175,573 results

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CD11b promoter in pGEM3zf(-)

Plasmid

#26168

Depositor

Daniel Tenen

Article

Dziennis et al Blood. 1995 Jan 15. 85(2):319-29.

Insert

CD11b promoter (ITGAM Human)

Expression

Bacterial

Available Since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-p53_R249S

Plasmid

#22935

Depositor

Bernard Futscher

Article

Junk et al Neoplasia. 2008 May . 10(5):450-61.

Insert

tumor protein 53 (TP53 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

R249S Mutant

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pNW55

Plasmid

#22988

Purpose

Expression of miRNA precursor for the osa-MIR528 locus in rice.

Depositor

Detlef Weigel

Article

Warthmann et al PLoS One. 2008 . 3(3):e1829.

Insert

osa-MIR528

Use

RNAi; Plant gene inactivation

Expression

Plant

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP39

Plasmid

#22581

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP39 (USP39 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only
Flag-HA-UCHL3

Plasmid

#22564

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

UCHL3 (UCHL3 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-HA-DUB3

Plasmid

#22593

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

DUB3 (USP17L9P Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK7B2Jnk2a2

Plasmid

#19727

Depositor

Roger Davis

Article

Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

Insert

pCDNA3 FlagMKK7B2Jnk2a2 (MAPK9, Map2k7 Human, Mouse)

Tags

Flag

Expression

Mammalian

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO HIS HSPA1A

Plasmid

#19537

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 1A (HSPA1A Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP C3-YAP2

Plasmid

#19055

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

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TRPML1-YFP

Plasmid

#18826

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML1 (MCOLN1 Human)

Tags

YFP

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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pLPC-NMYC TRF2

Plasmid

#16066

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Mutation

aa2-500, inserted alanin at aa434

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pBTG (pBigT-IRES-GFP; DM#268)

Plasmid

#15037

Depositor

Douglas Melton

Article

Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 10

Type

Empty backbone

Use

Cre/Lox; For making transgenic constructs

Tags

IRES-EGFP

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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IRES-GFP FoxG1

Plasmid

#14935

Depositor

Joan Massague

Article

Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

Insert

FoxG1 (FOXG1 Human)

Tags

IRES-GFP

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pSicoR human Drosha1

Plasmid

#14766

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Drosha shRNA (DROSHA Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA-DEST53 PINK1 N-GFP

Plasmid

#13315

Depositor

Mark Cookson

Article

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 (PINK1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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mTLR9

Plasmid

#13091

Depositor

Ruslan Medzhitov

Insert

TLR9 (Tlr9 Mouse)

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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SP (RSV) AP-2

Plasmid

#12100

Depositor

Robert Tjian

Article

Williams et al Genes Dev. 1991 Apr . 5(4):670-82.

Insert

AP-2 (TFAP2A Human)

Expression

Mammalian

Mutation

L2V

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-evoCAST-evoTnsAB

Plasmid

#234729

Purpose

Evolved human-codon optimized PseTnsAB in mammalian cells (for evoCAST)

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

evoPseTnsAB

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-GfaABC1D-MCS--4x6T-WPRE

Plasmid

#196417

Purpose

Empty vector for astrocyte-selective expression in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Type

Empty backbone

Use

AAV; Astrocyte-selective

Expression

Mammalian

Promoter

GfaABC1D

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDY0209 ACTB Nicking guide +48

Plasmid

#179109

Purpose

ACTB N-term Nicking guide 1 +48 guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

ACTB N-term Nicking guide 1 +48 guide

Use

CRISPR

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pZac2.1-GfaABC1D-TurboID(C )-HA-GPI

Plasmid

#163697

Purpose

Expresses c-terminal half of TurboID on surface of astrocytes

Depositor

Scott Soderling

Article

Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

Insert

GPI anchored TurboID

Use

AAV

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-mTagBFP2

Plasmid

#122373

Purpose

Expresses mTagBFP2 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

mTagBFP2

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro

Plasmid

#141387

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf6 (ORF6 SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf9c-IRES-Puro

Plasmid

#141393

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf9c

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCas9-sgAAVS1-2

Plasmid

#129727

Purpose

Expressing SpCas9 and sgAAVS1-2

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

SpCas9 and sgAAVS1-2

Use

CRISPR

Tags

T2A-mCherry-P2A-Puro

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSRE SSG

Plasmid

#127692

Purpose

Plasmid expresses the Sindbis Structural Genome (SSG) via the serum response element (SRE) promoter. See Resource Information section for annotated plasmid sequence.

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

Sindbis Structural Genome

Expression

Mammalian

Available Since

July 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SpyDock

Plasmid

#124618

Purpose

Expresses SpyDock to bind SpyTag non-covalently for affinity purification

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyDock

Tags

His6

Expression

Bacterial

Mutation

E77A mutation prevents isopeptide bond formation,…

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pZac2.1-GfaABC1D-Rpl22-HA

Plasmid

#111811

Purpose

Express Rpl22HA in astrocytes

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Ribosomal protein L22 (Rpl22 Mouse)

Tags

HA

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Voltron

Plasmid

#119033

Purpose

Express Voltron in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
HIVGKO

Plasmid

#112234

Purpose

HIVGKO contains a codon-switched eGFP under the control of the HIV-1 specific promoter, and mKO2 under the control of the constitutive promoter EF1a

Depositor

Eric Verdin

Article

Battivelli et al Elife. 2018 May 1;7. pii: 34655. doi: 10

Insert

csGFP-EF1a-mKO2

Use

Lentiviral

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSmartBAC-S

Plasmid

#107268

Purpose

Broad host range bacterial artificial chromosome vector

Depositor

Ron Godiska, Mark Liles

Article

Nasrin et al J Nat Prod. 2018 Jun 22;81(6):1321-1332.

Type

Empty backbone

Expression

Bacterial

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG cyto-RCaMP1h

Plasmid

#105014

Purpose

cytosolic calcium sensor

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

RCaMP1h

Expression

Mammalian

Promoter

pCAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW62.YY1.FKBP.knock-in.mCherry

Plasmid

#104370

Purpose

For knocking in FKBP F36V P2A mCherry onto YY1

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

FKBP12(F36V) -P2A-mCherry

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pC0052-REPAIR non-targeting guide clone into pC0043

Plasmid

#103868

Purpose

Non-targeting guide RNA for use with PspCas13b. Useful as a control for RNA editing and RNA knockdown experiments.

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

non-targeting guide RNA

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEFIRES-P-ACSL3-mCherry

Plasmid

#87158

Purpose

Fluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

ACSL3 (ACSL3 Human)

Tags

mCherry

Expression

Mammalian

Promoter

EF-1-alpha

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A

Plasmid

#86674

Purpose

Expresses catalytically-inactive GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

GFP-FLAG-cGAS E225A/D227A

Use

Lentiviral

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLS-mP

Plasmid

#81225

Purpose

Lentivirus enhancer assay plasmid that contains a minimal promoter (mP) and a EGFP reporter gene.

Depositor

Nadav Ahituv

Article

Inoue et al Genome Res. 2016 Nov 9. pii: gr.212092.1

Type

Empty backbone

Use

Lentiviral

Promoter

Derived from pGL4.23

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
SQT1659

Plasmid

#78743

Purpose

Human expression plasmid for AsCpf1-NLS-3xHA

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

mammalian codon-optimized Cpf1-NLS-3xHA

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CAG

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMJ922

Plasmid

#78312

Purpose

Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli

Depositor

Martin Jinek

Article

Burger et al Development. 2016 Apr 29. pii: dev.13480

Insert

SpCas9

Tags

HA-2xNLS-EGFP-NLS and His6-MBP-TEV

Expression

Bacterial

Mutation

human codon-optimized gene sequence

Promoter

T7

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pNCS-Antares

Plasmid

#74279

Purpose

Constitutive expression of Antares in bacteria

Depositor

Michael Lin

Article

Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

Antares

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-Sac-Abeta(M1-40)

Plasmid

#71876

Purpose

Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

Depositor

Dominic Walsh

Article

Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

Insert

Abeta(M1-40) (APP Human)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-RhoA Biosensor

Plasmid

#68026

Purpose

C-terminus of Anillin in mammalian expression vector pEGFP-C1

Depositor

Michael Glotzer

Article

Piekny et al Curr Biol. 2008 Jan 8;18(1):30-6. Epub 2

Insert

Anillin (AHD+PH) (ANLN Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTQL1211YN

Plasmid

#61704

Purpose

Gateway compatible BiFC vector (pEarleyGate201-YN)

Depositor

Yuhai Cui

Article

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Use

Bifc assay

Tags

YFP-N and HA

Expression

Plant

Promoter

35S

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4TO-H2B-SunTag24x_v1

Plasmid

#60911

Purpose

Encodes a histone H2B fused to the SunTag for single molecule imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

H2B

Expression

Mammalian

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330S-3

Plasmid

#58779

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMZ374

Plasmid

#59896

Purpose

Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).

Depositor

Mikel Zaratiegui

Article

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Expression

Yeast

Mutation

Silent muation of the CspCI site

Promoter

adh1 and rrk1

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX304-FOS-V5

Plasmid

#59140

Purpose

wild-type FOS with C-terminal V5 tag expressed from pLX vector

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

FOS (FOS Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Centrin2-N-10

Plasmid

#55018

Purpose

Localization: Centrosomes, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Centrin2 (CETN2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
DsRed2-pBAD

Plasmid

#54608

Purpose

Localization: Protein Expression Vector , Excitation: 558, Emission: 583

Depositor

Michael Davidson

Type

Empty backbone

Tags

6xHis and DsRed2

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
ZsGreen1-N1

Plasmid

#54702

Purpose

Localization: N1 Cloning Vector, Excitation: 493, Emission: 505

Depositor

Michael Davidson

Type

Empty backbone

Tags

ZsGreen1

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge

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