We narrowed to 6,909 results for: CAD
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Plasmid#164971PurposeExpresses POLArISact, an orientation probe that specifically binds to F-actin, in mammalian cellsDepositorInsertPOLArISact
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBC2-null
Plasmid#162749PurposeMSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genesDepositorInsertmurine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBC1-null
Plasmid#162748PurposeMSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genesDepositorInsertmurine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJR021
Plasmid#198814PurposeVariant of yellow fluorescent protein for transient gene expressionDepositorInsert15xUAS::destabilizedYFP::let-838 3'UTR
UseTagsExpressionWormMutationYFP with destabilized signal from mouse ornithine…PromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Beclin1 243-450
Plasmid#24392DepositorInsertBeclin1 (BECN1 Human)
UseTagsFLAGExpressionMammalianMutationContains amino acids 243-450.PromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
BtuF
Plasmid#92209PurposeExpression of BtuF which is the vitamin B12 binding protein for BtuCD ABC TransporterDepositorInsertBtuF (btuF )
UseTags6x His Tag and PelB Signal SequenceExpressionBacterialMutationBtuF signal sequence removed and uses the PelB si…PromoterT7 PromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Beclin1 151-241
Plasmid#24390DepositorInsertBeclin1 (BECN1 Human)
UseTagsFLAGExpressionMammalianMutationContains amino acids 151-241.PromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
896 pcDNA3 T7 Akt1
Plasmid#9003DepositorInsertAkt1 (AKT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
Per2:iLuc
Plasmid#160293Purposetarget Per2 gene with a color-switching luciferase cassette in mouse genome and the transfection of the recombinant DNA will lead genomic recombination and make a knock-in allele in mouse ES cells.DepositorInsertPer2LUC
UseTagsExpressionMammalianMutationPromoterno promotorAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-tdTomato-cNLS
Plasmid#138532PurposeLentiviral inducible expression of tdTomato with nuclear localization signalDepositorInserttdTomato
UseLentiviralTags3x SV40 cNLSExpressionMammalianMutationPromoterTRE-CMVAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A1
Plasmid#135503PurposeMammalian expression of myc-tagged HLA-A*01:01DepositorInsertHLA-A*01:01 (HLA-A Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV10-Flag-Fbxl21
Plasmid#48752PurposeExpression of FLAG-tagged Fbxl21DepositorInsertFbxl21 (Fbxl21 Mouse)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag mHMGB2
Plasmid#31610DepositorInsertHMGB2 (Hmgb2 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLux01
Plasmid#78281PurposemRFP1 expressed behind an AHL-inducible constructDepositorInsertAHL inducible mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1
Plasmid#107407PurposeLentiviral expression of gRNA resistant PBRM1DepositorInsertsg resistant PBRM1 (PBRM1 Human)
UseLentiviralTagsExpressionMutationsgRNA resistantPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2-5ptaseOCRL(D523G)
Plasmid#66837PurposeExpression of inositol 5-phosphatase domain of OCRL(D523G - catalytically inactive mutant). Binds to CIBN upon blue light illumination.DepositorInsert5ptaseOCRL (OCRL Human)
UseTagsmCherry-CRY2ExpressionMammalianMutationD523GPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV.PGK.mLmx1a
Plasmid#33013DepositorInsertLIM homeobox transcription factor 1 alpha (Lmx1a Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pWPI-IRES-eGFP-MEFV
Plasmid#92103PurposeExpression in eukaryotic cells and generating stable cell linesDepositorInsertMEFV (MEFV Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18
Plasmid#172316PurposeCRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Rab10wt
Plasmid#135557PurposeExpress mouse Rab10wt in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Rab10wt.DepositorInsertRab10 (Rab10 Mouse)
UseTags6xHis tag, TEV siteExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
E42_gRunx1_gRNA2_dTet_NGFR
Plasmid#189801PurposeRetroviral delivery of guide RNA against mouse Runx1DepositorInsertgRunx1_gRNA2 (Runx1 Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
E42_gRunx1_gRNA1_dTet_NGFR
Plasmid#189802PurposeRetroviral delivery of guide RNA against mouse Runx1DepositorInsertgRunx1_gRNA1 (Runx1 Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMVhPOP1_3Flag
Plasmid#53968PurposeMammalian expression vector for human POP1 with c-terminal 3xFLAG epitopes.DepositorInsertPOP1 (POP1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Beclin1-(151-241)
Plasmid#24393DepositorInsertBeclin1 (BECN1 Human)
UseTagsFLAGExpressionMammalianMutationAmino acids 151-241 are deleted. Delta CC mutant.PromoterAvailable sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJS145
Plasmid#179159PurposeEncodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.DepositorInsertrps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
UseTagsExpressionWormMutationFlexon replacing 2nd intron of 2xNLS::GFPPromoterrps-27pAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-N90-beta-catenin
Plasmid#31787DepositorInsertbeta-catenin (CTNNB1 Human)
UseEntry vectorTagsMycExpressionMutationDeletion of aa 1-90- Constitutively ActivePromoterAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
sgPBRM1-1- LentiCRISPRv2
Plasmid#107403PurposeLentiviral expression of Cas9 and gRNA targeting PBRM1DepositorInsertgRNA targeting PBRM1 (PBRM1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-CNL
Plasmid#65712PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-CNL)DepositorInsert7xTcf, minCMV, 3xNLS, CNL
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
sgPBRM1-2- LentiCRISPRv2
Plasmid#107404PurposeLentiviral expression of Cas9 and gRNA targeting PBRM1DepositorInsertgRNA targeting PBRM1 (PBRM1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-WT (AF41)
Plasmid#21367DepositorInsertautosomal dominant polycystic kidney disease type I (PKD1 Human)
UseTagsEGFP and FLAGExpressionMammalianMutationwild-type sequence*PromoterAvailable sinceDec. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCI syn iGluu
Plasmid#106122PurposeFast indicator for synaptic glutamate imaging (synapsin promoter)DepositorInsertiGluSnFR S72T variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Ser 72 to ThrPromotersynapsinAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe human FOXC2
Plasmid#15535DepositorInsertFOXC2 (FOXC2 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delCAT
Plasmid#173942PurposeBacterial expression of PARP1 lacking CAT domainDepositorInsertPARP1delCAT (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 661-1014PromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9
Plasmid#186699PurposePlasmid encoding PRDM9dC-Cas9 fusion under CMV promoterDepositorInsertPRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9 (PRDM9 Human)
UseCRISPRTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV3Tag8li-hDDX50-HA
Plasmid#165465PurposeHuman DDX50 mammalian expression vector with C-terminal HA tag.DepositorInsertDExD-box helicase 50 (DDX50 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ad-Flag-XBP1u
Plasmid#63677PurposeAdenoviral vector for XBP1u expressionDepositorInsertXBP1 (XBP1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutationunsplicedPromoterAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-rat ERK2-WT
Plasmid#40812DepositorInsertMAPK1 (Mapk1 Rat)
UseTagsHis6ExpressionMammalianMutationwild typePromoterCMVAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
aav-tnt-dnmrtfa-gfp
Plasmid#165035PurposeAAV-based delivery of dnMRTFA-GFP into cardiomyocytes in vivoDepositorInsertdnMRTFA
UseAAVTagsGFPExpressionMutationdeletion of 100 N-terminal amino acidsPromotercTNTAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHeEf1a-mEGFP-NLS
Plasmid#205012PurposeThe vector contains 1kbp of the upstream and downstream regions of the ef1a gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the ef1a gene from Hypsibius exemplaris
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvACT-mEGFP-NLS
Plasmid#205001PurposeThe vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe HA-Runx1-neo
Plasmid#45815DepositorInsertRunx1 (Runx1 Mouse)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delWGR
Plasmid#173940PurposeBacterial expression of PARP1 lacking WGR domainDepositorInsertPARP1delWGR (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 521-660; V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Myc-CYLD-C601A
Plasmid#60027PurposeMyc-tagged CYLD catalytic inactive (C601A) expressionDepositorInsertCylindromatosis (CYLD Human)
UseTagsMycExpressionMammalianMutationCysteine 601 changed to AlaninePromoterCMVAvailable sinceNov. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pLEX_305-N-dTAG_OGT
Plasmid#155196PurposeN-terminal FKBP12(F36V)-2xHA tag on lentiviral OGT constructDepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x HA and FKBP12(F36V)ExpressionMammalianMutationlacking N-terminal MetPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pTetOne-Six1-myc
Plasmid#138530PurposeInducible expression of Six1 with a myc tagDepositorInsertSix1 (Six1 Mouse)
UseTags3x myc tagExpressionMammalianMutationPromoterTRE3GsAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(800-868)
Plasmid#141345PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868).DepositorInsertGB1His6-TEV-ALIX(800-868)
UseTagsGB1-His6-TEVExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-split-intein-Gal4[1-20]-N-int
Plasmid#199195PurposeInsert to create split-intein Gal4[N-int] knock-in vectors via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-split-intein-Gal4[1-20]-N-int, 3xP3-dsRed
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only