4,100 results

1. pENTR5'_ubi

   Plasmid

   #27320

   Depositor

   Leonard Zon

   Article

   Mosimann et al Development. 2011 Jan . 138(1):169-77.

   Insert

   zebrafish ubiquitin promoter (ubb Zebrafish)
   

   Use

   Multisite gateway 5' entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV(CAT)T7-SB100

   Plasmid

   #34879

   Depositor

   Zsuzsanna Izsvak

   Article

   Mates et al Nat Genet. 2009 Jun;41(6):753-61. Epub 2

   Insert

   SB100X transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. rtTA-N144

   Plasmid

   #66810

   Purpose

   2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistance

   Depositor

   Andrew Yoo

   Article

   Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

   Insert

   Reverse tetracycline-controlled transactivator
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1a

   Available since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-FLEX-DTR-GFP

   Plasmid

   #124364

   Purpose

   conditional expression of a diphtheria toxin receptor (DTR)–GFP fusion protein

   Depositor

   Eiman Azim, Thomas Jessell

   Article

   Azim et al Nature. 2014 Apr 17;508(7496):357-63. do

   Has Service

   AAV2

   Insert

   DTR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Chicken B-actin

   Available since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSIRV-NF-kB-eGFP

   Plasmid

   #118093

   Purpose

   Fluorescent reporter for NF-kB activity

   Depositor

   Peter Steinberger

   Article

   Jutz et al Oncotarget. 2017 May 4;8(39):64892-64906

   Insert

   eGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   minimal promoter with NF-kB responsive element

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHM6-alphasynuclein-A53T

   Plasmid

   #40825

   Depositor

   David Rubinsztein

   Article

   Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

   Insert

   SNCA (SNCA Human)
   

   Use

   Tags

   6x His and HA

   Expression

   Mammalian

   Mutation

   A53T

   Promoter

   CMV

   Available since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. CD63-pEGFP C2

   Plasmid

   #62964

   Purpose

   full length human CD63 cloned into pEGFP C2

   Depositor

   Paul Luzio

   Insert

   CD63 molecule (CD63 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   CD63 has 1 silent mutation compared to the coding…

   Promoter

   CMV

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-EGFP

   Plasmid

   #13031

   Purpose

   Mammalian expression vector for cloning your gene fused to EGFP

   Depositor

   Doug Golenbock

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. Fuw-dCas9-Tet1CD-P2A-BFP

   Plasmid

   #108245

   Purpose

   Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

   Insert

   dCas9-Tet1CD-HA-P2A-BFP
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   dCas9-D10A, dCas9-H840A

   Promoter

   Ubc

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pgRNA-bacteria

    Plasmid

    #44251

    Purpose

    Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    guide RNA (bacteria)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available since

    April 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-Neo-Bam p53 R175H

    Plasmid

    #16436

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R175H (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R175H

    Promoter

    CMV

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNIC28-Bsa4

    Plasmid

    #26103

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Jun 10. ():.

    Type

    Empty backbone

    Use

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-LucOS

    Plasmid

    #22777

    Depositor

    Tyler Jacks

    Insert

    OVA-SIY
    

    Use

    Cre/Lox and Lentiviral

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-Neo-Bam p53 R248W

    Plasmid

    #16437

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R248W (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R248W

    Promoter

    Available since

    Aug. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS598 EF1alpha-EGFPcre

    Plasmid

    #11923

    Purpose

    Expresses EGFP-Cre fusion under the control of the EF1alpha promoter; EGFP is human codon-optimized.

    Depositor

    Brian Sauer

    Article

    Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

    Insert

    EGFP-cre (cre bacteriophage P1)
    

    Use

    Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. CA-NFAT2

    Plasmid

    #11102

    Depositor

    Anjana Rao

    Article

    Monticelli et al Eur J Immunol. 2002 Oct . 32(10):2971-8.

    Insert

    NFAT2 (Nfatc1 Mouse)
    

    Use

    Retroviral

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Constitutively active: multiple serine to alanine…

    Promoter

    Available since

    April 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. SB100X in pCAG globin pA

    Plasmid

    #127909

    Purpose

    Sleeping Beauty Transposase expression vector driven by the CAG promoter

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    Sleeping Beauty Transposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-shRNA-ctrl

    Plasmid

    #85741

    Purpose

    shRNA ctrl

    Depositor

    Hongjun Song

    Article

    Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

    Insert

    shRNA ctrl
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmScarlet_C1

    Plasmid

    #85042

    Purpose

    Mammalian expression of mScarlet

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    mScarlet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL2-p21 promoter-Luc

    Plasmid

    #33021

    Depositor

    Martin Walsh

    Article

    Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

    Insert

    p21 promoter (CDKN1A Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    p21

    Available since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid

    #138147

    Purpose

    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLVX-UbC-rtTA-Ngn2:2A:EGFP

    Plasmid

    #127288

    Purpose

    all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

    Depositor

    Fred Gage

    Article

    Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

    Insert

    Ngn2:2A:Ascl1 (NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Ngn2 and EGFP linked via 2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. GFP-BRD4

    Plasmid

    #65378

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    R1232W

    Promoter

    CMV

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCasAb-apr

    Plasmid

    #121998

    Purpose

    Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSGAb-km

    Plasmid

    #121999

    Purpose

    A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Insert

    none
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pX333

    Plasmid

    #64073

    Purpose

    Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

    Depositor

    Andrea Ventura

    Article

    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    3XFLAG-Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CBh; U6

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. GSK3 beta pGEX

    Plasmid

    #15898

    Depositor

    Jim Woodgett

    Insert

    GSK3 beta (GSK3B Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. JD633

    Plasmid

    #160393

    Purpose

    CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Inserts

    ZmUbi::wheat GRF4-GIF1 chimera
    ZmUbi:TaCas9 + TaU6:gRNA
    

    Use

    Binary vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMVβ-p300-myc

    Plasmid

    #30489

    Purpose

    Mammalian expression of human p300 with myc tag

    Depositor

    Tso-Pang Yao

    Insert

    p300 (EP300 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFR_HCV_xb

    Plasmid

    #11510

    Depositor

    Phil Sharp

    Article

    Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

    Insert

    Firefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge