161,273 results

1. pPBbsr-mKO-MK2

   Plasmid

   #115492

   Purpose

   Expression vector for the p38 activity reporter mKO-MK2 in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

   Insert

   mKO-MK2 (MAPKAPK2 Human)
   

   Use

   Tags

   monomeric Kusabira Orange (mKO)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-His3MX6

   Plasmid

   #41596

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   His3
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF3550

   Plasmid

   #145026

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFRT_TO_eGFP_TIA1

   Plasmid

   #106094

   Purpose

   gene expression of EGFP-TIA1

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

   Insert

   TIA1 (TIA1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3 Control Vector

   Plasmid

   #212937

   Purpose

   Control vector with constitutive firefly luciferase expression

   Depositor

   Debrya Groskreutz

   Insert

   luc+
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   WT

   Promoter

   SV40

   Available since

   Jan. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. Lenti-CamKII-PSD95.FingR-eGFP-CCR5TC

   Plasmid

   #126218

   Purpose

   Global labeling of excitatory synapses (green fluorescence)

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   PSD95.FingR-eGFP-CCR5TC
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CamKIIa

   Available since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-C1 Lifeact-EGFP

   Plasmid

   #58470

   Purpose

   Expresses a cytoplasmic actin filament reporter, the peptide Lifeact, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Bioarchitecture. 2015 Aug 28:1-14.

   Insert

   Lifeact
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1-Puro CAG J591-scFV T-CAR

   Plasmid

   #171961

   Purpose

   Donor plasmid for J591 T-CAR expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Harris et al J Immunol Regen Med. 2023 May;20:100074.

   Insert

   Synthetic J591-scFV CAR
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pErCas12a EZ Clone

   Plasmid

   #132641

   Purpose

   Expresses ErCas12a and an sgRNA to target a region of interest

   Depositor

   Stephen Ekker

   Article

   Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

   Insert

   ErCas12a
   

   Use

   AAV and CRISPR

   Tags

   3x HA and SV40 NLS

   Expression

   Mammalian

   Mutation

   Q926R (Please see depositor comments) and BsaI si…

   Promoter

   CMV Promoter

   Available since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. ER-J-TAG (pGS730)

    Plasmid

    #160038

    Purpose

    pLV-A4-UCOE-EF1-3xHA-sfGFP-JPH-TMD-IRES-BLAS-2A-SNAP-WPRE-A2

    Depositor

    David Sabatini

    Insert

    3xHA-sfGFP-JPH-TMD
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP hsp70

    Plasmid

    #15215

    Depositor

    Lois Greene

    Article

    Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

    Insert

    hsp70 (HSPA1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-EGFP-2xFYVE

    Plasmid

    #136996

    Purpose

    Lentiviral EGFP lipid sensor for PI(3)P

    Depositor

    Ken-Ichi Takemaru

    Insert

    EGFP-2xFYVE
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. V5-GFP-APEX2-NES_pLX208

    Plasmid

    #202018

    Purpose

    expresses GFP-APEX2 in the mammalian cytosol, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    V5-GFP-APEX2-NES
    

    Use

    Lentiviral

    Tags

    GFP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMJ806

    Plasmid

    #39312

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. mEmerald-Calreticulin-N-16

    Plasmid

    #54023

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Calreticulin (CALR Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCS2+HyPer7-NLS

    Plasmid

    #136468

    Purpose

    Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Triple nuclear localization signal of SV40 (simia…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV3flag8HOIP

    Plasmid

    #50015

    Purpose

    Mammalian expression of human HOIP (RNF31) with a c-terminal 3xFLAG tag, and hygromycin selection.

    Depositor

    Martin Dorf

    Article

    Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

    Insert

    ring finger protein 31 (RNF31 Human)
    

    Use

    Tags

    3 x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-MAP1LC3B

    Plasmid

    #137757

    Purpose

    Expression of LC3B

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. p101_CRISPRai_PiggyBac

    Plasmid

    #213777

    Purpose

    Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Inserts

    VPR-dSaCas9
    dSpCas9-KRAB-BFP
    zeocin
    Tet-On transactivator
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1a (EF-1-alpha intron a) and Tet_On_TRE

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. RG6

    Plasmid

    #80167

    Purpose

    fluorescent protein splicing reporter

    Depositor

    Thomas Cooper

    Article

    Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

    Insert

    dsRed and GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLV UBC hTMEM115-3C-3xHA-P2A-eGFP

    Plasmid

    #214996

    Purpose

    Stably express the GolgiTag (GolgiIP handle) in mammalian cells (human ORF). eGFP for cell sorting desired expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    TMEM115 (TMEM115 Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP-N1-MITF-M

    Plasmid

    #38131

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    MITF-M (MITF Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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23. mCherry-NLS-mCherry

    Plasmid

    #231890

    Purpose

    mCherry-tagged nuclear rupture marker

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    mCherry-SV40 NLS-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Lamp1-RFP

    Plasmid

    #1817

    Purpose

    Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.

    Depositor

    Walther Mothes

    Article

    Sherer et al Traffic 2003 Nov;4(11):785-801.

    Insert

    lysosome associated membrane protein 1 (Lamp1 Rat)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    D50E (not important for function of plasmid)

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pmCherry-NLS

    Plasmid

    #39319

    Depositor

    Martin Offterdinger

    Article

    Micutkova et al Int J Cancer. 2012 Apr 1;130(7):1544-57.

    Insert

    mCherry-3xNLS
    

    Use

    Tags

    3xSV40LT NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMOS005: superecliptic pHluorin (cytosolic)

    Plasmid

    #163049

    Purpose

    superecliptic pHluorin (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    superecliptic pHluorin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. eeBxb1

    Plasmid

    #222339

    Purpose

    Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved and engineered Bxb1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pT2/shp53/GFP4

    Plasmid

    #20208

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    shp53/GFP4
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p18sheLL

    Plasmid

    #37321

    Depositor

    John Schiller

    Article

    Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

    Insert

    HPV18L1+L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PB_TetON_mCherry_P2AT2A_GFP-nb-KS

    Plasmid

    #238240

    Purpose

    For integration of mCherry_P2AT2A_GFP-nb-KS

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb-KS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge