4,073 results

1. pBabe-Puro-IKBalpha-mut (super repressor)

   Plasmid

   #15291

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   inhibitor of Kappa B alpha (NFKBIA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   S32A/S36A: “super-repressor” allele (resistant to…

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pT7-EGFP-C1-HsNot1

   Plasmid

   #37370

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

   Insert

   HsNot1 (CNOT1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mPlum-N1

   Plasmid

   #54629

   Purpose

   Localization: N1 Cloning Vector, Excitation: 590, Emission: 649

   Depositor

   Michael Davidson

   Article

   Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

   Type

   Empty backbone

   Use

   Tags

   mPlum

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TRX1 flag

   Plasmid

   #21283

   Depositor

   Wang Min

   Article

   Liu et al Circ Res. 2002 Jun 28. 90(12):1259-66.

   Insert

   TRX1 (TXN Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 30, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 811 pSG5L HA PTEN C124S

   Plasmid

   #10744

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C124S

   Promoter

   Available since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-mDlx-GCaMP6f-Fishell-2

   Plasmid

   #83899

   Purpose

   GCaMP6f expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

   Depositor

   Gordon Fishell

   Article

   Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mDlx

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pWPT-PDX1

   Plasmid

   #12256

   Depositor

   Didier Trono

   Article

   Ritz-Laser et al Diabetologia. 2003 Jun . 46(6):810-21.

   Insert

   Pdx1 (PDX1 Human)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pWPXL-Sox9

   Plasmid

   #36979

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   Sox9 (Sox9 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-Tag2B EDD

   Plasmid

   #37188

   Depositor

   Charles Watts, Darren Saunders

   Article

   Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

   Insert

   EDD (UBR5 Human)
   

   Use

   Tags

   6x His Tag and FLAG

   Expression

   Mammalian

   Mutation

   Please see Depositor Comments

   Promoter

   CMV

   Available since

   Sept. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

    Plasmid

    #102245

    Purpose

    Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.

    Depositor

    Jacob Corn

    Insert

    dCas9-VPR-mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS-VPR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 12.4kbVillin-ΔATG

    Plasmid

    #19358

    Depositor

    Deborah Gumucio

    Article

    Madison et al J Biol Chem. 2002 Sep 6. 277(36):33275-8

    Insert

    12.4kb Villin promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    No ATG - transgenes cloned downstream of the vill…

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-E2F4

    Plasmid

    #10914

    Depositor

    Bob Weinberg

    Article

    Sardet et al Proc Natl Acad Sci U S A. 1995 Mar 14. 9

    Insert

    E2F4 (E2F4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

13. pDULE-ABK

    Plasmid

    #49086

    Purpose

    A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments

    Depositor

    Peter Schultz

    Article

    Ai et al Chembiochem. 2011 Aug 16;12(12):1854-7.

    Inserts

    tRNA synthetase
    tRNA for a photocrosslinking amino acid
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    L274M, C313A, Y349F

    Promoter

    GlnRS and lpp

    Available since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET_RTX

    Plasmid

    #102787

    Purpose

    Expresses the Reverse Transcriptase gene

    Depositor

    Andrew Ellington

    Article

    Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

    Insert

    reverse transcription xenopolymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter + lac operator

    Available since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CAV1-mEGFP

    Plasmid

    #27704

    Depositor

    Ari Helenius

    Article

    Hayer et al Traffic. 2010 Mar . 11(3):361-82.

    Insert

    caveolin-1 (CAV1 Canis lupus familiaris)
    

    Use

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMSCVgfp::AID

    Plasmid

    #15925

    Depositor

    Nina Papavasiliou

    Article

    Dickerson et al J Exp Med. 2003 May 19. 197(10):1291-6.

    Insert

    AID (AICDA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-DRD2

    Plasmid

    #24099

    Depositor

    Jean-Michel Arrang

    Article

    Jeanneteau et al Mol Biol Cell. 2004 Feb . 15(2):696-705.

    Insert

    DRD2 (DRD2 Human)
    

    Use

    Tags

    alpha7 nicotinic signal peptide and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FLAG-Foxo1ADA(pCMV5)

    Plasmid

    #12149

    Depositor

    Domenico Accili

    Article

    Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

    Insert

    Foxo1 ADA (Foxo1 Mouse)
    

    Use

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    Threonine 24 to Alanine (A) and Serine 253 to Asp…

    Promoter

    CMV

    Available since

    June 13, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pCFJ350 - MCS(ttTi5605, II)

    Plasmid

    #34866

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    ttTi5605 targeting
    

    Use

    Worm targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEM-HE-h_bPAC_cmyc

    Plasmid

    #28134

    Purpose

    optogenetic tool for light modulation of cellular cAMP

    Depositor

    Peter Hegemann

    Article

    Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

    Insert

    humanized photoactivated adenylyl cyclase
    

    Use

    In vitro transcription

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available since

    June 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSico PGK puro

    Plasmid

    #11586

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes the puromycin gene to be recombined out of the construct, activating shRNA expression.

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. ankG-mCherry

    Plasmid

    #42566

    Depositor

    Benedicte Dargent

    Article

    Leterrier et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    ankyrin G (Ank3 Rat)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-EGFP-Rac1-wt

    Plasmid

    #12980

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-hSyn-RFP

    Plasmid

    #22907

    Depositor

    Edward Callaway

    Article

    Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

    Insert

    human Synapsin I promoter driving DsRedExpress (SYN1 Human)
    

    Use

    AAV; Adeno-associated virus

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Ub-M-GFP

    Plasmid

    #11938

    Depositor

    Nico Dantuma

    Article

    Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

    Insert

    Ub-M-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ubiquitin fused to N-terminus of GFP. Methionine …

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AAV-FLEX-rev-ChR2(H134R)-mCherry

    Plasmid

    #18916

    Purpose

    Cre-dependent viral expression of ChR2(H134R)-mCherry for neuronal excitation

    Depositor

    Scott Sternson

    Article

    Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

    Insert

    Channelrhodopsin 2-mCherry
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    mCherry

    Expression

    Mutation

    H134R

    Promoter

    CAG

    Available since

    Jan. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pK-myc-Par3b

    Plasmid

    #19388

    Depositor

    Ian Macara

    Article

    Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

    Insert

    Partitioning-defective protein 3b (PARD3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Full length, isoform b.

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRK5-HA GST RagC wt

    Plasmid

    #19304

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Ras-related GTP binding C (RRAGC Human)
    

    Use

    Tags

    GST and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

    Plasmid

    #51085

    Purpose

    Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV Retrograde and AAV1

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin1

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MSCVhygro-F-G9a

    Plasmid

    #41721

    Purpose

    Retroviral expression of human G9a in mammalian cells

    Depositor

    Kai Ge

    Article

    Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

    Insert

    G9a (EHMT2 Human)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBabe-puro largeTcDNA

    Plasmid

    #14088

    Depositor

    William Hahn

    Article

    Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

    Insert

    Large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA-TRBP

    Plasmid

    #15666

    Depositor

    Dong-Yan Jin

    Article

    Kok et al J Biol Chem. 2007 Jun 15. 282(24):17649-

    Insert

    TRBP (TARBP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

33. pEP4 E02S EM2K

    Plasmid

    #20923

    Purpose

    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Myc and Klf4

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Oct4 and Sox2; Myc and Klf4 (SOX2, KLF4, MYC, POU5F1 Human)
    

    Use

    Episomal/ebna

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBABE hBRM

    Plasmid

    #1961

    Depositor

    Robert Kingston

    Article

    Sif et al Genes Dev 2001 Mar 1;15(5):603-18.

    Insert

    BRM (SMARCA2 Human)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3-DN-hCUL4B-FLAG

    Plasmid

    #15822

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin4B (CUL4B Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N594

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-HA-JMJD2A

    Plasmid

    #24180

    Purpose

    Mammalian expression of human JMJD2A with HA tag

    Depositor

    Kristian Helin

    Article

    Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

    Insert

    JMJD2A (KDM4A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. FU-tetO-Gateway

    Plasmid

    #43914

    Depositor

    John Gearhart

    Article

    Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHJ421

    Plasmid

    #20680

    Depositor

    Stephen Shaw

    Article

    Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

    Insert

    Ezrin (EZR Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCHERRY3

    Plasmid

    #24659

    Depositor

    Tanya Parish

    Article

    Carroll et al PLoS One. 2010 . 5(3):e9823.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLVX-EF1alpha-SARS-CoV-2-orf7a-2xStrep-IRES-Puro

    Plasmid

    #141388

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-orf7a (ORF7a SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge