression of Venus-iLID-Mito

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    Venus-iLID-Mito
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTAT-HA

    Plasmid

    #35612

    Depositor

    Steven Dowdy

    Article

    Nagahara et al Nat Med. 1998 Dec;4(12):1449-52.

    Type

    Empty backbone

    Use

    Tags

    6xHIS, HA, and TAT

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCuo CA Rac1

    Plasmid

    #84643

    Purpose

    constitutively active Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.

    Depositor

    Sanjay Kumar

    Article

    MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Constitutively Active, Q61L

    Promoter

    cumate-inducible promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge